Clinical characterization of in vivo inflammatory bowel disease with Raman spectroscopy

Isaac J. Pence, Dawn B. Beaulieu, Sara N. Horst, Xiaohong Bi, Alan J. Herline, David A. Schwartz, Anita Mahadevan-Jansen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn’s disease (CD), affects over 1 million Americans and 2 million Europeans, and the incidence is increasing worldwide. While these diseases require unique medical care, the differentiation between UC and CD lacks a gold standard, and therefore relies on long term follow up, success or failure of existing treatment, and recurrence of the disease. Here, we present colonoscopy-coupled fiber optic probe-based Raman spectroscopy as a minimally-invasive diagnostic tool for IBD of the colon (UC and Crohn’s colitis). This pilot in vivo study of subjects with existing IBD diagnoses of UC (n = 8), CD (n = 15), and normal control (n = 8) aimed to characterize spectral signatures of UC and CD. Samples were correlated with tissue pathology markers and endoscopic evaluation. The collected spectra were processed and analyzed using multivariate statistical techniques to identify spectral markers and discriminate IBD and disease classes. Confounding factors including the presence of active inflammation and the particular colon segment measured were investigated and integrated into the devised prediction algorithm, reaching 90% sensitivity and 75% specificity to CD from this in vivo data set. These results represent significant progress towards improved real-time classification for accurate and automated in vivo detection and discrimination of IBD during colonoscopy procedures.

Original languageEnglish (US)
Article number#276220
Pages (from-to)524-535
Number of pages12
JournalBiomedical Optics Express
Volume8
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Raman Spectrum Analysis
Ulcerative Colitis
Inflammatory Bowel Diseases
Crohn Disease
Raman spectroscopy
Colonoscopy
Colon
Colitis
Treatment Failure
markers
Pathology
Inflammation
Recurrence
Sensitivity and Specificity
Incidence
spectral signatures
pathology
discrimination
fiber optics
incidence

Keywords

  • Medical optics instrumentation
  • Raman spectroscopy
  • Spectroscopy, tissue diagnostics
  • Tissue characterization

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Pence, I. J., Beaulieu, D. B., Horst, S. N., Bi, X., Herline, A. J., Schwartz, D. A., & Mahadevan-Jansen, A. (2017). Clinical characterization of in vivo inflammatory bowel disease with Raman spectroscopy. Biomedical Optics Express, 8(2), 524-535. [#276220]. https://doi.org/10.1364/BOE.8.000524

Clinical characterization of in vivo inflammatory bowel disease with Raman spectroscopy. / Pence, Isaac J.; Beaulieu, Dawn B.; Horst, Sara N.; Bi, Xiaohong; Herline, Alan J.; Schwartz, David A.; Mahadevan-Jansen, Anita.

In: Biomedical Optics Express, Vol. 8, No. 2, #276220, 01.02.2017, p. 524-535.

Research output: Contribution to journalArticle

Pence, IJ, Beaulieu, DB, Horst, SN, Bi, X, Herline, AJ, Schwartz, DA & Mahadevan-Jansen, A 2017, 'Clinical characterization of in vivo inflammatory bowel disease with Raman spectroscopy', Biomedical Optics Express, vol. 8, no. 2, #276220, pp. 524-535. https://doi.org/10.1364/BOE.8.000524
Pence IJ, Beaulieu DB, Horst SN, Bi X, Herline AJ, Schwartz DA et al. Clinical characterization of in vivo inflammatory bowel disease with Raman spectroscopy. Biomedical Optics Express. 2017 Feb 1;8(2):524-535. #276220. https://doi.org/10.1364/BOE.8.000524
Pence, Isaac J. ; Beaulieu, Dawn B. ; Horst, Sara N. ; Bi, Xiaohong ; Herline, Alan J. ; Schwartz, David A. ; Mahadevan-Jansen, Anita. / Clinical characterization of in vivo inflammatory bowel disease with Raman spectroscopy. In: Biomedical Optics Express. 2017 ; Vol. 8, No. 2. pp. 524-535.
@article{92231344d7f04cdfa8e20ed274254b70,
title = "Clinical characterization of in vivo inflammatory bowel disease with Raman spectroscopy",
abstract = "Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn’s disease (CD), affects over 1 million Americans and 2 million Europeans, and the incidence is increasing worldwide. While these diseases require unique medical care, the differentiation between UC and CD lacks a gold standard, and therefore relies on long term follow up, success or failure of existing treatment, and recurrence of the disease. Here, we present colonoscopy-coupled fiber optic probe-based Raman spectroscopy as a minimally-invasive diagnostic tool for IBD of the colon (UC and Crohn’s colitis). This pilot in vivo study of subjects with existing IBD diagnoses of UC (n = 8), CD (n = 15), and normal control (n = 8) aimed to characterize spectral signatures of UC and CD. Samples were correlated with tissue pathology markers and endoscopic evaluation. The collected spectra were processed and analyzed using multivariate statistical techniques to identify spectral markers and discriminate IBD and disease classes. Confounding factors including the presence of active inflammation and the particular colon segment measured were investigated and integrated into the devised prediction algorithm, reaching 90{\%} sensitivity and 75{\%} specificity to CD from this in vivo data set. These results represent significant progress towards improved real-time classification for accurate and automated in vivo detection and discrimination of IBD during colonoscopy procedures.",
keywords = "Medical optics instrumentation, Raman spectroscopy, Spectroscopy, tissue diagnostics, Tissue characterization",
author = "Pence, {Isaac J.} and Beaulieu, {Dawn B.} and Horst, {Sara N.} and Xiaohong Bi and Herline, {Alan J.} and Schwartz, {David A.} and Anita Mahadevan-Jansen",
year = "2017",
month = "2",
day = "1",
doi = "10.1364/BOE.8.000524",
language = "English (US)",
volume = "8",
pages = "524--535",
journal = "Biomedical Optics Express",
issn = "2156-7085",
publisher = "The Optical Society",
number = "2",

