Choroid plexus ependymal cells host neural progenitor cells in the rat

Yutaka Itokazu, Masaaki Kitada, Mari Dezawa, Akira Mizoguchi, Naoya Matsumoto, Akira Shimizu, Chizuka Ide

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We previously demonstrated that choroid plexus epithelial (modified ependymal) cells (CPECs) differentiated into astrocytes after grafting into the spinal cord. In the present study, we examined whether CPECs from rats at postnatal 1 day (P1), 7 day (P7), and 8 weeks (P8W) can function as neural progenitor cells that give rise to neurons and glial cells. Cell spheres were produced in cultures of whole tissue of the choroid plexus from the fourth ventricle of rats at each postnatal period, β-tubulin class III (Tuj-1), glial fibrillary acid protein (GFAP)-, and 04-positive cells differentiated from cell spheres in the differentiation medium. We produced a monoclonal antibody 3E6 specifically labeling microvilli of CPECs. Using this monoclonal antibody, CPECs were isolated from the choroid plexus of P8W rats by cell sorter (FACS). Immunocytochemistry confirmed that there was no contamination from fibroblasts, endothelial cells, macrophages, or Schwann cells in the FACS-isolated 3E6-labeled cells. Cell spheres formed in the cultures of these 3E6-labeled CPECs. After expansion, these cell spheres gave rise to Tuj-1- (5%), GFAP- (45%), and 04-positive cells (0.16%). The remaining cells (45%) were unlabeled neural or glial markers. Some CPECs of the P8W rat were immunohistochemically stained with lineage-associated markers of Musashi-1 and epidermal growth factor-receptor (EGF-R). In addition, infusion of EGF or fibroblast growth factor-2 (FGF2) into the ventricle increased the number of bromodeoxyuridine (BrdU)-positive cells among CPECs from 0.03% (untreated) to 1.14% (38-fold, EGF) and 1.03% (35-fold, FGF2), respectively. These findings indicate that neural progenitor cells exist among CPECs in the rat.

Original languageEnglish (US)
Pages (from-to)32-42
Number of pages11
JournalGlia
Volume53
Issue number1
DOIs
StatePublished - Jan 1 2006

Fingerprint

Choroid Plexus
Stem Cells
Glial Fibrillary Acidic Protein
Fibroblast Growth Factor 2
Epidermal Growth Factor
Neuroglia
Monoclonal Antibodies
Fourth Ventricle
Schwann Cells
Bromodeoxyuridine
Tubulin
Microvilli
Epidermal Growth Factor Receptor
Astrocytes
Spinal Cord
Endothelial Cells
Fibroblasts
Immunohistochemistry
Macrophages

Keywords

  • Cell lineage
  • FACS
  • Immunoelectron microscopy
  • Immunohistochemistry
  • Monoclonal antibody

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Itokazu, Y., Kitada, M., Dezawa, M., Mizoguchi, A., Matsumoto, N., Shimizu, A., & Ide, C. (2006). Choroid plexus ependymal cells host neural progenitor cells in the rat. Glia, 53(1), 32-42. https://doi.org/10.1002/glia.20255

Choroid plexus ependymal cells host neural progenitor cells in the rat. / Itokazu, Yutaka; Kitada, Masaaki; Dezawa, Mari; Mizoguchi, Akira; Matsumoto, Naoya; Shimizu, Akira; Ide, Chizuka.

In: Glia, Vol. 53, No. 1, 01.01.2006, p. 32-42.

Research output: Contribution to journalArticle

Itokazu, Y, Kitada, M, Dezawa, M, Mizoguchi, A, Matsumoto, N, Shimizu, A & Ide, C 2006, 'Choroid plexus ependymal cells host neural progenitor cells in the rat', Glia, vol. 53, no. 1, pp. 32-42. https://doi.org/10.1002/glia.20255
Itokazu Y, Kitada M, Dezawa M, Mizoguchi A, Matsumoto N, Shimizu A et al. Choroid plexus ependymal cells host neural progenitor cells in the rat. Glia. 2006 Jan 1;53(1):32-42. https://doi.org/10.1002/glia.20255
Itokazu, Yutaka ; Kitada, Masaaki ; Dezawa, Mari ; Mizoguchi, Akira ; Matsumoto, Naoya ; Shimizu, Akira ; Ide, Chizuka. / Choroid plexus ependymal cells host neural progenitor cells in the rat. In: Glia. 2006 ; Vol. 53, No. 1. pp. 32-42.
@article{1aac3edec24a457484c28b8958f627ed,
title = "Choroid plexus ependymal cells host neural progenitor cells in the rat",
abstract = "We previously demonstrated that choroid plexus epithelial (modified ependymal) cells (CPECs) differentiated into astrocytes after grafting into the spinal cord. In the present study, we examined whether CPECs from rats at postnatal 1 day (P1), 7 day (P7), and 8 weeks (P8W) can function as neural progenitor cells that give rise to neurons and glial cells. Cell spheres were produced in cultures of whole tissue of the choroid plexus from the fourth ventricle of rats at each postnatal period, β-tubulin class III (Tuj-1), glial fibrillary acid protein (GFAP)-, and 04-positive cells differentiated from cell spheres in the differentiation medium. We produced a monoclonal antibody 3E6 specifically labeling microvilli of CPECs. Using this monoclonal antibody, CPECs were isolated from the choroid plexus of P8W rats by cell sorter (FACS). Immunocytochemistry confirmed that there was no contamination from fibroblasts, endothelial cells, macrophages, or Schwann cells in the FACS-isolated 3E6-labeled cells. Cell spheres formed in the cultures of these 3E6-labeled CPECs. After expansion, these cell spheres gave rise to Tuj-1- (5{\%}), GFAP- (45{\%}), and 04-positive cells (0.16{\%}). The remaining cells (45{\%}) were unlabeled neural or glial markers. Some CPECs of the P8W rat were immunohistochemically stained with lineage-associated markers of Musashi-1 and epidermal growth factor-receptor (EGF-R). In addition, infusion of EGF or fibroblast growth factor-2 (FGF2) into the ventricle increased the number of bromodeoxyuridine (BrdU)-positive cells among CPECs from 0.03{\%} (untreated) to 1.14{\%} (38-fold, EGF) and 1.03{\%} (35-fold, FGF2), respectively. These findings indicate that neural progenitor cells exist among CPECs in the rat.",
keywords = "Cell lineage, FACS, Immunoelectron microscopy, Immunohistochemistry, Monoclonal antibody",
author = "Yutaka Itokazu and Masaaki Kitada and Mari Dezawa and Akira Mizoguchi and Naoya Matsumoto and Akira Shimizu and Chizuka Ide",
year = "2006",
month = "1",
day = "1",
doi = "10.1002/glia.20255",
language = "English (US)",
volume = "53",
pages = "32--42",
journal = "GLIA",
issn = "0894-1491",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Choroid plexus ependymal cells host neural progenitor cells in the rat

