Cloning and characterization of glial cell line-derived neurotrophic factor receptor-B: A novel receptor for members of glial cell line-derived neurotrophic factor family of neurotrophic factors

C. Y. Wang, J. Ni, H. Jiang, T. A. Hsu, M. Dugich-Djordjevic, L. Feng, M. Zhang, Lin Mei, R. Gentz, B. Lu

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Glial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor with diverse biological functions. Signal transduction of GDNF is mediated by binding to a glycosylphosphatidylinositol (GPI)-linked receptor GDNFR-α and activation of c-RET tyrosine kinase. The recent discovery of a new GDNF homolog neurturin raises the possibility that multiple receptors exist for the members in the GDNF family. Here we report isolation of the gene encoding a new receptor called GDNFR-β. Sequence analysis indicated that GDNFR-β is also a GPI-linked protein, with 47% identity to GDNFR-α. The GDNFR-β transcript was preferentially expressed in the brain, spleen and lung, but moderate levels of GDNFR-β mRNA were also found in kidney and the entire gastrointestinal track. In situ hybridization revealed high expression levels in the entorhinal cortex and olfactory bulb, followed by cortex, septum, inferior and superior colliculus, and zona inserta. A laminar pattern of expression was detected in layer III of the cortex. Treatment with GDNF of PC12 cells transfected with the GDNFR-β gene activated mitogeo-activated protein kinase (MAPK) and elicited neurite outgrowth. GDNFR-α and GDNFR-β together form a new family of GPI-linked receptors for GDNF-like molecules.

Original languageEnglish (US)
Pages (from-to)7-14
Number of pages8
JournalNeuroscience
Volume83
Issue number1
DOIs
StatePublished - Mar 1 1998

Fingerprint

Glial Cell Line-Derived Neurotrophic Factor Receptors
Glial Cell Line-Derived Neurotrophic Factor
Nerve Growth Factors
Organism Cloning
Glycosylphosphatidylinositols
GPI-Linked Proteins
Neurturin
Inferior Colliculi
Entorhinal Cortex
Superior Colliculi
Olfactory Bulb
PC12 Cells
Herpes Zoster
Protein-Tyrosine Kinases
Protein Kinases
Genes
In Situ Hybridization
Sequence Analysis
Signal Transduction
Spleen

Keywords

  • C-RET
  • GPI-linked receptors
  • MAP kinase
  • Neurturin
  • PC12 cells

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cloning and characterization of glial cell line-derived neurotrophic factor receptor-B : A novel receptor for members of glial cell line-derived neurotrophic factor family of neurotrophic factors. / Wang, C. Y.; Ni, J.; Jiang, H.; Hsu, T. A.; Dugich-Djordjevic, M.; Feng, L.; Zhang, M.; Mei, Lin; Gentz, R.; Lu, B.

In: Neuroscience, Vol. 83, No. 1, 01.03.1998, p. 7-14.

Research output: Contribution to journalArticle

Wang, C. Y. ; Ni, J. ; Jiang, H. ; Hsu, T. A. ; Dugich-Djordjevic, M. ; Feng, L. ; Zhang, M. ; Mei, Lin ; Gentz, R. ; Lu, B. / Cloning and characterization of glial cell line-derived neurotrophic factor receptor-B : A novel receptor for members of glial cell line-derived neurotrophic factor family of neurotrophic factors. In: Neuroscience. 1998 ; Vol. 83, No. 1. pp. 7-14.
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