Co-distribution of cysteine cathepsins and matrix metalloproteases in human dentin

Polliana Mendes Candia Scaffa, Lorenzo Breschi, Annalisa Mazzoni, Cristina de Mattos Pimenta Vidal, Rosa Curci, Fabianni Apolonio, Pietro Gobbi, David Pashley, Leo Tjäderhane, Ivarne Luis dos Santos Tersariol, Fábio Dupart Nascimento, Marcela Rocha Carrilho

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

It has been hypothesized that cysteine cathepsins (CTs) along with matrix metalloproteases (MMPs) may work in conjunction in the proteolysis of mature dentin matrix. The aim of this study was to verify simultaneously the distribution and presence of cathepsins B (CT-B) and K (CT-K) in partially demineralized dentin; and further to evaluate the activity of CTs and MMPs in the same tissue. The distribution of CT-B and CT-K in sound human dentin was assessed by immunohistochemistry. A double-immunolabeling technique was used to identify, at once, the occurrence of those enzymes in dentin. Activities of CTs and MMPs in dentin extracts were evaluated spectrofluorometrically. In addition, in situ gelatinolytic activity of dentin was assayed by zymography. The results revealed the distribution of CT-B and CT-K along the dentin organic matrix and also indicated co-occurrence of MMPs and CTs in that tissue. The enzyme kinetics studies showed proteolytic activity in dentin extracts for both classes of proteases. Furthermore, it was observed that, at least for sound human dentin matrices, the activity of MMPs seems to be predominant over the CTs one.

Original languageEnglish (US)
Pages (from-to)101-107
Number of pages7
JournalArchives of Oral Biology
Volume74
DOIs
StatePublished - Feb 1 2017

Keywords

  • Cysteine cathepsins
  • Dentin
  • Immunohistochemistry
  • Matrix metalloproteases
  • Proteolytic activity

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

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    Scaffa, P. M. C., Breschi, L., Mazzoni, A., Vidal, C. D. M. P., Curci, R., Apolonio, F., Gobbi, P., Pashley, D., Tjäderhane, L., Tersariol, I. L. D. S., Nascimento, F. D., & Carrilho, M. R. (2017). Co-distribution of cysteine cathepsins and matrix metalloproteases in human dentin. Archives of Oral Biology, 74, 101-107. https://doi.org/10.1016/j.archoralbio.2016.11.011