Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein

Tetsuji Yamaguchi, Toshiyuki Yamada, Ryuta Okutani, Noriharu Shijubo, Gurmukh Singh, Yoshihisa Itoh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human protein 1/ Clara cell Mr 10,000 protein consists of two identical subunits of seventy amino acid residues each. In the present study, eight clones of monoclonal antibodies against native protein 1 were prepared and their respective epitopes were immunochemically and immunohistochemically characterized using native protein I, truncated recombinant protein 1 and synthesized peptides. Among the clones, three designated as TY-5, TY-7 and TY-8 recognized amino acid residues 7-16, residues 19-28, and residues 39-46, respectively, all of which comprise the hydrophobic cavity of protein 1, possibly associated with chemical binding function. With the exception of TY-4, the remaining clones recognized residues 61-68 which are exposed to solvent. The epitope of TY-4 remains undetermined. Proper selection and combination of clones and recombinant protein 1 may be useful for fundamental and clinical studies of protein 1.

Original languageEnglish (US)
Pages (from-to)631-637
Number of pages7
JournalClinical Chemistry and Laboratory Medicine
Volume37
Issue number6
DOIs
StatePublished - Sep 1 1999

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Monoclonal Antibodies
Clone Cells
Proteins
Recombinant Proteins
Epitopes
Amino Acids
Peptides

Keywords

  • Clara cell 10 kilodalton protein
  • Epitope
  • Human protein 1
  • Monoclonal antibody
  • Truncated recombinant protein

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein. / Yamaguchi, Tetsuji; Yamada, Toshiyuki; Okutani, Ryuta; Shijubo, Noriharu; Singh, Gurmukh; Itoh, Yoshihisa.

In: Clinical Chemistry and Laboratory Medicine, Vol. 37, No. 6, 01.09.1999, p. 631-637.

Research output: Contribution to journalArticle

Yamaguchi, Tetsuji ; Yamada, Toshiyuki ; Okutani, Ryuta ; Shijubo, Noriharu ; Singh, Gurmukh ; Itoh, Yoshihisa. / Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein. In: Clinical Chemistry and Laboratory Medicine. 1999 ; Vol. 37, No. 6. pp. 631-637.
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