Abstract
PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA done, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/threonine kinases in the SNF1/AMPK family was isolated. An in vitro kinase assay demonstrated that SIK protein had autophosphorylation activity. Northern blot revealed that SIK mRNA levels were markedly augmented by ACTH treatment both in rat adrenal glands and in Y1 cells. SIK may play an important role in the regulation of adrenocortical functions in response to high plasma salt and ACTH stimulation.
Original language | English (US) |
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Pages (from-to) | 135-139 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 453 |
Issue number | 1-2 |
DOIs | |
State | Published - Jun 18 1999 |
Keywords
- Adrenocortical function
- Adrenocorticotropic hormone
- Protein serine/threonine kinase
- SNF1/AMPK
- Salt inducible kinase
- Y1 mouse adrenocortical tumor cell
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology