Cisplatin-induced nephrotoxicity is mediated by tumor necrosis factor-α produced by renal parenchymal cells

B. Zhang, Ganesan Ramesh, C. C. Norbury, W. B. Reeves

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

Cisplatin is a chemotherapeutic agent that induces tumor necrosis factor-α (TNF-α) production in many cell types with unfortunate renal toxicity. We sought to determine the contributions of renal parenchymal cells and bone marrow-derived immune cells to the pathogenesis of cisplatin-induced renal injury in vivo. To do this we created chimeric mice in which the bone marrow was ablated and replaced with donor bone marrow cells from wild-type or from TNF-α knockout mice. Six weeks after reconstitution, the chimeric mice were treated with cisplatin and renal structural and functional parameters were measured. Chimeras with kidneys of wild-type animals all developed significant renal failure after 72 h of cisplatin treatment regardless of the immune cell source. Chimeras with kidneys of TNF-α knockout mice showed significantly less renal dysfunction (blood urea nitrogen, serum creatinine, and glomerular filtration rate), renal histologic injury, and serum TNF-α levels; again regardless of the immune cell source. Urinary excretion of several proinflammatory cytokines was lower in the wild-type bone marrow-knockout kidney chimera mouse than in wild-type background mice. Our results indicate that a substantial portion of circulating and urinary TNF-α is derived from nonimmune cells after cisplatin administration. We conclude that the production of TNF-α by renal parenchymal cells, rather than by bone marrow-derived infiltrating immune cells, is responsible for cisplatin-induced nephrotoxicity.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalKidney International
Volume72
Issue number1
DOIs
StatePublished - Jul 1 2007

Fingerprint

Cisplatin
Tumor Necrosis Factor-alpha
Kidney
Bone Marrow Cells
Knockout Mice
Bone Marrow
Wild Animals
Blood Urea Nitrogen
Wounds and Injuries
Serum
Glomerular Filtration Rate
Renal Insufficiency
Creatinine
Cytokines

Keywords

  • Acute renal failure
  • Chimeric mice
  • Cytokines
  • Inflammation

ASJC Scopus subject areas

  • Nephrology

Cite this

Cisplatin-induced nephrotoxicity is mediated by tumor necrosis factor-α produced by renal parenchymal cells. / Zhang, B.; Ramesh, Ganesan; Norbury, C. C.; Reeves, W. B.

In: Kidney International, Vol. 72, No. 1, 01.07.2007, p. 37-44.

Research output: Contribution to journalArticle

Zhang, B. ; Ramesh, Ganesan ; Norbury, C. C. ; Reeves, W. B. / Cisplatin-induced nephrotoxicity is mediated by tumor necrosis factor-α produced by renal parenchymal cells. In: Kidney International. 2007 ; Vol. 72, No. 1. pp. 37-44.
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