Characterization of GM 1 ganglioside by direct inlet chemical ionization mass spectrometry

T. Ariga, Robert K Yu, M. Suzuki

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Intact permethylated and permethylated-reduced (LiAlH 4 ) derivatives of GM 1 ganglioside were analyzed by direct inlet ammonia chemical ionization (CI) mass spectrometry. In addition, the trimethylsilylated derivative of the permethylated-reduced sample of this ganglioside was similarly analyzed. CI mass spectrometry proves to be a highly satisfactory method for structural studies of GM 1 ganglioside because of the simple fragmentation pattern and the presence of prominent molecular ions and fragment ions in the high mass region. Complete information on the carbohydrate sequence and lipophilic composition can be easily obtained.

Original languageEnglish (US)
Pages (from-to)437-442
Number of pages6
JournalJournal of Lipid Research
Volume23
Issue number3
StatePublished - Jan 1 1982
Externally publishedYes

Fingerprint

Gangliosides
Ionization
Mass spectrometry
Mass Spectrometry
Carbohydrate Sequence
Ions
Derivatives
Ammonia
Carbohydrates
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Characterization of GM 1 ganglioside by direct inlet chemical ionization mass spectrometry . / Ariga, T.; Yu, Robert K; Suzuki, M.

In: Journal of Lipid Research, Vol. 23, No. 3, 01.01.1982, p. 437-442.

Research output: Contribution to journalArticle

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