Characterization of the antioxidant activity of aglycone and glycosylated derivatives of hesperetin

An in vitro and in vivo study

Verônica Trícoli De Souza, Élida Paula Dini De Franco, Maria Elisa Melo Branco De Araújo, Márcia Cristina Fernandes Messias, Fernanda Priviero, Alexandra C.H. Frankland Sawaya, Patricia De Oliveira Carvalho

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The flavonoids are mainly present in Citrus fruits as their glycosyl derivatives. This study was conducted comparing in vitro xanthine oxidase inhibitory activity of the aglycone hesperetin and its glycosylated forms (hesperidin and G-hesperidin) and their effects on the plasma lipid profile and the oxidative-antioxidative system (TBARS and antioxidant enzymes) in rats. The concentrations of the major conjugated metabolites in rat plasma after oral administration of these compounds were also determined. Wistar male rats were randomly assigned to three groups (n = 6) supplemented for 30 days with 1 mmol/kg body mass of hesperetin, hesperidin or G-hesperidin. Hesperetin was a stronger xanthine oxidase inhibitor (IC50 = 53 μM and Ki = 17.3 μM) than the glycosylate derivatives. Supplementation with the three compounds led to a lower (more favorable) atherogenic index, and an antioxidant preventive effect from the increase of hepatic superoxide dismutase was observed associated to HT supplementation, possibly because of the higher level of hesperetin-glucuronide in rat plasma.

Original languageEnglish (US)
Pages (from-to)80-87
Number of pages8
JournalJournal of Molecular Recognition
Volume29
Issue number2
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

Fingerprint

Hesperidin
Antioxidants
Xanthine Oxidase
Citrus
Glucuronides
Flavonoids
Inhibitory Concentration 50
Superoxide Dismutase
Oral Administration
Wistar Rats
Lipids
In Vitro Techniques
hesperetin
Liver
Enzymes

Keywords

  • bioavailability
  • hesperetin
  • lipid profile
  • oxidative-antioxidative system

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

De Souza, V. T., De Franco, É. P. D., De Araújo, M. E. M. B., Messias, M. C. F., Priviero, F., Frankland Sawaya, A. C. H., & De Oliveira Carvalho, P. (2016). Characterization of the antioxidant activity of aglycone and glycosylated derivatives of hesperetin: An in vitro and in vivo study. Journal of Molecular Recognition, 29(2), 80-87. https://doi.org/10.1002/jmr.2509

Characterization of the antioxidant activity of aglycone and glycosylated derivatives of hesperetin : An in vitro and in vivo study. / De Souza, Verônica Trícoli; De Franco, Élida Paula Dini; De Araújo, Maria Elisa Melo Branco; Messias, Márcia Cristina Fernandes; Priviero, Fernanda; Frankland Sawaya, Alexandra C.H.; De Oliveira Carvalho, Patricia.

In: Journal of Molecular Recognition, Vol. 29, No. 2, 01.02.2016, p. 80-87.

Research output: Contribution to journalArticle

De Souza, VT, De Franco, ÉPD, De Araújo, MEMB, Messias, MCF, Priviero, F, Frankland Sawaya, ACH & De Oliveira Carvalho, P 2016, 'Characterization of the antioxidant activity of aglycone and glycosylated derivatives of hesperetin: An in vitro and in vivo study', Journal of Molecular Recognition, vol. 29, no. 2, pp. 80-87. https://doi.org/10.1002/jmr.2509
De Souza, Verônica Trícoli ; De Franco, Élida Paula Dini ; De Araújo, Maria Elisa Melo Branco ; Messias, Márcia Cristina Fernandes ; Priviero, Fernanda ; Frankland Sawaya, Alexandra C.H. ; De Oliveira Carvalho, Patricia. / Characterization of the antioxidant activity of aglycone and glycosylated derivatives of hesperetin : An in vitro and in vivo study. In: Journal of Molecular Recognition. 2016 ; Vol. 29, No. 2. pp. 80-87.
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