Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China

Yong Teng, H. Liu, J. Q. Lv, W. H. Fan, Q. Y. Zhang, Q. W. Qin

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72 Citations (Scopus)

Abstract

The complete genome of spring viraemia of carp virus (SVCV) strain A-1 isolated from cultured common carp (Cyprinus carpio) in China was sequenced and characterized. Reverse transcription-polymerase chain reaction (RT-PCR) derived clones were constructed and the DNA was sequenced. It showed that the entire genome of SVCV A-1 consists of 11,100 nucleotide base pairs, the predicted size of the viral RNA of rhabdoviruses. However, the additional insertions in bp 4633-4676 and bp 4684-4724 of SVCV A-1 were different from the other two published SVCV complete genomes. Five open reading frames (ORFs) of SVCV A-1 were identified and further confirmed by RT-PCR and DNA sequencing of their respective RT-PCR products. The 5 structural proteins encoded by the viral RNA were ordered 3′-N-P-M-G-L-5′. This is the first report of a complete genome sequence of SVCV isolated from cultured carp in China. Phylogenetic analysis indicates that SVCV A-1 is closely related to the members of the genus Vesiculovirus, family Rhabdoviridae.

Original languageEnglish (US)
Pages (from-to)1457-1465
Number of pages9
JournalArchives of Virology
Volume152
Issue number8
DOIs
StatePublished - Aug 1 2007

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Carps
Viremia
China
Genome
Viruses
Rhabdoviridae
Reverse Transcription
Viral RNA
Polymerase Chain Reaction
Vesiculovirus
DNA Sequence Analysis
Base Pairing
Open Reading Frames
Nucleotides
Clone Cells

ASJC Scopus subject areas

  • Virology

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Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China. / Teng, Yong; Liu, H.; Lv, J. Q.; Fan, W. H.; Zhang, Q. Y.; Qin, Q. W.

In: Archives of Virology, Vol. 152, No. 8, 01.08.2007, p. 1457-1465.

Research output: Contribution to journalArticle

Teng, Yong ; Liu, H. ; Lv, J. Q. ; Fan, W. H. ; Zhang, Q. Y. ; Qin, Q. W. / Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China. In: Archives of Virology. 2007 ; Vol. 152, No. 8. pp. 1457-1465.
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