Characterization of human FHL2 transcript variants and gene expression regulation in hepatocellular carcinoma

Chor Fung Ng, Wayne Jun Wei Zhou, Patrick Kwok Shing Ng, Man Shan Li, Yuen Keng Ng, Paul Bo San Lai, Stephen Kwok Wing Tsui

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The four-and-a-half LIM protein 2 (FHL2) was originally identified to be expressed abundantly in the heart, as well as in a wide range of tissues demonstrated in various studies. The human FHL2 gene expresses different transcripts which are known to differ only in the 5'UTR region. However, little is known about the functional role of the different variants and the mechanism of gene regulation. In the present study, we characterized the different alternative spliced transcripts of FHL2 by in silico analysis and RT-PCR analysis. A novel transcript variant was identified. The FHL2 gene produces transcripts by different 5' exons, which may be responsible for tissue-specific regulation. To study the mechanism of FHL2 gene regulation, the potential promoter region was investigated. We have identified a functional promoter region upstream of the transcription start site. Deletion mutation analysis of 5' flanking region showed that the fragment from '. 138 to +. 292. bp have positive regulatory effect. We identified the binding sites of Pax-5/ZF5 in this region and found that Pax-5 and ZF5 expression in HCC samples had a significant positive correlation with FHL2 expression, suggesting a possible role for these transcription factors in the regulation of FHL2 expression.

Original languageEnglish (US)
Pages (from-to)41-47
Number of pages7
JournalGene
Volume481
Issue number1
DOIs
StatePublished - Jul 15 2011
Externally publishedYes

Keywords

  • FHL2
  • Liver cancer
  • Promoter
  • Splice variants
  • Transcription factor binding sites

ASJC Scopus subject areas

  • Genetics

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