Cloning and functional expression of the human type 1 sigma receptor (hSigmaR1)

Ramesh Kekuda, Puttur D Prasad, You Jun Fei, Frederick H. Leibach, Vadivel Ganapathy

Research output: Contribution to journalArticle

348 Citations (Scopus)

Abstract

We have isolated a cDNA clone from a human placental choriocarcinoma cell (JAR) cDNA library which codes for a functional type 1 sigma receptor. An RT-PCR product obtained from guinea pig kidney mRNA using primers specific for the guinea pig sigma receptor cDNA was used to screen the cDNA library. The hSigmaR1 cDNA predicts a protein of 223 amino acids with a single putative transmembrane domain. The amino acid sequence exhibits 93% identity with the guinea pig sigma receptor. When functionally expressed in HeLa cells, the hSigmaR1 cDNA enhances the binding of [3H]-haloperidol, a sigma receptor ligand, to the HeLa cell membranes. The inhibitor specificity of the cloned hSigmaR1 indicates that it is the type 1 sigma receptor. Several human tissues, including placenta, liver, and brain, and several human cell lines express the SigmaR1 mRNA (1.7 kb) to a variable extent.

Original languageEnglish (US)
Pages (from-to)553-558
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume229
Issue number2
DOIs
StatePublished - Dec 13 1996

Fingerprint

Cloning
sigma Receptors
Organism Cloning
Complementary DNA
Guinea Pigs
Gene Library
HeLa Cells
Amino Acids
Messenger RNA
Choriocarcinoma
Haloperidol
Cell membranes
Liver
Placenta
Amino Acid Sequence
Brain
Clone Cells
Cells
Cell Membrane
Tissue

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cloning and functional expression of the human type 1 sigma receptor (hSigmaR1). / Kekuda, Ramesh; Prasad, Puttur D; Fei, You Jun; Leibach, Frederick H.; Ganapathy, Vadivel.

In: Biochemical and Biophysical Research Communications, Vol. 229, No. 2, 13.12.1996, p. 553-558.

Research output: Contribution to journalArticle

Kekuda, Ramesh ; Prasad, Puttur D ; Fei, You Jun ; Leibach, Frederick H. ; Ganapathy, Vadivel. / Cloning and functional expression of the human type 1 sigma receptor (hSigmaR1). In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 229, No. 2. pp. 553-558.
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