Abstract
Human protein 1/ Clara cell Mr 10,000 protein consists of two identical subunits of seventy amino acid residues each. In the present study, eight clones of monoclonal antibodies against native protein 1 were prepared and their respective epitopes were immunochemically and immunohistochemically characterized using native protein I, truncated recombinant protein 1 and synthesized peptides. Among the clones, three designated as TY-5, TY-7 and TY-8 recognized amino acid residues 7-16, residues 19-28, and residues 39-46, respectively, all of which comprise the hydrophobic cavity of protein 1, possibly associated with chemical binding function. With the exception of TY-4, the remaining clones recognized residues 61-68 which are exposed to solvent. The epitope of TY-4 remains undetermined. Proper selection and combination of clones and recombinant protein 1 may be useful for fundamental and clinical studies of protein 1.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 631-637 |
| Number of pages | 7 |
| Journal | Clinical Chemistry and Laboratory Medicine |
| Volume | 37 |
| Issue number | 6 |
| DOIs | |
| State | Published - Sep 1 1999 |
Fingerprint
Keywords
- Clara cell 10 kilodalton protein
- Epitope
- Human protein 1
- Monoclonal antibody
- Truncated recombinant protein
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical
Cite this
Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein. / Yamaguchi, Tetsuji; Yamada, Toshiyuki; Okutani, Ryuta; Shijubo, Noriharu; Singh, Gurmukh; Itoh, Yoshihisa.
In: Clinical Chemistry and Laboratory Medicine, Vol. 37, No. 6, 01.09.1999, p. 631-637.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein
AU - Yamaguchi, Tetsuji
AU - Yamada, Toshiyuki
AU - Okutani, Ryuta
AU - Shijubo, Noriharu
AU - Singh, Gurmukh
AU - Itoh, Yoshihisa
PY - 1999/9/1
Y1 - 1999/9/1
N2 - Human protein 1/ Clara cell Mr 10,000 protein consists of two identical subunits of seventy amino acid residues each. In the present study, eight clones of monoclonal antibodies against native protein 1 were prepared and their respective epitopes were immunochemically and immunohistochemically characterized using native protein I, truncated recombinant protein 1 and synthesized peptides. Among the clones, three designated as TY-5, TY-7 and TY-8 recognized amino acid residues 7-16, residues 19-28, and residues 39-46, respectively, all of which comprise the hydrophobic cavity of protein 1, possibly associated with chemical binding function. With the exception of TY-4, the remaining clones recognized residues 61-68 which are exposed to solvent. The epitope of TY-4 remains undetermined. Proper selection and combination of clones and recombinant protein 1 may be useful for fundamental and clinical studies of protein 1.
AB - Human protein 1/ Clara cell Mr 10,000 protein consists of two identical subunits of seventy amino acid residues each. In the present study, eight clones of monoclonal antibodies against native protein 1 were prepared and their respective epitopes were immunochemically and immunohistochemically characterized using native protein I, truncated recombinant protein 1 and synthesized peptides. Among the clones, three designated as TY-5, TY-7 and TY-8 recognized amino acid residues 7-16, residues 19-28, and residues 39-46, respectively, all of which comprise the hydrophobic cavity of protein 1, possibly associated with chemical binding function. With the exception of TY-4, the remaining clones recognized residues 61-68 which are exposed to solvent. The epitope of TY-4 remains undetermined. Proper selection and combination of clones and recombinant protein 1 may be useful for fundamental and clinical studies of protein 1.
KW - Clara cell 10 kilodalton protein
KW - Epitope
KW - Human protein 1
KW - Monoclonal antibody
KW - Truncated recombinant protein
UR - http://www.scopus.com/inward/record.url?scp=0032858207&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032858207&partnerID=8YFLogxK
U2 - 10.1515/CCLM.1999.098
DO - 10.1515/CCLM.1999.098
M3 - Article
C2 - 10475070
AN - SCOPUS:0032858207
VL - 37
SP - 631
EP - 637
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
SN - 1434-6621
IS - 6
ER -