Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus

Qing Sheng Mi, Li Zhou, Marsha Grattan, Zai Zhao Wang, M. Sivilotti, Jin-Xiong She, Terry L. Delovitch

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We recently mapped Idd4 to a 5.2 cM interval on chromosome 11 with two subloci, Idd4.1 and Idd4.2, in nonobese diabetic (NOD) mice. Based on the localization of platelet-activating factor acetylhydrolase Ib1 (PAF-AHIb1) and the decreased activity of PAF-AH in type 1 diabetes (T1D) patients, we hypothesized that PAF-AHIb1 in Idd4.1 is a candidate gene. The PAF-AHIb1 gene in NOD mice was cloned and sequenced, and its expression and function were studied. No polymorphisms were detected in PAF-AHIb1 cDNA between NOD and B6 mice. The expression of PAF-AH Ib1 at the mRNA and protein levels was found to be similar in different tissues between NOD and B6 mice. PAF-AH activity does not differ in the pancreatic islets or spleen between NOD and B6 mice. Our findings suggest that PAF-AH Ib1 may not be a diabetes-susceptibility gene in the Idd4.1 sublocus.

Original languageEnglish (US)
Title of host publicationImmunology of Diabetes IV
Subtitle of host publicationProgress in Our Understanding
PublisherBlackwell Publishing Inc.
Pages147-152
Number of pages6
ISBN (Print)1573316415, 9781573316415
DOIs
StatePublished - Jan 1 2006

Publication series

NameAnnals of the New York Academy of Sciences
Volume1079
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Fingerprint

Inbred NOD Mouse
Platelet Activating Factor
Medical problems
Genes
Chromosomes
Polymorphism
Chromosomes, Human, Pair 11
Type 1 Diabetes Mellitus
Islets of Langerhans
Complementary DNA
Tissue
Spleen
Messenger RNA
Susceptibility
Mouse
Diabetes
Locus
Gene
Proteins

Keywords

  • Diabetes
  • Genetic susceptibility
  • Idd4
  • Nonobese diabetic mice
  • Platelet-activating factor acetylhydrolase Ib1 gene

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Mi, Q. S., Zhou, L., Grattan, M., Wang, Z. Z., Sivilotti, M., She, J-X., & Delovitch, T. L. (2006). Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus. In Immunology of Diabetes IV: Progress in Our Understanding (pp. 147-152). (Annals of the New York Academy of Sciences; Vol. 1079). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1375.022

Characterization of PAF-AH Ib1 in NOD mice : PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus. / Mi, Qing Sheng; Zhou, Li; Grattan, Marsha; Wang, Zai Zhao; Sivilotti, M.; She, Jin-Xiong; Delovitch, Terry L.

Immunology of Diabetes IV: Progress in Our Understanding. Blackwell Publishing Inc., 2006. p. 147-152 (Annals of the New York Academy of Sciences; Vol. 1079).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mi, QS, Zhou, L, Grattan, M, Wang, ZZ, Sivilotti, M, She, J-X & Delovitch, TL 2006, Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus. in Immunology of Diabetes IV: Progress in Our Understanding. Annals of the New York Academy of Sciences, vol. 1079, Blackwell Publishing Inc., pp. 147-152. https://doi.org/10.1196/annals.1375.022
Mi QS, Zhou L, Grattan M, Wang ZZ, Sivilotti M, She J-X et al. Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus. In Immunology of Diabetes IV: Progress in Our Understanding. Blackwell Publishing Inc. 2006. p. 147-152. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1375.022
Mi, Qing Sheng ; Zhou, Li ; Grattan, Marsha ; Wang, Zai Zhao ; Sivilotti, M. ; She, Jin-Xiong ; Delovitch, Terry L. / Characterization of PAF-AH Ib1 in NOD mice : PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus. Immunology of Diabetes IV: Progress in Our Understanding. Blackwell Publishing Inc., 2006. pp. 147-152 (Annals of the New York Academy of Sciences).
@inproceedings{34e17cf75c994ad7935c4716a7ea4cde,
title = "Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus",
abstract = "We recently mapped Idd4 to a 5.2 cM interval on chromosome 11 with two subloci, Idd4.1 and Idd4.2, in nonobese diabetic (NOD) mice. Based on the localization of platelet-activating factor acetylhydrolase Ib1 (PAF-AHIb1) and the decreased activity of PAF-AH in type 1 diabetes (T1D) patients, we hypothesized that PAF-AHIb1 in Idd4.1 is a candidate gene. The PAF-AHIb1 gene in NOD mice was cloned and sequenced, and its expression and function were studied. No polymorphisms were detected in PAF-AHIb1 cDNA between NOD and B6 mice. The expression of PAF-AH Ib1 at the mRNA and protein levels was found to be similar in different tissues between NOD and B6 mice. PAF-AH activity does not differ in the pancreatic islets or spleen between NOD and B6 mice. Our findings suggest that PAF-AH Ib1 may not be a diabetes-susceptibility gene in the Idd4.1 sublocus.",
keywords = "Diabetes, Genetic susceptibility, Idd4, Nonobese diabetic mice, Platelet-activating factor acetylhydrolase Ib1 gene",
author = "Mi, {Qing Sheng} and Li Zhou and Marsha Grattan and Wang, {Zai Zhao} and M. Sivilotti and Jin-Xiong She and Delovitch, {Terry L.}",
year = "2006",
month = "1",
day = "1",
doi = "10.1196/annals.1375.022",
language = "English (US)",
isbn = "1573316415",
series = "Annals of the New York Academy of Sciences",
publisher = "Blackwell Publishing Inc.",
pages = "147--152",
booktitle = "Immunology of Diabetes IV",

