Chronic, low-level exposure to diisopropylfluorophosphate causes protracted impairment of spatial navigation learning

Mark A. Prendergast, Alvin V Terry, Jerry J. Buccafusco

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Chronic, low-level exposure to cholinesterase inhibitor organophosphate (OP) insecticides or chemical warfare agents produces abnormalities in CNS acetylcholine (ACh) function, and in humans, may be associated with impaired cognitive function well after withdrawal from such exposure. The purpose of the present study was to identify the severity of impairment in spatial learning of rats following protracted withdrawal from chronic, low-level exposure to the OP agent diisopropylfluorophosphate (DFP). Assessment of spatial learning began either 3 or 17 days after completion of a 14-day DFP treatment regimen (50, 250, or 500 μg/kg). During the 14-day treatment regimen, spontaneous activity and olfactory behaviors were suppressed, effects which subsided with repeated exposure to the 250 μg/kg dose regimen. In contrast, both behaviors were stimulated by exposure to the 50 μg/kg dose regimen, as was body weight gain. Performance of the spatial test of working memory was impaired for up to 21 days after withdrawal from treatment with a 250 μg/kg dose of DFP. AChE activity in the frontal cortex and hippocampus was suppressed to 42.58% and 50.35% of control levels, respectively, 3 days after completion of the DFP (250 μg/kg) treatment regimen. By 7 days after withdrawal from treatment, AChE activity in the cortex and hippocampus had recovered to 81.87% and 64.61% of control levels, respectively. These levels represent increases in activity of 39.29% and 14.26% in these regions, as compared to AChE activity 3 days after DFP withdrawal. By 21 days after withdrawal from treatment, AChE in both brain regions had recovered to levels similar to those of controls. Chronic, low-level OP exposure, therefore, produces protracted impairment of working memory after drug withdrawal that is not associated with continued suppression of AChE activity. This impairment may, however, be associated with a decreased rate of AChE recovery in the hippocampus, relative to the cortex. This decreased rate of enzyme recovery may contribute to hippocampal toxicity underlying protracted impairment of working memory.

Original languageEnglish (US)
Pages (from-to)183-191
Number of pages9
JournalPsychopharmacology
Volume129
Issue number2
DOIs
StatePublished - Jan 24 1997

Fingerprint

Isoflurophate
Organophosphates
Short-Term Memory
Hippocampus
Chemical Warfare Agents
Cholinesterase Inhibitors
Frontal Lobe
Insecticides
Cognition
Acetylcholine
Weight Gain
Body Weight
Spatial Learning
Spatial Navigation
Brain
Enzymes
Pharmaceutical Preparations

Keywords

  • DFP
  • acetylcholinesterase
  • body weight
  • hippocampus
  • locomotion
  • olfaction
  • organophosphate
  • spatial learning

ASJC Scopus subject areas

  • Pharmacology

Cite this

Chronic, low-level exposure to diisopropylfluorophosphate causes protracted impairment of spatial navigation learning. / Prendergast, Mark A.; Terry, Alvin V; Buccafusco, Jerry J.

In: Psychopharmacology, Vol. 129, No. 2, 24.01.1997, p. 183-191.

Research output: Contribution to journalArticle

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abstract = "Chronic, low-level exposure to cholinesterase inhibitor organophosphate (OP) insecticides or chemical warfare agents produces abnormalities in CNS acetylcholine (ACh) function, and in humans, may be associated with impaired cognitive function well after withdrawal from such exposure. The purpose of the present study was to identify the severity of impairment in spatial learning of rats following protracted withdrawal from chronic, low-level exposure to the OP agent diisopropylfluorophosphate (DFP). Assessment of spatial learning began either 3 or 17 days after completion of a 14-day DFP treatment regimen (50, 250, or 500 μg/kg). During the 14-day treatment regimen, spontaneous activity and olfactory behaviors were suppressed, effects which subsided with repeated exposure to the 250 μg/kg dose regimen. In contrast, both behaviors were stimulated by exposure to the 50 μg/kg dose regimen, as was body weight gain. Performance of the spatial test of working memory was impaired for up to 21 days after withdrawal from treatment with a 250 μg/kg dose of DFP. AChE activity in the frontal cortex and hippocampus was suppressed to 42.58{\%} and 50.35{\%} of control levels, respectively, 3 days after completion of the DFP (250 μg/kg) treatment regimen. By 7 days after withdrawal from treatment, AChE activity in the cortex and hippocampus had recovered to 81.87{\%} and 64.61{\%} of control levels, respectively. These levels represent increases in activity of 39.29{\%} and 14.26{\%} in these regions, as compared to AChE activity 3 days after DFP withdrawal. By 21 days after withdrawal from treatment, AChE in both brain regions had recovered to levels similar to those of controls. Chronic, low-level OP exposure, therefore, produces protracted impairment of working memory after drug withdrawal that is not associated with continued suppression of AChE activity. This impairment may, however, be associated with a decreased rate of AChE recovery in the hippocampus, relative to the cortex. This decreased rate of enzyme recovery may contribute to hippocampal toxicity underlying protracted impairment of working memory.",
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