Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein

Tetsuji Yamaguchi, Toshiyuki Yamada, Ryuta Okutani, Noriharu Shijubo, Gurmukh Singh, Yoshihisa Itoh

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Human protein 1/ Clara cell Mr 10,000 protein consists of two identical subunits of seventy amino acid residues each. In the present study, eight clones of monoclonal antibodies against native protein 1 were prepared and their respective epitopes were immunochemically and immunohistochemically characterized using native protein I, truncated recombinant protein 1 and synthesized peptides. Among the clones, three designated as TY-5, TY-7 and TY-8 recognized amino acid residues 7-16, residues 19-28, and residues 39-46, respectively, all of which comprise the hydrophobic cavity of protein 1, possibly associated with chemical binding function. With the exception of TY-4, the remaining clones recognized residues 61-68 which are exposed to solvent. The epitope of TY-4 remains undetermined. Proper selection and combination of clones and recombinant protein 1 may be useful for fundamental and clinical studies of protein 1.

Original languageEnglish (US)
Pages (from-to)631-637
Number of pages7
JournalClinical Chemistry and Laboratory Medicine
Volume37
Issue number6
DOIs
StatePublished - Sep 1 1999
Externally publishedYes

Keywords

  • Clara cell 10 kilodalton protein
  • Epitope
  • Human protein 1
  • Monoclonal antibody
  • Truncated recombinant protein

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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