Chlorhexidine preserves dentin bond in vitro

M. R.O. Carrilho, R. M. Carvalho, M. F. De Goes, V. Di Hipólito, S. Geraldeli, Franklin Chi Meng Tay, David Henry Pashley, L. Tjäderhane

Research output: Contribution to journalArticle

262 Citations (Scopus)

Abstract

Loss of hybrid layer integrity compromises resindentin bond stability. Matrix metalloproteinases (MMPs) may be partially responsible for hybrid layer degradation. Since chlorhexidine inhibits MMPs, we hypothesized that chlorhexidine would decelerate the loss of resin-dentin bonds. Class I preparations in extracted third molars were sectioned into two halves. One half was customarily restored (etch-and-rinse adhesive/resin composite), and the other was treated with 2% chlorhexidine after being acid-etched before restoration. Specimens were stored in artificial saliva with/without protease inhibitors. Microtensile bond strengths and failure mode distribution under SEM were analyzed immediately after specimens' preparation and 6 months later. With chlorhexidine, significantly better preservation of bond strength was observed after 6 months; protease inhibitors in the storage medium had no effect. Failure analysis showed significantly less failure in the hybrid layer with chlorhexidine, compared with controls after 6 months. In conclusion, this in vitro study suggests that chlorhexidine might be useful for the preservation of dentin bond strength.

Original languageEnglish (US)
Pages (from-to)90-94
Number of pages5
JournalJournal of Dental Research
Volume86
Issue number1
DOIs
StatePublished - Jan 1 2007

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Chlorhexidine
Dentin
Protease Inhibitors
Matrix Metalloproteinases
Artificial Saliva
Third Molar
Composite Resins
Adhesives
In Vitro Techniques
Acids

Keywords

  • Adhesive
  • Hybrid layer
  • Matrix metalloproteinase
  • Microtensile
  • Tooth

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Carrilho, M. R. O., Carvalho, R. M., De Goes, M. F., Di Hipólito, V., Geraldeli, S., Tay, F. C. M., ... Tjäderhane, L. (2007). Chlorhexidine preserves dentin bond in vitro. Journal of Dental Research, 86(1), 90-94. https://doi.org/10.1177/154405910708600115

Chlorhexidine preserves dentin bond in vitro. / Carrilho, M. R.O.; Carvalho, R. M.; De Goes, M. F.; Di Hipólito, V.; Geraldeli, S.; Tay, Franklin Chi Meng; Pashley, David Henry; Tjäderhane, L.

In: Journal of Dental Research, Vol. 86, No. 1, 01.01.2007, p. 90-94.

Research output: Contribution to journalArticle

Carrilho, MRO, Carvalho, RM, De Goes, MF, Di Hipólito, V, Geraldeli, S, Tay, FCM, Pashley, DH & Tjäderhane, L 2007, 'Chlorhexidine preserves dentin bond in vitro', Journal of Dental Research, vol. 86, no. 1, pp. 90-94. https://doi.org/10.1177/154405910708600115
Carrilho MRO, Carvalho RM, De Goes MF, Di Hipólito V, Geraldeli S, Tay FCM et al. Chlorhexidine preserves dentin bond in vitro. Journal of Dental Research. 2007 Jan 1;86(1):90-94. https://doi.org/10.1177/154405910708600115
Carrilho, M. R.O. ; Carvalho, R. M. ; De Goes, M. F. ; Di Hipólito, V. ; Geraldeli, S. ; Tay, Franklin Chi Meng ; Pashley, David Henry ; Tjäderhane, L. / Chlorhexidine preserves dentin bond in vitro. In: Journal of Dental Research. 2007 ; Vol. 86, No. 1. pp. 90-94.
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