Cloning and expression of the rat nephrin homolog

Heikki Ahola, Shixuan Wang, Pauliina Luimula, Marja Liisa Solin, Lawrence B. Holzman, Harry Holthöfer

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Despite of the increased availability of genetically modified mouse strains, the experimental models in the rat have provided the most widely employed and versatile models for the study of renal pathophysiology and functional genetics. The identification of the human gene mutated in the congenital nephrotic syndrome of the Finnish type (NPHS1) has recently been reported, and its protein product has been termed nephrin. Here we report the molecular cloning and characterization of rat nephrin cDNA. Rat nephrin cDNA has an open reading frame of 3705 bp, shows 82% sequence identity with human nephrin cDNA, and shows characteristic rat-specific splicing variants. The translated nucleotide sequence has 89% sequence identity at the amino acid level. The signal sequence, glycosylation, and cysteine localization patterns are nearly identical to those of human nephrin. As in the human, the rat nephrin transcript is expressed in a tissue-restricted pattern. Antipeptide antibodies raised to the intracellular nephrin-specific domain identified immunoreactivity exclusively within the rat kidney glomerulus by indirect immunofluorescence. Initial results with semiquantitative reverse transcriptase-polymerase chain reaction analysis showed a remarkable down- regulation of nephrin-specific mRNA in the puromycin nephrosis of the rat.

Original languageEnglish (US)
Pages (from-to)907-913
Number of pages7
JournalAmerican Journal of Pathology
Volume155
Issue number3
DOIs
StatePublished - Jan 1 1999

Fingerprint

Organism Cloning
Complementary DNA
Kidney Glomerulus
Nephrosis
Puromycin
Forensic Anthropology
nephrin
Molecular Cloning
Indirect Fluorescent Antibody Technique
Protein Sorting Signals
Reverse Transcriptase Polymerase Chain Reaction
Glycosylation
Open Reading Frames
Cysteine
Theoretical Models
Down-Regulation
Kidney
Amino Acids
Messenger RNA
Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Ahola, H., Wang, S., Luimula, P., Solin, M. L., Holzman, L. B., & Holthöfer, H. (1999). Cloning and expression of the rat nephrin homolog. American Journal of Pathology, 155(3), 907-913. https://doi.org/10.1016/S0002-9440(10)65190-5

Cloning and expression of the rat nephrin homolog. / Ahola, Heikki; Wang, Shixuan; Luimula, Pauliina; Solin, Marja Liisa; Holzman, Lawrence B.; Holthöfer, Harry.

In: American Journal of Pathology, Vol. 155, No. 3, 01.01.1999, p. 907-913.

Research output: Contribution to journalArticle

Ahola, H, Wang, S, Luimula, P, Solin, ML, Holzman, LB & Holthöfer, H 1999, 'Cloning and expression of the rat nephrin homolog', American Journal of Pathology, vol. 155, no. 3, pp. 907-913. https://doi.org/10.1016/S0002-9440(10)65190-5
Ahola, Heikki ; Wang, Shixuan ; Luimula, Pauliina ; Solin, Marja Liisa ; Holzman, Lawrence B. ; Holthöfer, Harry. / Cloning and expression of the rat nephrin homolog. In: American Journal of Pathology. 1999 ; Vol. 155, No. 3. pp. 907-913.
@article{1275939570f8407a98597cea62528e91,
title = "Cloning and expression of the rat nephrin homolog",
abstract = "Despite of the increased availability of genetically modified mouse strains, the experimental models in the rat have provided the most widely employed and versatile models for the study of renal pathophysiology and functional genetics. The identification of the human gene mutated in the congenital nephrotic syndrome of the Finnish type (NPHS1) has recently been reported, and its protein product has been termed nephrin. Here we report the molecular cloning and characterization of rat nephrin cDNA. Rat nephrin cDNA has an open reading frame of 3705 bp, shows 82{\%} sequence identity with human nephrin cDNA, and shows characteristic rat-specific splicing variants. The translated nucleotide sequence has 89{\%} sequence identity at the amino acid level. The signal sequence, glycosylation, and cysteine localization patterns are nearly identical to those of human nephrin. As in the human, the rat nephrin transcript is expressed in a tissue-restricted pattern. Antipeptide antibodies raised to the intracellular nephrin-specific domain identified immunoreactivity exclusively within the rat kidney glomerulus by indirect immunofluorescence. Initial results with semiquantitative reverse transcriptase-polymerase chain reaction analysis showed a remarkable down- regulation of nephrin-specific mRNA in the puromycin nephrosis of the rat.",
author = "Heikki Ahola and Shixuan Wang and Pauliina Luimula and Solin, {Marja Liisa} and Holzman, {Lawrence B.} and Harry Holth{\"o}fer",
year = "1999",
month = "1",
day = "1",
doi = "10.1016/S0002-9440(10)65190-5",
language = "English (US)",
volume = "155",
pages = "907--913",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Cloning and expression of the rat nephrin homolog

