Chz1, a Nuclear Chaperone for Histone H2AZ

Ed Luk, Ngoc Diep Vu, Kem Patteson, Gaku Mizuguchi, Wei Hua Wu, Anand Ranjan, Jonathon Backus, Subhojit Sen, Marc Lewis, Yawen Bai, Carl Wu

    Research output: Contribution to journalArticlepeer-review

    121 Scopus citations

    Abstract

    The histone variant H2AZ marks nucleosomes flanking the promoters of most genes of budding yeast. The incorporation of H2AZ into chromatin is dependent on the SWR1 complex, which catalyses the replacement of conventional histone H2A with H2AZ. In cells, the pool of unincorporated histone H2AZ has previously been found in association with Nap1, a chaperone for conventional histone H2A-H2B. Here, we report the discovery of Chz1, a histone chaperone that has preference for H2AZ and can also deliver a source of the histone variant for SWR1-dependent histone replacement. Bacterially expressed Chz1 forms a heterotrimer with H2AZ-H2B, stabilizing the association of the histone dimer. We have identified a conserved motif important for histone variant recognition within the H2AZ-interacting domain of Chz1. The presence of this motif in other metazoan proteins suggests that H2AZ-specific chaperones may be widely conserved.

    Original languageEnglish (US)
    Pages (from-to)357-368
    Number of pages12
    JournalMolecular Cell
    Volume25
    Issue number3
    DOIs
    StatePublished - Feb 9 2007

    Keywords

    • DNA

    ASJC Scopus subject areas

    • Molecular Biology
    • Cell Biology

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