Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus

Qing Sheng Mi, Li Zhou, Marsha Grattan, Zai Zhao Wang, M. Sivilotti, Jin-Xiong She, Terry L. Delovitch

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We recently mapped Idd4 to a 5.2 cM interval on chromosome 11 with two subloci, Idd4.1 and Idd4.2, in nonobese diabetic (NOD) mice. Based on the localization of platelet-activating factor acetylhydrolase Ib1 (PAF-AHIb1) and the decreased activity of PAF-AH in type 1 diabetes (T1D) patients, we hypothesized that PAF-AHIb1 in Idd4.1 is a candidate gene. The PAF-AHIb1 gene in NOD mice was cloned and sequenced, and its expression and function were studied. No polymorphisms were detected in PAF-AHIb1 cDNA between NOD and B6 mice. The expression of PAF-AH Ib1 at the mRNA and protein levels was found to be similar in different tissues between NOD and B6 mice. PAF-AH activity does not differ in the pancreatic islets or spleen between NOD and B6 mice. Our findings suggest that PAF-AH Ib1 may not be a diabetes-susceptibility gene in the Idd4.1 sublocus.

Original languageEnglish (US)
Title of host publicationImmunology of Diabetes IV
Subtitle of host publicationProgress in Our Understanding
PublisherBlackwell Publishing Inc.
Pages147-152
Number of pages6
ISBN (Print)1573316415, 9781573316415
DOIs
StatePublished - Oct 2006

Publication series

NameAnnals of the New York Academy of Sciences
Volume1079
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Diabetes
  • Genetic susceptibility
  • Idd4
  • Nonobese diabetic mice
  • Platelet-activating factor acetylhydrolase Ib1 gene

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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