Characterization of the regulatory functions of the equine herpesvirus 1 immediate-early gene product

Richard H. Smith, Gretchen B Caughman, Dennis J. O'Callaghan

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Use of the translation-inhibiting drug cycloheximide has indicated that the equine herpesvirus 1 (EHV-1) immediate-early (IE) gene, the sole EHV-1 IE gene, encodes a major viral regulatory protein since IE mRNA translation is a prerequisite for all further viral gene expression (W. L. Gray, R. P. Baumann, A. T. Robertson, G. B. Caughman, D. J. O'Callaghan, and J. Staczek, Virology 158:79-87, 1987). An EHV-1 IE gene expression vector (pSVIE) in combination with chimeric EHV-1 promoter-chloramphenicol acetyltransferase (CAT) reporter constructs was used in transient transfection assays to characterize the regulatory functions of the IE gene product. These experiments demonstrated that (i) the EHV-1 IE gene product is a bifunctional protein capable of both positive and negative modulation of gene expression; (ii) the IE gene product possesses an autoregulatory function which represses the IE promoter; (iii) IE autoregulation is dependent on IE promoter sequences mapping within positions -288 to +73 relative to the transcription initiation site (+1) of the IE gene; (iv) the IE gene product can independently activate the EHV-1 tk promoter (an early promoter) by as much as 60-fold; (v) two EHV-1 beta-gamma (leaky late) promoters, those of IR5 (gene 5 in the inverted repeat) and the glycoprotein D gene, demonstrate a requirement for both the IE gene product as well as a gene product encoded within the EHV-1 XbaI G fragment for significant activation; and (vi) the IE gene product is capable of activating heterologous viral promoters.

Original languageEnglish (US)
Pages (from-to)936-945
Number of pages10
JournalJournal of Virology
Volume66
Issue number2
StatePublished - Feb 1 1992

Fingerprint

Equid Herpesvirus 1
Equid herpesvirus 1
Immediate-Early Genes
promoter regions
genes
Gene Expression
gene expression
translation (genetics)
Viral Regulatory and Accessory Proteins
Genes
chloramphenicol acetyltransferase
Virology
Chloramphenicol O-Acetyltransferase
autoregulation
Viral Genes
virology
Transcription Initiation Site
Protein Biosynthesis
Cycloheximide
viral proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of the regulatory functions of the equine herpesvirus 1 immediate-early gene product. / Smith, Richard H.; Caughman, Gretchen B; O'Callaghan, Dennis J.

In: Journal of Virology, Vol. 66, No. 2, 01.02.1992, p. 936-945.

Research output: Contribution to journalArticle

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