Coexpression of CD71 and CD117 Identifies an Early Unipotent Neutrophil Progenitor Population in Human Bone Marrow

Huy Q. Dinh, Tobias Eggert, Melissa A. Meyer, Yanfang Peipei Zhu, Claire E. Olingy, Ryan Llewellyn, Runpei Wu, Catherine C. Hedrick

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Neutrophils are the most abundant peripheral immune cells and thus, are continually replenished by bone marrow-derived progenitors. Still, how newly identified neutrophil subsets fit into the bone marrow neutrophil lineage remains unclear. Here, we use mass cytometry to show that two recently defined human neutrophil progenitor populations contain a homogeneous progenitor subset we term “early neutrophil progenitors” (eNePs) (LinCD66b+CD117+CD71+). Surface marker- and RNA-expression analyses, together with in vitro colony formation and in vivo adoptive humanized mouse transfers, indicate that eNePs are the earliest human neutrophil progenitors. Furthermore, we identified CD71 as a marker associated with the earliest neutrophil developmental stages. Expression of CD71 marks proliferating neutrophils, which were expanded in the blood of melanoma patients and detectable in blood and tumors from lung cancer patients. In summary, we establish CD117+CD71+ eNeP as the inceptive human neutrophil progenitor and propose a refined model of the neutrophil developmental lineage in bone marrow.

Original languageEnglish (US)
Pages (from-to)319-334.e6
Issue number2
StatePublished - Aug 18 2020
Externally publishedYes


  • CD71
  • bone marrow
  • cancer
  • neutrophil development
  • neutrophil progenitors
  • neutrophils
  • promyelocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases


Dive into the research topics of 'Coexpression of CD71 and CD117 Identifies an Early Unipotent Neutrophil Progenitor Population in Human Bone Marrow'. Together they form a unique fingerprint.

Cite this