Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo

J. Hebling, D. H. Pashley, L. Tjäderhane, F. R. Tay

Research output: Contribution to journalArticle

348 Citations (Scopus)

Abstract

The recent paradigm that endogenous collagenolytic and gelatinolytic activities derived from acid-etched dentin result in degradation of hybrid layers requires in vivo validation. This study tested the null hypothesis that there is no difference between the degradation of dentin bonded with an etch-and-rinse adhesive and that in conjunction with chlorhexidine, an MMP inhibitor, applied after phosphoric-acid-etching. Contralateral pairs of bonded Class I restorations in primary molars of clinical subjects were retrieved after a six-month period of intra-oral functioning and processed for transmission electron microscopy. Hybrid layers from the chlorhexidine-treated teeth exhibited normal structural integrity of the collagen network. Conversely, abnormal hybrid layers were seen in the control teeth, with progressive disintegration of the fibrillar network, to the extent that it was beyond detection by collagen staining. Self-destruction of collagen matrices occurs rapidly in resin-infiltrated dentin in vivo and may be arrested with the use of chlorhexidine as an MMP inhibitor.

Original languageEnglish (US)
Pages (from-to)741-746
Number of pages6
JournalJournal of Dental Research
Volume84
Issue number8
DOIs
StatePublished - Aug 1 2005

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Chlorhexidine
Dentin
Matrix Metalloproteinase Inhibitors
Collagen
Tooth
Transmission Electron Microscopy
Adhesives
Staining and Labeling
Acids

Keywords

  • Collagenolysis
  • Gelatinolysis
  • Hybrid layer
  • MMPs
  • Primary dentin

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. / Hebling, J.; Pashley, D. H.; Tjäderhane, L.; Tay, F. R.

In: Journal of Dental Research, Vol. 84, No. 8, 01.08.2005, p. 741-746.

Research output: Contribution to journalArticle

Hebling, J. ; Pashley, D. H. ; Tjäderhane, L. ; Tay, F. R. / Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. In: Journal of Dental Research. 2005 ; Vol. 84, No. 8. pp. 741-746.
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