Abstract
Human protein 1/ Clara cell Mr 10,000 protein consists of two identical subunits of seventy amino acid residues each. In the present study, eight clones of monoclonal antibodies against native protein 1 were prepared and their respective epitopes were immunochemically and immunohistochemically characterized using native protein I, truncated recombinant protein 1 and synthesized peptides. Among the clones, three designated as TY-5, TY-7 and TY-8 recognized amino acid residues 7-16, residues 19-28, and residues 39-46, respectively, all of which comprise the hydrophobic cavity of protein 1, possibly associated with chemical binding function. With the exception of TY-4, the remaining clones recognized residues 61-68 which are exposed to solvent. The epitope of TY-4 remains undetermined. Proper selection and combination of clones and recombinant protein 1 may be useful for fundamental and clinical studies of protein 1.
Original language | English (US) |
---|---|
Pages (from-to) | 631-637 |
Number of pages | 7 |
Journal | Clinical Chemistry and Laboratory Medicine |
Volume | 37 |
Issue number | 6 |
DOIs | |
State | Published - Sep 1 1999 |
Externally published | Yes |
Keywords
- Clara cell 10 kilodalton protein
- Epitope
- Human protein 1
- Monoclonal antibody
- Truncated recombinant protein
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical