Characterization of the Regulatory Function of the ICP22 Protein of Equine Herpesvirus Type 1

V. Roger Holden, Yuhe Zhao, Yeini Thompson, Gretchen B Caughman, Richard H. Smith, Dennis J. O'Callaghan

Research output: Contribution to journalArticle

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Abstract

The IR4 gene (inverted repeat gene 4) of equine herpesvirus type 1 (EHV-), the homolog of the herpes simplex virus type 1 ICP22 gene, is differentially expressed as a 1.4-kb early transcript and a 1.7-kb late transcript that encode a series of proteins that migrate between 42 to 47 kDa, localize to the nucleus of EHV-1-infected cells, and become packaged within EHV-1 virions (V. R. Holden, G. B. Caughman, Y. Zhao, R. N. Harry, and D. J. O'Callaghan, J. Virol. 68, 4329-4340, 1994). To assess the role of the IR4 protein in EHV-1 gene regulation, an IR4 expression vector was cotransfected with EHV-1 chimeric promoter-CAT reporter constructs and EHV-1 effector plasmids to determine the effects of the IR4 protein on the expression of immediate-early (IE), early, and late promoters. These studies revealed that the IR4 protein: (i) minimally trans-activates EHV-1 promoters, (ii) acts synergistically with the UL3 (ICP27) gene product to trans-activate the IE promoter, (iii) does not interfere with the trans-repression of the IE promoter by the IE protein, (iv) enhances transactivation of early promoters by the IE protein, (v) enhances the transactivation of both early and late promoters by the IE and UL3 proteins, and (vi) interacts synergistically with the IE protein to trans-activate the heterologous HSV-1 ICP4 promoter. These data suggest that the IR4 gene product plays a significant role in EHV-1 gene regulation.

Original languageEnglish (US)
Article number71344
Pages (from-to)273-282
Number of pages10
JournalVirology
Volume210
Issue number2
DOIs
StatePublished - Jul 10 1995

Fingerprint

Equid Herpesvirus 1
Immediate-Early Proteins
Proteins
Genes
Transcriptional Activation
Human Herpesvirus 1
Gene Expression Regulation
Virion
Plasmids

ASJC Scopus subject areas

  • Virology

Cite this

Holden, V. R., Zhao, Y., Thompson, Y., Caughman, G. B., Smith, R. H., & O'Callaghan, D. J. (1995). Characterization of the Regulatory Function of the ICP22 Protein of Equine Herpesvirus Type 1. Virology, 210(2), 273-282. [71344]. https://doi.org/10.1006/viro.1995.1344

Characterization of the Regulatory Function of the ICP22 Protein of Equine Herpesvirus Type 1. / Holden, V. Roger; Zhao, Yuhe; Thompson, Yeini; Caughman, Gretchen B; Smith, Richard H.; O'Callaghan, Dennis J.

In: Virology, Vol. 210, No. 2, 71344, 10.07.1995, p. 273-282.

Research output: Contribution to journalArticle

Holden, VR, Zhao, Y, Thompson, Y, Caughman, GB, Smith, RH & O'Callaghan, DJ 1995, 'Characterization of the Regulatory Function of the ICP22 Protein of Equine Herpesvirus Type 1', Virology, vol. 210, no. 2, 71344, pp. 273-282. https://doi.org/10.1006/viro.1995.1344
Holden, V. Roger ; Zhao, Yuhe ; Thompson, Yeini ; Caughman, Gretchen B ; Smith, Richard H. ; O'Callaghan, Dennis J. / Characterization of the Regulatory Function of the ICP22 Protein of Equine Herpesvirus Type 1. In: Virology. 1995 ; Vol. 210, No. 2. pp. 273-282.
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