Clinical benefits of oral irrigation for periodontitis are related to reduction of pro-inflammatory cytokine levels and plaque

Christopher W. Cutler, Thomas W. Stanford, Celeste Abraham, Robert A. Cederberg, Thomas J. Boardman, Candy Ross

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Background: Although a growing body of evidence indicates that oral irrigation with water has therapeutic benefits in periodontitis, the mechanisms of action have not been elucidated. The aims of this study were: (1) to analyze the effects of oral irrigation (Water Pik® Oral Irrigator) on the clinical signs of adult periodontitis (AP) and on the levels of interleukin-1β (IL-β), prostaglandin-E2 (PGE2), interleukin-10 (IL-10) and interferon-γ (IFN-γ) in GCF, and (2) to analyze the influence of the periodontitis-related IL-1 genotype (IL-1GT) on these variables. Method: A single-center, blinded study in otherwise healthy humans (n=52) with localized mild to moderate AP was carried out, using the following groups: group A (n=12), no oral hygiene for 14 days; group B (n=20), routine oral hygiene (ROH) for 14 days; group C (n=20), supra-gingival oral irrigation plus ROH for 14 days. Group A patients were crossed-over to group C for 14 days (=day 28) after a professional prophylaxis. Group assignment was randomized by a coin toss, with the exception of group A subjects, who were self-selected as per recommendations of the internal review board for human subjects. GCF was sampled from 3 study teeth per patient and analyzed for IL-1β, PGE2, IL-10 and IFNγ by ELISA on days 0, 7, 14 and 28. Probing pocket depths (PPD), clinical attachment levels (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were measured by a calibrated examiner (TWS) on days 0, 14 and 28. Analysis of covariance was performed using SAS 6.12 and Proc Mixed with group and IL-1GT as the factors and the baseline levels as the covariate, with output being least squares means and least significant difference (LSD). Significant differences were declared if the p-value for the F-statistic was ≤0.05. Results: Oral irrigation plus ROH resulted in a significant reduction in PPD, BOP, GI and PI, as well as IL-β levels by 7 days and PGE2 levels by 14 days, relative to ROH or no oral hygiene. Interestingly, decreased IL-1β levels in patients using oral irrigation plus ROH was accompanied by a trend for increased levels of the "anti-inflammatory" cytokine IL-10. ROH reduced GI, BOP and PI, and PGE2 levels by 14 days, but had no effect on IL-1β or IL-10 levels relative to no oral hygiene. The effects of no oral hygiene were reversed by a prophy followed by oral irrigation plus ROH for 14 days. No clinical differences were evident between IL-1 GT (+) patients (n=11) and GT (-) patients (n=40), but the former had significantly elevated levels of GCF IL-10 and borderline increases in IL-1β (p=0.07). Conclusions: Oral irrigation with water for 14 days had an improved therapeutic benefit for AP over that of routine oral hygiene alone and this improvement was accompanied by a down-modulation of the pro-inflammatory cytokine profile in GCF.

Original languageEnglish (US)
Pages (from-to)134-143
Number of pages10
JournalJournal of Clinical Periodontology
Volume27
Issue number2
DOIs
StatePublished - Feb 2000

Fingerprint

Periodontitis
Oral Hygiene
Interleukin-1
Cytokines
Periodontal Index
Interleukin-10
Dinoprostone
Chronic Periodontitis
Water
Hemorrhage
Numismatics
Least-Squares Analysis
Interferons
Tooth
Anti-Inflammatory Agents
Enzyme-Linked Immunosorbent Assay
Genotype

Keywords

  • Cytokines
  • Gingival crevicular fluid
  • Inflammation
  • Interleukin-1β
  • Oral irrigation
  • Periodontitis

ASJC Scopus subject areas

  • Periodontics

Cite this

Clinical benefits of oral irrigation for periodontitis are related to reduction of pro-inflammatory cytokine levels and plaque. / Cutler, Christopher W.; Stanford, Thomas W.; Abraham, Celeste; Cederberg, Robert A.; Boardman, Thomas J.; Ross, Candy.

In: Journal of Clinical Periodontology, Vol. 27, No. 2, 02.2000, p. 134-143.

Research output: Contribution to journalArticle

Cutler, Christopher W. ; Stanford, Thomas W. ; Abraham, Celeste ; Cederberg, Robert A. ; Boardman, Thomas J. ; Ross, Candy. / Clinical benefits of oral irrigation for periodontitis are related to reduction of pro-inflammatory cytokine levels and plaque. In: Journal of Clinical Periodontology. 2000 ; Vol. 27, No. 2. pp. 134-143.
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title = "Clinical benefits of oral irrigation for periodontitis are related to reduction of pro-inflammatory cytokine levels and plaque",
abstract = "Background: Although a growing body of evidence indicates that oral irrigation with water has therapeutic benefits in periodontitis, the mechanisms of action have not been elucidated. The aims of this study were: (1) to analyze the effects of oral irrigation (Water Pik{\circledR} Oral Irrigator) on the clinical signs of adult periodontitis (AP) and on the levels of interleukin-1β (IL-β), prostaglandin-E2 (PGE2), interleukin-10 (IL-10) and interferon-γ (IFN-γ) in GCF, and (2) to analyze the influence of the periodontitis-related IL-1 genotype (IL-1GT) on these variables. Method: A single-center, blinded study in otherwise healthy humans (n=52) with localized mild to moderate AP was carried out, using the following groups: group A (n=12), no oral hygiene for 14 days; group B (n=20), routine oral hygiene (ROH) for 14 days; group C (n=20), supra-gingival oral irrigation plus ROH for 14 days. Group A patients were crossed-over to group C for 14 days (=day 28) after a professional prophylaxis. Group assignment was randomized by a coin toss, with the exception of group A subjects, who were self-selected as per recommendations of the internal review board for human subjects. GCF was sampled from 3 study teeth per patient and analyzed for IL-1β, PGE2, IL-10 and IFNγ by ELISA on days 0, 7, 14 and 28. Probing pocket depths (PPD), clinical attachment levels (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were measured by a calibrated examiner (TWS) on days 0, 14 and 28. Analysis of covariance was performed using SAS 6.12 and Proc Mixed with group and IL-1GT as the factors and the baseline levels as the covariate, with output being least squares means and least significant difference (LSD). Significant differences were declared if the p-value for the F-statistic was ≤0.05. Results: Oral irrigation plus ROH resulted in a significant reduction in PPD, BOP, GI and PI, as well as IL-β levels by 7 days and PGE2 levels by 14 days, relative to ROH or no oral hygiene. Interestingly, decreased IL-1β levels in patients using oral irrigation plus ROH was accompanied by a trend for increased levels of the {"}anti-inflammatory{"} cytokine IL-10. ROH reduced GI, BOP and PI, and PGE2 levels by 14 days, but had no effect on IL-1β or IL-10 levels relative to no oral hygiene. The effects of no oral hygiene were reversed by a prophy followed by oral irrigation plus ROH for 14 days. No clinical differences were evident between IL-1 GT (+) patients (n=11) and GT (-) patients (n=40), but the former had significantly elevated levels of GCF IL-10 and borderline increases in IL-1β (p=0.07). Conclusions: Oral irrigation with water for 14 days had an improved therapeutic benefit for AP over that of routine oral hygiene alone and this improvement was accompanied by a down-modulation of the pro-inflammatory cytokine profile in GCF.",
keywords = "Cytokines, Gingival crevicular fluid, Inflammation, Interleukin-1β, Oral irrigation, Periodontitis",
author = "Cutler, {Christopher W.} and Stanford, {Thomas W.} and Celeste Abraham and Cederberg, {Robert A.} and Boardman, {Thomas J.} and Candy Ross",
year = "2000",
month = "2",
doi = "10.1034/j.1600-051x.2000.027002134.x",
language = "English (US)",
volume = "27",
pages = "134--143",
journal = "Journal of Clinical Periodontology",
issn = "0303-6979",
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}

TY - JOUR

T1 - Clinical benefits of oral irrigation for periodontitis are related to reduction of pro-inflammatory cytokine levels and plaque

AU - Cutler, Christopher W.

AU - Stanford, Thomas W.

AU - Abraham, Celeste

AU - Cederberg, Robert A.

AU - Boardman, Thomas J.

