Characterization of the main phase transition in 1,2-dipalmitoyl-phosphatidylcholine LUVs by 1H NMR

Anthony Kennedy; Peter J. Hmel; Joseph Seelbaugh; John G. Quiles; Rickey Hicks; Thomas J. Reid

doi:10.1081/LPR-120014759

Characterization of the main phase transition in 1,2-dipalmitoyl-phosphatidylcholine LUVs by ¹H NMR

Anthony Kennedy, Peter J. Hmel, Joseph Seelbaugh, John G. Quiles, Rickey Hicks, Thomas J. Reid

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The main phase transition (Tm) of 100nm large unilamellar vesicles (LUVs) of 1,2-dipalmitoylphosphatidylcholine (DPPC) was investigated using ¹HNMR (proton magnetic resonance) in deuterium oxide, and both DSC (differential scanning calorimetry) and IR (infrared) spectroscopy in water and deuterium oxide. The ability of ¹H NMR to determine Tm was demonstrated and the values obtained were in general agreement with those observed with DSC and IR. However, the temperature range of the transition observed by NMR was significantly broader than that observed with either DSC or IR. The effect of deuterium oxide on Tm was studied by comparing results obtained in water and deuterium oxide with DSC and IR. The results showed no significant difference in Tm or temperature range of transition determined in these solvents.

Original language	English (US)
Pages (from-to)	221-237
Number of pages	17
Journal	Journal of Liposome Research
Volume	12
Issue number	3
DOIs	https://doi.org/10.1081/LPR-120014759
State	Published - 2002

Keywords

Membrane
NMR
Phase transition

ASJC Scopus subject areas

Pharmaceutical Science

Access to Document

10.1081/LPR-120014759

Cite this

@article{c6000cc3a0554cd69f9257e0daf2df6d,

title = "Characterization of the main phase transition in 1,2-dipalmitoyl-phosphatidylcholine LUVs by 1H NMR",

abstract = "The main phase transition (Tm) of 100nm large unilamellar vesicles (LUVs) of 1,2-dipalmitoylphosphatidylcholine (DPPC) was investigated using 1HNMR (proton magnetic resonance) in deuterium oxide, and both DSC (differential scanning calorimetry) and IR (infrared) spectroscopy in water and deuterium oxide. The ability of 1H NMR to determine Tm was demonstrated and the values obtained were in general agreement with those observed with DSC and IR. However, the temperature range of the transition observed by NMR was significantly broader than that observed with either DSC or IR. The effect of deuterium oxide on Tm was studied by comparing results obtained in water and deuterium oxide with DSC and IR. The results showed no significant difference in Tm or temperature range of transition determined in these solvents.",

keywords = "Membrane, NMR, Phase transition",

author = "Anthony Kennedy and Hmel, {Peter J.} and Joseph Seelbaugh and Quiles, {John G.} and Rickey Hicks and Reid, {Thomas J.}",

year = "2002",

doi = "10.1081/LPR-120014759",

language = "English (US)",

volume = "12",

pages = "221--237",

journal = "Journal of Liposome Research",

issn = "0898-2104",

publisher = "Informa Healthcare",

number = "3",

}

TY - JOUR

T1 - Characterization of the main phase transition in 1,2-dipalmitoyl-phosphatidylcholine LUVs by 1H NMR

AU - Kennedy, Anthony

AU - Hmel, Peter J.

AU - Seelbaugh, Joseph

AU - Quiles, John G.

AU - Hicks, Rickey

AU - Reid, Thomas J.

PY - 2002

Y1 - 2002

N2 - The main phase transition (Tm) of 100nm large unilamellar vesicles (LUVs) of 1,2-dipalmitoylphosphatidylcholine (DPPC) was investigated using 1HNMR (proton magnetic resonance) in deuterium oxide, and both DSC (differential scanning calorimetry) and IR (infrared) spectroscopy in water and deuterium oxide. The ability of 1H NMR to determine Tm was demonstrated and the values obtained were in general agreement with those observed with DSC and IR. However, the temperature range of the transition observed by NMR was significantly broader than that observed with either DSC or IR. The effect of deuterium oxide on Tm was studied by comparing results obtained in water and deuterium oxide with DSC and IR. The results showed no significant difference in Tm or temperature range of transition determined in these solvents.

AB - The main phase transition (Tm) of 100nm large unilamellar vesicles (LUVs) of 1,2-dipalmitoylphosphatidylcholine (DPPC) was investigated using 1HNMR (proton magnetic resonance) in deuterium oxide, and both DSC (differential scanning calorimetry) and IR (infrared) spectroscopy in water and deuterium oxide. The ability of 1H NMR to determine Tm was demonstrated and the values obtained were in general agreement with those observed with DSC and IR. However, the temperature range of the transition observed by NMR was significantly broader than that observed with either DSC or IR. The effect of deuterium oxide on Tm was studied by comparing results obtained in water and deuterium oxide with DSC and IR. The results showed no significant difference in Tm or temperature range of transition determined in these solvents.

KW - Membrane

KW - NMR

KW - Phase transition

UR - http://www.scopus.com/inward/record.url?scp=0036443998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036443998&partnerID=8YFLogxK

U2 - 10.1081/LPR-120014759

DO - 10.1081/LPR-120014759

M3 - Article

C2 - 12604028

AN - SCOPUS:0036443998

SN - 0898-2104

VL - 12

SP - 221

EP - 237

JO - Journal of Liposome Research

JF - Journal of Liposome Research

IS - 3

ER -