Chronic ANG II infusion induces sex-specific increases in renal T cells in Sprague-Dawley rats

Margaret A. Zimmerman, Babak Baban, Ashlee J. Tipton, Paul M O'Connor, Jennifer C Sullivan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Recent studies suggest that sex of the animal and T cell impact ANG II hypertension in Rag_/_ mice, with females being protected relative to males. This study tested the hypothesis that ANG II results in greater increases in proinflammatory T cells and cytokines in males than in females. Male and female Sprague-Dawley (SD) rats, aged 12 wk, were treated with vehicle or ANG II (200 ng·kg-1·min-1) for 2 wk. Renal CD4+ T cells and Tregs were comparable between vehicletreated males and females, although males expressed more Th17 and IL-17+ T cells and fewer IL-10+ T cells than females. ANG II resulted in greater increases in CD4+ T cells, Th17 cells, and IL-17+cells in males; Tregs increased only in females. We previously showed that ANG (1–7) antagonizes ANG II-induced increases in blood pressure in females and ANG (1–7) has been suggested to be anti-inflammatory. Renal ANG (1–7) levels were greater in female SD at baseline and following ANG II infusion. Additional rats were treated with ANG II plus the ANG (1–7)-mas receptor antagonist A-779 (48μg·kg-1·h-1) to test the hypothesis that greater ANG (1–7) in females results in more Tregs relative to males. Inhibition of ANG (1–7) did not alter renal T cells in either sex. In conclusion, ANG II induces a sex-specific effect on the renal T cell profile. Males have greater increases in proinflammatory T cells, and females have greater increases in anti-inflammatory Tregs; however, sex differences in the renal T cell profile are not mediated by ANG (1–7).

Original languageEnglish (US)
Pages (from-to)F706-F712
JournalAmerican Journal of Physiology - Renal Physiology
Volume308
Issue number7
DOIs
StatePublished - Jan 1 2015

Fingerprint

Sprague Dawley Rats
T-Lymphocytes
Kidney
Interleukin-17
7-Ala-angiotensin (1-7)
Anti-Inflammatory Agents
Th17 Cells
Sex Characteristics
Interleukin-10
Cytokines
Blood Pressure
Hypertension

Keywords

  • ANG (1–7)
  • Adaptive immune system
  • Cytokines
  • Gender
  • Sex differences
  • Sprague-Dawley

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Chronic ANG II infusion induces sex-specific increases in renal T cells in Sprague-Dawley rats. / Zimmerman, Margaret A.; Baban, Babak; Tipton, Ashlee J.; O'Connor, Paul M; Sullivan, Jennifer C.

In: American Journal of Physiology - Renal Physiology, Vol. 308, No. 7, 01.01.2015, p. F706-F712.

Research output: Contribution to journalArticle

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abstract = "Recent studies suggest that sex of the animal and T cell impact ANG II hypertension in Rag_/_ mice, with females being protected relative to males. This study tested the hypothesis that ANG II results in greater increases in proinflammatory T cells and cytokines in males than in females. Male and female Sprague-Dawley (SD) rats, aged 12 wk, were treated with vehicle or ANG II (200 ng·kg-1·min-1) for 2 wk. Renal CD4+ T cells and Tregs were comparable between vehicletreated males and females, although males expressed more Th17 and IL-17+ T cells and fewer IL-10+ T cells than females. ANG II resulted in greater increases in CD4+ T cells, Th17 cells, and IL-17+cells in males; Tregs increased only in females. We previously showed that ANG (1–7) antagonizes ANG II-induced increases in blood pressure in females and ANG (1–7) has been suggested to be anti-inflammatory. Renal ANG (1–7) levels were greater in female SD at baseline and following ANG II infusion. Additional rats were treated with ANG II plus the ANG (1–7)-mas receptor antagonist A-779 (48μg·kg-1·h-1) to test the hypothesis that greater ANG (1–7) in females results in more Tregs relative to males. Inhibition of ANG (1–7) did not alter renal T cells in either sex. In conclusion, ANG II induces a sex-specific effect on the renal T cell profile. Males have greater increases in proinflammatory T cells, and females have greater increases in anti-inflammatory Tregs; however, sex differences in the renal T cell profile are not mediated by ANG (1–7).",
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