Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter B(o) from a human placental choriocarcinoma cell line

Ramesh Kekuda, Puttur D Prasad, You Jun Fei, Viviana Torres-Zamorano, Srish Sinha, Teresa L. Yang-Feng, Frederick H. Leibach, Vadivel Ganapathy

Research output: Contribution to journalArticle

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Abstract

We have isolated a cDNA from a human placental choriocarcinoma cell cDNA library which, when expressed in HeLa cells, induces a Na+-dependent amino acid transport system with preference for zwitterionic amino acids. Anionic amino acids, cationic amino acids, imino acids, and N-methylated amino acids are excluded by this system. These characteristics are identical to those described for the amino acid transporter B(o). When expressed in Xenopus laevis oocytes that do not have detectable endogenous activity of the amino acid transporter B(o), the cloned transporter increases alanine transport in the oocytes severalfold and induces alanine-evoked inward currents in the presence of Na+. The cDNA codes for a polypeptide containing 541 amino acids with 10 putative transmembrane domains. Amino acid sequence homology predicts this transporter (hATB(o)) to be a member of a superfamily consisting of the glutamate transporters, the neutral amino acid transport system ASCT, and the insulin-activable neutral/anionic amino acid transporter. Chromosomal assignment studies with somatic cell hybrid analysis and fluorescent in situ hybridization have located the ATB(o) gene to human chromosome 19q13.3.

Original languageEnglish (US)
Pages (from-to)18657-18661
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number31
DOIs
StatePublished - Oct 7 1996

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Neutral Amino Acid Transport Systems
Choriocarcinoma
Cloning
Organism Cloning
Amino Acid Transport Systems
Sodium
Cells
Amino Acids
Cell Line
Alanine
Acidic Amino Acid Transport Systems
Oocytes
Complementary DNA
Imino Acids
Amino Acid Transport System X-AG
Amino Acid Sequence Homology
Hybrid Cells
Xenopus laevis
Human Chromosomes
Fluorescence In Situ Hybridization

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter B(o) from a human placental choriocarcinoma cell line. / Kekuda, Ramesh; Prasad, Puttur D; Fei, You Jun; Torres-Zamorano, Viviana; Sinha, Srish; Yang-Feng, Teresa L.; Leibach, Frederick H.; Ganapathy, Vadivel.

In: Journal of Biological Chemistry, Vol. 271, No. 31, 07.10.1996, p. 18657-18661.

Research output: Contribution to journalArticle

Kekuda, Ramesh ; Prasad, Puttur D ; Fei, You Jun ; Torres-Zamorano, Viviana ; Sinha, Srish ; Yang-Feng, Teresa L. ; Leibach, Frederick H. ; Ganapathy, Vadivel. / Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter B(o) from a human placental choriocarcinoma cell line. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 31. pp. 18657-18661.
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