}

TY - GEN

T1 - Characterization of PAF-AH Ib1 in NOD mice

T2 - PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus

AU - Mi, Qing Sheng

AU - Zhou, Li

AU - Grattan, Marsha

AU - Wang, Zai Zhao

AU - Sivilotti, M.

AU - She, Jin-Xiong

AU - Delovitch, Terry L.

PY - 2006/1/1

Y1 - 2006/1/1

N2 - We recently mapped Idd4 to a 5.2 cM interval on chromosome 11 with two subloci, Idd4.1 and Idd4.2, in nonobese diabetic (NOD) mice. Based on the localization of platelet-activating factor acetylhydrolase Ib1 (PAF-AHIb1) and the decreased activity of PAF-AH in type 1 diabetes (T1D) patients, we hypothesized that PAF-AHIb1 in Idd4.1 is a candidate gene. The PAF-AHIb1 gene in NOD mice was cloned and sequenced, and its expression and function were studied. No polymorphisms were detected in PAF-AHIb1 cDNA between NOD and B6 mice. The expression of PAF-AH Ib1 at the mRNA and protein levels was found to be similar in different tissues between NOD and B6 mice. PAF-AH activity does not differ in the pancreatic islets or spleen between NOD and B6 mice. Our findings suggest that PAF-AH Ib1 may not be a diabetes-susceptibility gene in the Idd4.1 sublocus.

AB - We recently mapped Idd4 to a 5.2 cM interval on chromosome 11 with two subloci, Idd4.1 and Idd4.2, in nonobese diabetic (NOD) mice. Based on the localization of platelet-activating factor acetylhydrolase Ib1 (PAF-AHIb1) and the decreased activity of PAF-AH in type 1 diabetes (T1D) patients, we hypothesized that PAF-AHIb1 in Idd4.1 is a candidate gene. The PAF-AHIb1 gene in NOD mice was cloned and sequenced, and its expression and function were studied. No polymorphisms were detected in PAF-AHIb1 cDNA between NOD and B6 mice. The expression of PAF-AH Ib1 at the mRNA and protein levels was found to be similar in different tissues between NOD and B6 mice. PAF-AH activity does not differ in the pancreatic islets or spleen between NOD and B6 mice. Our findings suggest that PAF-AH Ib1 may not be a diabetes-susceptibility gene in the Idd4.1 sublocus.

KW - Diabetes

KW - Genetic susceptibility

KW - Idd4

KW - Nonobese diabetic mice

KW - Platelet-activating factor acetylhydrolase Ib1 gene

UR - http://www.scopus.com/inward/record.url?scp=33845696030&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845696030&partnerID=8YFLogxK

U2 - 10.1196/annals.1375.022

DO - 10.1196/annals.1375.022

M3 - Conference contribution

C2 - 17130546

AN - SCOPUS:33845696030

SN - 1573316415

SN - 9781573316415

T3 - Annals of the New York Academy of Sciences

SP - 147

EP - 152

BT - Immunology of Diabetes IV

PB - Blackwell Publishing Inc.

ER -