AU - Itokazu, Yutaka

AU - Kitada, Masaaki

AU - Dezawa, Mari

AU - Mizoguchi, Akira

AU - Matsumoto, Naoya

AU - Shimizu, Akira

AU - Ide, Chizuka

PY - 2006/1/1

Y1 - 2006/1/1

N2 - We previously demonstrated that choroid plexus epithelial (modified ependymal) cells (CPECs) differentiated into astrocytes after grafting into the spinal cord. In the present study, we examined whether CPECs from rats at postnatal 1 day (P1), 7 day (P7), and 8 weeks (P8W) can function as neural progenitor cells that give rise to neurons and glial cells. Cell spheres were produced in cultures of whole tissue of the choroid plexus from the fourth ventricle of rats at each postnatal period, β-tubulin class III (Tuj-1), glial fibrillary acid protein (GFAP)-, and 04-positive cells differentiated from cell spheres in the differentiation medium. We produced a monoclonal antibody 3E6 specifically labeling microvilli of CPECs. Using this monoclonal antibody, CPECs were isolated from the choroid plexus of P8W rats by cell sorter (FACS). Immunocytochemistry confirmed that there was no contamination from fibroblasts, endothelial cells, macrophages, or Schwann cells in the FACS-isolated 3E6-labeled cells. Cell spheres formed in the cultures of these 3E6-labeled CPECs. After expansion, these cell spheres gave rise to Tuj-1- (5%), GFAP- (45%), and 04-positive cells (0.16%). The remaining cells (45%) were unlabeled neural or glial markers. Some CPECs of the P8W rat were immunohistochemically stained with lineage-associated markers of Musashi-1 and epidermal growth factor-receptor (EGF-R). In addition, infusion of EGF or fibroblast growth factor-2 (FGF2) into the ventricle increased the number of bromodeoxyuridine (BrdU)-positive cells among CPECs from 0.03% (untreated) to 1.14% (38-fold, EGF) and 1.03% (35-fold, FGF2), respectively. These findings indicate that neural progenitor cells exist among CPECs in the rat.

AB - We previously demonstrated that choroid plexus epithelial (modified ependymal) cells (CPECs) differentiated into astrocytes after grafting into the spinal cord. In the present study, we examined whether CPECs from rats at postnatal 1 day (P1), 7 day (P7), and 8 weeks (P8W) can function as neural progenitor cells that give rise to neurons and glial cells. Cell spheres were produced in cultures of whole tissue of the choroid plexus from the fourth ventricle of rats at each postnatal period, β-tubulin class III (Tuj-1), glial fibrillary acid protein (GFAP)-, and 04-positive cells differentiated from cell spheres in the differentiation medium. We produced a monoclonal antibody 3E6 specifically labeling microvilli of CPECs. Using this monoclonal antibody, CPECs were isolated from the choroid plexus of P8W rats by cell sorter (FACS). Immunocytochemistry confirmed that there was no contamination from fibroblasts, endothelial cells, macrophages, or Schwann cells in the FACS-isolated 3E6-labeled cells. Cell spheres formed in the cultures of these 3E6-labeled CPECs. After expansion, these cell spheres gave rise to Tuj-1- (5%), GFAP- (45%), and 04-positive cells (0.16%). The remaining cells (45%) were unlabeled neural or glial markers. Some CPECs of the P8W rat were immunohistochemically stained with lineage-associated markers of Musashi-1 and epidermal growth factor-receptor (EGF-R). In addition, infusion of EGF or fibroblast growth factor-2 (FGF2) into the ventricle increased the number of bromodeoxyuridine (BrdU)-positive cells among CPECs from 0.03% (untreated) to 1.14% (38-fold, EGF) and 1.03% (35-fold, FGF2), respectively. These findings indicate that neural progenitor cells exist among CPECs in the rat.

KW - Cell lineage

KW - FACS

KW - Immunoelectron microscopy

KW - Immunohistochemistry

KW - Monoclonal antibody

UR - http://www.scopus.com/inward/record.url?scp=30444460613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=30444460613&partnerID=8YFLogxK

U2 - 10.1002/glia.20255

DO - 10.1002/glia.20255

M3 - Article

C2 - 16158416

AN - SCOPUS:30444460613

VL - 53

SP - 32

EP - 42

JO - GLIA

JF - GLIA

SN - 0894-1491

IS - 1

ER -