Cigarette smoke extract inhibits angiogenesis of pulmonary artery endothelial cells: The role of calpain

Yunchao Su, Wengang Cao, Zhaosheng Han, Edward R. Block

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Angiogenesis is an integral part of both the pulmonary inflammatory response to chronic exposure to cigarette smoke and the lung tissue remodeling associated with cigarette smoke-induced chronic obstructive pulmonary disease (COPD). To investigate the role of angiogenesis in the pathogenesis of COPD, we evaluated the effect of cigarette smoke extract (CSE) on angiogenesis of pulmonary artery endothelial cells (PAEC). Incubation of PAEC with 2.5-10% CSE resulted in a dose-dependent inhibition of endothelial monolayer wound repair. CSE also caused inhibition of tube formation on Matrigel, migration in a Boyden chamber, and proliferation of PAEC. Because calpain, a family of calcium-dependent intracellular proteases, mediates cytoskeletal signaling in endothelial motility, we explored the role of calpain in the CSE-induced inhibition of endothelial angiogenesis. Incubation of CSE resulted in a dose-dependent decrease in calpain activity. Calpain inhibitor-1, a specific inhibitor of calpain, potentiates inhibitory effect of CSE on the endothelial monolayer wound repair, tube formation, cell migration, and cell proliferation. Transfection of PAEC with antisense oligodeoxyribonucleotides of calpastatin, the major endogenous calpain inhibitor, prevented CSE-induced increase in calpastatin protein content and CSE-induced decreases in calpain activity. It also prevented CSE-induced decreases in monolayer wound repair, tube formation, and migration. These results suggest that CSE attenuates angiogenesis of PAEC and the mechanism involves inhibition of calpain. Impaired angiogenesis may impede the repair process in the lungs of cigarette smokers and contribute to the altered structural remodeling observed in the lungs of patients with cigarette smoke-related COPD.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume287
Issue number4 31-4
DOIs
StatePublished - Oct 1 2004
Externally publishedYes

Fingerprint

Calpain
Smoke
Tobacco Products
Pulmonary Artery
Endothelial Cells
Chronic Obstructive Pulmonary Disease
Lung
Wounds and Injuries
Antisense Oligodeoxyribonucleotides
Cell Movement
Transfection

Keywords

  • Endothelium
  • Tobacco

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Cigarette smoke extract inhibits angiogenesis of pulmonary artery endothelial cells : The role of calpain. / Su, Yunchao; Cao, Wengang; Han, Zhaosheng; Block, Edward R.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 287, No. 4 31-4, 01.10.2004.

Research output: Contribution to journalArticle

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