Cloning of the human Gfi-1 gene and its mapping to chromosome region 1p22

Terry Roberts, John Kenneth Cowell

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Recently the rat and mouse Growth Factor Independence (Gfi-1) genes have been cloned. This gene allows cells in culture to overcome the depletion of growth factors in the culture medium and maintain their proliferative potential. As part of a cloning strategy to isolated genes from human chromosome 1p22 which are associated with a constitutional chromosome translocation from a patient with stage 4S neuroblastoma, we have identified the human homologue of the Gfi gene and defined a 50 Kb map position within a well characterised YAC contig from the region. The full length cDNA sequence is 81% homologous with the rodent counterparts and, at the protein level, is even more highly conserved.

Original languageEnglish (US)
Pages (from-to)1003-1005
Number of pages3
JournalOncogene
Volume14
Issue number8
DOIs
StatePublished - Jan 1 1997

Fingerprint

Chromosome Mapping
Organism Cloning
Chromosomes
Genes
Intercellular Signaling Peptides and Proteins
Human Chromosomes
Neuroblastoma
Culture Media
Rodentia
Complementary DNA
Cell Culture Techniques
Proteins

Keywords

  • CDNA sequence
  • Chromosome 1p22
  • Human Gfi gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Cloning of the human Gfi-1 gene and its mapping to chromosome region 1p22. / Roberts, Terry; Cowell, John Kenneth.

In: Oncogene, Vol. 14, No. 8, 01.01.1997, p. 1003-1005.

Research output: Contribution to journalArticle

@article{6024e761896547c88536b660ff943ad9,
title = "Cloning of the human Gfi-1 gene and its mapping to chromosome region 1p22",
abstract = "Recently the rat and mouse Growth Factor Independence (Gfi-1) genes have been cloned. This gene allows cells in culture to overcome the depletion of growth factors in the culture medium and maintain their proliferative potential. As part of a cloning strategy to isolated genes from human chromosome 1p22 which are associated with a constitutional chromosome translocation from a patient with stage 4S neuroblastoma, we have identified the human homologue of the Gfi gene and defined a 50 Kb map position within a well characterised YAC contig from the region. The full length cDNA sequence is 81{\%} homologous with the rodent counterparts and, at the protein level, is even more highly conserved.",
keywords = "CDNA sequence, Chromosome 1p22, Human Gfi gene",
author = "Terry Roberts and Cowell, {John Kenneth}",
year = "1997",
month = "1",
day = "1",
doi = "10.1038/sj.onc.1200910",
language = "English (US)",
volume = "14",
pages = "1003--1005",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "8",

}

TY - JOUR

T1 - Cloning of the human Gfi-1 gene and its mapping to chromosome region 1p22

AU - Roberts, Terry

AU - Cowell, John Kenneth

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Recently the rat and mouse Growth Factor Independence (Gfi-1) genes have been cloned. This gene allows cells in culture to overcome the depletion of growth factors in the culture medium and maintain their proliferative potential. As part of a cloning strategy to isolated genes from human chromosome 1p22 which are associated with a constitutional chromosome translocation from a patient with stage 4S neuroblastoma, we have identified the human homologue of the Gfi gene and defined a 50 Kb map position within a well characterised YAC contig from the region. The full length cDNA sequence is 81% homologous with the rodent counterparts and, at the protein level, is even more highly conserved.

AB - Recently the rat and mouse Growth Factor Independence (Gfi-1) genes have been cloned. This gene allows cells in culture to overcome the depletion of growth factors in the culture medium and maintain their proliferative potential. As part of a cloning strategy to isolated genes from human chromosome 1p22 which are associated with a constitutional chromosome translocation from a patient with stage 4S neuroblastoma, we have identified the human homologue of the Gfi gene and defined a 50 Kb map position within a well characterised YAC contig from the region. The full length cDNA sequence is 81% homologous with the rodent counterparts and, at the protein level, is even more highly conserved.

KW - CDNA sequence

KW - Chromosome 1p22

KW - Human Gfi gene

UR - http://www.scopus.com/inward/record.url?scp=0030902113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030902113&partnerID=8YFLogxK

U2 - 10.1038/sj.onc.1200910

DO - 10.1038/sj.onc.1200910

M3 - Article

C2 - 9051000

AN - SCOPUS:0030902113

VL - 14

SP - 1003

EP - 1005

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 8

ER -