Chromatin remodeling and activation of chromosomal DNA replication by an acidic transcriptional activation domain from BRCA1

Yan Fen Hu, Zhong Lin Hao, Rong Li

Research output: Contribution to journalArticle

102 Scopus citations

Abstract

An increasing number of transcription factors have been shown to activate DNA replication. However, the underlying mechanism remains to be elucidated. Here it is shown that when tethered to a cellular replication origin, the acidic transcriptional activation domain of the breast cancer protein BRCA1 alters the local chromatin structure and stimulates chromosomal DNA replication. Cancer-predisposing mutations in BRCA1 that abolish transcriptional activation also prevent chromatin remodeling and activation of replication. Chromatin remodeling occurs even in the absence of a functional replication origin. Thus, increasing chromatin accessibility may be an important mechanism used by transcription factors to facilitate multiple nuclear processes.

Original languageEnglish (US)
Pages (from-to)637-642
Number of pages6
JournalGenes and Development
Volume13
Issue number6
DOIs
StatePublished - Mar 15 1999

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Keywords

  • ARS1
  • BRCA1
  • Chromatin remodeling
  • Replication
  • Transcription factors
  • Yeast

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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