Characterization of primary osteocyte-like cells from rat mandibles

Ibrahim El deeb Zakhary, Karl Wenger, Mohammed Elsayed Elsalanty, James Cray, Mohamed M.H. Sharawy, Regina L W Messer

Research output: Contribution to journalArticle

Abstract

Objective The mandible is continuously undergoing remodeling as a result of mechanobiologic factors, such as chewing forces, tooth loss, orthodontic forces, and periodontitis. The effects of mechanical stress and biologic signals in bone homeostasis have been the focus of many investigations. However, much of this research utilized osteocytes derived from long bones, but little is known about the mandible-derived osteocytes. This study tests a protocol to isolate and grow osteocytes from rat mandible. Study Design Rat mandibles were harvested, sectioned into small pieces, and subjected to a sequence chemical treatment and enzymatic digestion. The treated tissues were cultured for a few weeks while cells emerged. Cells were sorted by using the osteocyte marker podoplanin, an early marker for osteocyte differentiation. The cells were then characterized according to morphology, biochemical markers (osteocalcin, podoplanin, and sclerostin), and alkaline phosphatase activity and compared with an isotype cell line MLO-Y4 cells. Results The mandibular osteocytic cells had stellate shape and were positive for osteocalcin, podoplanin, and sclerostin and lower alkaline phosphatase activity compared with MLO-Y4 osteocyte-like cells. Conclusions The protocol to isolate osteocyte-like cells will allow the investigators to investigate the mechanobiologic differences in biomechanical response between these mandibular and long bone osteocyte-like cells under various conditions.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalOral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Volume123
Issue number1
DOIs
StatePublished - Jan 1 2017

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Osteocytes
Mandible
Osteocalcin
Bone and Bones
Alkaline Phosphatase
Tooth Loss
Mechanical Stress
Periodontitis
Mastication
Differentiation Antigens
Orthodontics
Digestion
Homeostasis
Biomarkers
Research Personnel
Cell Line

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Pathology and Forensic Medicine
  • Dentistry (miscellaneous)
  • Radiology Nuclear Medicine and imaging

Cite this

Characterization of primary osteocyte-like cells from rat mandibles. / El deeb Zakhary, Ibrahim; Wenger, Karl; Elsalanty, Mohammed Elsayed; Cray, James; Sharawy, Mohamed M.H.; Messer, Regina L W.

In: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, Vol. 123, No. 1, 01.01.2017, p. 37-43.

Research output: Contribution to journalArticle

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