AU - Ahola, Heikki

AU - Wang, Shixuan

AU - Luimula, Pauliina

AU - Solin, Marja Liisa

AU - Holzman, Lawrence B.

AU - Holthöfer, Harry

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Despite of the increased availability of genetically modified mouse strains, the experimental models in the rat have provided the most widely employed and versatile models for the study of renal pathophysiology and functional genetics. The identification of the human gene mutated in the congenital nephrotic syndrome of the Finnish type (NPHS1) has recently been reported, and its protein product has been termed nephrin. Here we report the molecular cloning and characterization of rat nephrin cDNA. Rat nephrin cDNA has an open reading frame of 3705 bp, shows 82% sequence identity with human nephrin cDNA, and shows characteristic rat-specific splicing variants. The translated nucleotide sequence has 89% sequence identity at the amino acid level. The signal sequence, glycosylation, and cysteine localization patterns are nearly identical to those of human nephrin. As in the human, the rat nephrin transcript is expressed in a tissue-restricted pattern. Antipeptide antibodies raised to the intracellular nephrin-specific domain identified immunoreactivity exclusively within the rat kidney glomerulus by indirect immunofluorescence. Initial results with semiquantitative reverse transcriptase-polymerase chain reaction analysis showed a remarkable down- regulation of nephrin-specific mRNA in the puromycin nephrosis of the rat.

AB - Despite of the increased availability of genetically modified mouse strains, the experimental models in the rat have provided the most widely employed and versatile models for the study of renal pathophysiology and functional genetics. The identification of the human gene mutated in the congenital nephrotic syndrome of the Finnish type (NPHS1) has recently been reported, and its protein product has been termed nephrin. Here we report the molecular cloning and characterization of rat nephrin cDNA. Rat nephrin cDNA has an open reading frame of 3705 bp, shows 82% sequence identity with human nephrin cDNA, and shows characteristic rat-specific splicing variants. The translated nucleotide sequence has 89% sequence identity at the amino acid level. The signal sequence, glycosylation, and cysteine localization patterns are nearly identical to those of human nephrin. As in the human, the rat nephrin transcript is expressed in a tissue-restricted pattern. Antipeptide antibodies raised to the intracellular nephrin-specific domain identified immunoreactivity exclusively within the rat kidney glomerulus by indirect immunofluorescence. Initial results with semiquantitative reverse transcriptase-polymerase chain reaction analysis showed a remarkable down- regulation of nephrin-specific mRNA in the puromycin nephrosis of the rat.

UR - http://www.scopus.com/inward/record.url?scp=0032886883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032886883&partnerID=8YFLogxK

U2 - 10.1016/S0002-9440(10)65190-5

DO - 10.1016/S0002-9440(10)65190-5

M3 - Article

VL - 155

SP - 907

EP - 913

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 3

ER -