Cloning of the cDNA coding for rat brain CMP-NeuAc:GD3 α2-8 sialyltransferase

Guichao Zeng, Luoyi Gao, Robert K Yu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The cDNA coding for GT3-synthase has been cloned from a rat fetal brain cDNA library. The complete nucleotide sequence and the deduced amino acid sequence of the rat GT3-synthase cDNA were highly homologous to those of the mouse GT3-synthase. Quantitative RT-PCR showed that, as a key enzyme for the synthesis of 'c'-series gangliosides, the expression of GT3-synthase was developmentally regulated in embryonic rat brains.

Original languageEnglish (US)
Pages (from-to)131-134
Number of pages4
JournalGene
Volume187
Issue number1
DOIs
StatePublished - Mar 10 1997

Fingerprint

Sialyltransferases
Cytidine Monophosphate
Organism Cloning
Complementary DNA
Brain
Gene Library
Amino Acid Sequence
Polymerase Chain Reaction
Enzymes

Keywords

  • GT3-synthase
  • Gangliosides
  • Gene expression
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning of the cDNA coding for rat brain CMP-NeuAc:GD3 α2-8 sialyltransferase. / Zeng, Guichao; Gao, Luoyi; Yu, Robert K.

In: Gene, Vol. 187, No. 1, 10.03.1997, p. 131-134.

Research output: Contribution to journalArticle

Zeng, Guichao ; Gao, Luoyi ; Yu, Robert K. / Cloning of the cDNA coding for rat brain CMP-NeuAc:GD3 α2-8 sialyltransferase. In: Gene. 1997 ; Vol. 187, No. 1. pp. 131-134.
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