}

TY - JOUR

T1 - Clinical characterization of in vivo inflammatory bowel disease with Raman spectroscopy

AU - Pence, Isaac J.

AU - Beaulieu, Dawn B.

AU - Horst, Sara N.

AU - Bi, Xiaohong

AU - Herline, Alan J.

AU - Schwartz, David A.

AU - Mahadevan-Jansen, Anita

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn’s disease (CD), affects over 1 million Americans and 2 million Europeans, and the incidence is increasing worldwide. While these diseases require unique medical care, the differentiation between UC and CD lacks a gold standard, and therefore relies on long term follow up, success or failure of existing treatment, and recurrence of the disease. Here, we present colonoscopy-coupled fiber optic probe-based Raman spectroscopy as a minimally-invasive diagnostic tool for IBD of the colon (UC and Crohn’s colitis). This pilot in vivo study of subjects with existing IBD diagnoses of UC (n = 8), CD (n = 15), and normal control (n = 8) aimed to characterize spectral signatures of UC and CD. Samples were correlated with tissue pathology markers and endoscopic evaluation. The collected spectra were processed and analyzed using multivariate statistical techniques to identify spectral markers and discriminate IBD and disease classes. Confounding factors including the presence of active inflammation and the particular colon segment measured were investigated and integrated into the devised prediction algorithm, reaching 90% sensitivity and 75% specificity to CD from this in vivo data set. These results represent significant progress towards improved real-time classification for accurate and automated in vivo detection and discrimination of IBD during colonoscopy procedures.

AB - Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn’s disease (CD), affects over 1 million Americans and 2 million Europeans, and the incidence is increasing worldwide. While these diseases require unique medical care, the differentiation between UC and CD lacks a gold standard, and therefore relies on long term follow up, success or failure of existing treatment, and recurrence of the disease. Here, we present colonoscopy-coupled fiber optic probe-based Raman spectroscopy as a minimally-invasive diagnostic tool for IBD of the colon (UC and Crohn’s colitis). This pilot in vivo study of subjects with existing IBD diagnoses of UC (n = 8), CD (n = 15), and normal control (n = 8) aimed to characterize spectral signatures of UC and CD. Samples were correlated with tissue pathology markers and endoscopic evaluation. The collected spectra were processed and analyzed using multivariate statistical techniques to identify spectral markers and discriminate IBD and disease classes. Confounding factors including the presence of active inflammation and the particular colon segment measured were investigated and integrated into the devised prediction algorithm, reaching 90% sensitivity and 75% specificity to CD from this in vivo data set. These results represent significant progress towards improved real-time classification for accurate and automated in vivo detection and discrimination of IBD during colonoscopy procedures.

KW - Medical optics instrumentation

KW - Raman spectroscopy

KW - Spectroscopy, tissue diagnostics

KW - Tissue characterization

UR - http://www.scopus.com/inward/record.url?scp=85011564541&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85011564541&partnerID=8YFLogxK

U2 - 10.1364/BOE.8.000524

DO - 10.1364/BOE.8.000524

M3 - Article

AN - SCOPUS:85011564541

VL - 8

SP - 524

EP - 535

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 2

M1 - #276220

ER -