Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal

Zhi Nong Wang, Hiroshi Takemori, Sunil Krishna Halder, Yasuki Nonaka, Mitsuhiro Okamoto

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA done, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/threonine kinases in the SNF1/AMPK family was isolated. An in vitro kinase assay demonstrated that SIK protein had autophosphorylation activity. Northern blot revealed that SIK mRNA levels were markedly augmented by ACTH treatment both in rat adrenal glands and in Y1 cells. SIK may play an important role in the regulation of adrenocortical functions in response to high plasma salt and ACTH stimulation.

Original languageEnglish (US)
Pages (from-to)135-139
Number of pages5
JournalFEBS Letters
Volume453
Issue number1-2
DOIs
StatePublished - Jun 18 1999

Fingerprint

AMP-Activated Protein Kinases
Cloning
Nutrition
Rats
Organism Cloning
Phosphotransferases
Salts
Diet
Adrenocorticotropic Hormone
Complementary DNA
Protein-Serine-Threonine Kinases
Adrenal Glands
Northern Blotting
Protein Kinases
AMP-activated protein kinase kinase
Assays
Genes
Tissue
Plasmas
Amino Acids

Keywords

  • Adrenocortical function
  • Adrenocorticotropic hormone
  • Protein serine/threonine kinase
  • SNF1/AMPK
  • Salt inducible kinase
  • Y1 mouse adrenocortical tumor cell

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal. / Wang, Zhi Nong; Takemori, Hiroshi; Halder, Sunil Krishna; Nonaka, Yasuki; Okamoto, Mitsuhiro.

In: FEBS Letters, Vol. 453, No. 1-2, 18.06.1999, p. 135-139.

Research output: Contribution to journalArticle

Wang, ZN, Takemori, H, Halder, SK, Nonaka, Y & Okamoto, M 1999, 'Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal', FEBS Letters, vol. 453, no. 1-2, pp. 135-139. https://doi.org/10.1016/S0014-5793(99)00708-5
Wang, Zhi Nong ; Takemori, Hiroshi ; Halder, Sunil Krishna ; Nonaka, Yasuki ; Okamoto, Mitsuhiro. / Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal. In: FEBS Letters. 1999 ; Vol. 453, No. 1-2. pp. 135-139.
@article{199b30b4e08b4578ac58309bc64f945b,
title = "Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal",
abstract = "PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA done, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/threonine kinases in the SNF1/AMPK family was isolated. An in vitro kinase assay demonstrated that SIK protein had autophosphorylation activity. Northern blot revealed that SIK mRNA levels were markedly augmented by ACTH treatment both in rat adrenal glands and in Y1 cells. SIK may play an important role in the regulation of adrenocortical functions in response to high plasma salt and ACTH stimulation.",
keywords = "Adrenocortical function, Adrenocorticotropic hormone, Protein serine/threonine kinase, SNF1/AMPK, Salt inducible kinase, Y1 mouse adrenocortical tumor cell",
author = "Wang, {Zhi Nong} and Hiroshi Takemori and Halder, {Sunil Krishna} and Yasuki Nonaka and Mitsuhiro Okamoto",
year = "1999",
month = "6",
day = "18",
doi = "10.1016/S0014-5793(99)00708-5",
language = "English (US)",
volume = "453",
pages = "135--139",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal

AU - Wang, Zhi Nong

AU - Takemori, Hiroshi

AU - Halder, Sunil Krishna

AU - Nonaka, Yasuki

AU - Okamoto, Mitsuhiro

PY - 1999/6/18

Y1 - 1999/6/18

N2 - PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA done, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/threonine kinases in the SNF1/AMPK family was isolated. An in vitro kinase assay demonstrated that SIK protein had autophosphorylation activity. Northern blot revealed that SIK mRNA levels were markedly augmented by ACTH treatment both in rat adrenal glands and in Y1 cells. SIK may play an important role in the regulation of adrenocortical functions in response to high plasma salt and ACTH stimulation.

AB - PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA done, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/threonine kinases in the SNF1/AMPK family was isolated. An in vitro kinase assay demonstrated that SIK protein had autophosphorylation activity. Northern blot revealed that SIK mRNA levels were markedly augmented by ACTH treatment both in rat adrenal glands and in Y1 cells. SIK may play an important role in the regulation of adrenocortical functions in response to high plasma salt and ACTH stimulation.

KW - Adrenocortical function

KW - Adrenocorticotropic hormone

KW - Protein serine/threonine kinase

KW - SNF1/AMPK

KW - Salt inducible kinase

KW - Y1 mouse adrenocortical tumor cell

UR - http://www.scopus.com/inward/record.url?scp=0032791608&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032791608&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(99)00708-5

DO - 10.1016/S0014-5793(99)00708-5

M3 - Article

VL - 453

SP - 135

EP - 139

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-2

ER -