AU - Ross, Candy

PY - 2000/2

Y1 - 2000/2

N2 - Background: Although a growing body of evidence indicates that oral irrigation with water has therapeutic benefits in periodontitis, the mechanisms of action have not been elucidated. The aims of this study were: (1) to analyze the effects of oral irrigation (Water Pik® Oral Irrigator) on the clinical signs of adult periodontitis (AP) and on the levels of interleukin-1β (IL-β), prostaglandin-E2 (PGE2), interleukin-10 (IL-10) and interferon-γ (IFN-γ) in GCF, and (2) to analyze the influence of the periodontitis-related IL-1 genotype (IL-1GT) on these variables. Method: A single-center, blinded study in otherwise healthy humans (n=52) with localized mild to moderate AP was carried out, using the following groups: group A (n=12), no oral hygiene for 14 days; group B (n=20), routine oral hygiene (ROH) for 14 days; group C (n=20), supra-gingival oral irrigation plus ROH for 14 days. Group A patients were crossed-over to group C for 14 days (=day 28) after a professional prophylaxis. Group assignment was randomized by a coin toss, with the exception of group A subjects, who were self-selected as per recommendations of the internal review board for human subjects. GCF was sampled from 3 study teeth per patient and analyzed for IL-1β, PGE2, IL-10 and IFNγ by ELISA on days 0, 7, 14 and 28. Probing pocket depths (PPD), clinical attachment levels (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were measured by a calibrated examiner (TWS) on days 0, 14 and 28. Analysis of covariance was performed using SAS 6.12 and Proc Mixed with group and IL-1GT as the factors and the baseline levels as the covariate, with output being least squares means and least significant difference (LSD). Significant differences were declared if the p-value for the F-statistic was ≤0.05. Results: Oral irrigation plus ROH resulted in a significant reduction in PPD, BOP, GI and PI, as well as IL-β levels by 7 days and PGE2 levels by 14 days, relative to ROH or no oral hygiene. Interestingly, decreased IL-1β levels in patients using oral irrigation plus ROH was accompanied by a trend for increased levels of the "anti-inflammatory" cytokine IL-10. ROH reduced GI, BOP and PI, and PGE2 levels by 14 days, but had no effect on IL-1β or IL-10 levels relative to no oral hygiene. The effects of no oral hygiene were reversed by a prophy followed by oral irrigation plus ROH for 14 days. No clinical differences were evident between IL-1 GT (+) patients (n=11) and GT (-) patients (n=40), but the former had significantly elevated levels of GCF IL-10 and borderline increases in IL-1β (p=0.07). Conclusions: Oral irrigation with water for 14 days had an improved therapeutic benefit for AP over that of routine oral hygiene alone and this improvement was accompanied by a down-modulation of the pro-inflammatory cytokine profile in GCF.

AB - Background: Although a growing body of evidence indicates that oral irrigation with water has therapeutic benefits in periodontitis, the mechanisms of action have not been elucidated. The aims of this study were: (1) to analyze the effects of oral irrigation (Water Pik® Oral Irrigator) on the clinical signs of adult periodontitis (AP) and on the levels of interleukin-1β (IL-β), prostaglandin-E2 (PGE2), interleukin-10 (IL-10) and interferon-γ (IFN-γ) in GCF, and (2) to analyze the influence of the periodontitis-related IL-1 genotype (IL-1GT) on these variables. Method: A single-center, blinded study in otherwise healthy humans (n=52) with localized mild to moderate AP was carried out, using the following groups: group A (n=12), no oral hygiene for 14 days; group B (n=20), routine oral hygiene (ROH) for 14 days; group C (n=20), supra-gingival oral irrigation plus ROH for 14 days. Group A patients were crossed-over to group C for 14 days (=day 28) after a professional prophylaxis. Group assignment was randomized by a coin toss, with the exception of group A subjects, who were self-selected as per recommendations of the internal review board for human subjects. GCF was sampled from 3 study teeth per patient and analyzed for IL-1β, PGE2, IL-10 and IFNγ by ELISA on days 0, 7, 14 and 28. Probing pocket depths (PPD), clinical attachment levels (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were measured by a calibrated examiner (TWS) on days 0, 14 and 28. Analysis of covariance was performed using SAS 6.12 and Proc Mixed with group and IL-1GT as the factors and the baseline levels as the covariate, with output being least squares means and least significant difference (LSD). Significant differences were declared if the p-value for the F-statistic was ≤0.05. Results: Oral irrigation plus ROH resulted in a significant reduction in PPD, BOP, GI and PI, as well as IL-β levels by 7 days and PGE2 levels by 14 days, relative to ROH or no oral hygiene. Interestingly, decreased IL-1β levels in patients using oral irrigation plus ROH was accompanied by a trend for increased levels of the "anti-inflammatory" cytokine IL-10. ROH reduced GI, BOP and PI, and PGE2 levels by 14 days, but had no effect on IL-1β or IL-10 levels relative to no oral hygiene. The effects of no oral hygiene were reversed by a prophy followed by oral irrigation plus ROH for 14 days. No clinical differences were evident between IL-1 GT (+) patients (n=11) and GT (-) patients (n=40), but the former had significantly elevated levels of GCF IL-10 and borderline increases in IL-1β (p=0.07). Conclusions: Oral irrigation with water for 14 days had an improved therapeutic benefit for AP over that of routine oral hygiene alone and this improvement was accompanied by a down-modulation of the pro-inflammatory cytokine profile in GCF.

KW - Cytokines

KW - Gingival crevicular fluid

KW - Inflammation

KW - Interleukin-1β

KW - Oral irrigation

KW - Periodontitis

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