Cognitive enhancement with rosiglitazone links the hippocampal PPARγ and ERK MAPK signaling pathways

Larry A. Denner, Jennifer Rodriguez-Rivera, Sigmund J. Haidacher, Jordan B. Jahrling, J. Russ Carmical, Caterina M. Hernandez, Yingxin Zhao, Rovshan G. Sadygov, Jonathan M. Starkey, Heidi Spratt, Bruce A. Luxon, Thomas G. Wood, Kelly T. Dineley

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Abstract

We previously reported that the peroxisome proliferator-activated receptor γ (PPARγ) agonist rosiglitazone (RSG) improved hippocampus-dependent cognition in the Alzheimer's disease (AD) mouse model, Tg2576. RSG had no effect on wild-type littermate cognitive performance. Since extracellular signal-regulated protein kinase mitogen-activated protein kinase (ERK MAPK) is required for many forms of learning and memory that are affected in AD, and since both PPARγ and ERK MAPK are key mediators of insulin signaling, the current study tested the hypothesis that RSG-mediated cognitive improvement induces a hippocampal PPARγ pattern of gene and protein expression that converges with the ERK MAPK signaling axis in Tg2576 AD mice. In the hippocampal PPARγ transcriptome, we found significant overlap between peroxisome proliferator response element-containing PPARγ target genes and ERK-regulated, cAMP response element-containing target genes. Within the Tg2576 dentate gyrus proteome, RSG induced proteins with structural, energy, biosynthesis and plasticity functions. Several of these proteins are known to be important for cognitive function and are also regulated by ERK MAPK. In addition, we found the RSG-mediated augmentation of PPARγ and ERK2 activity during Tg2576 cognitive enhancement was reversed when hippocampal PPARγ was pharmacologically antagonized, revealing a coordinate relationship between PPARγ transcriptional competency and phosphorylated ERK that is reciprocally affected in response to chronic activation, compared with acute inhibition, of PPARγ. We conclude that the hippocampal transcriptome and proteome induced by cognitive enhancement with RSG harnesses a dysregulated ERK MAPK signal transduction pathway to overcome AD-like cognitive deficits in Tg2576 mice. Thus, PPARγ represents a signaling system that is not crucial for normal cognition yet can intercede to restore neural networks compromised by AD.

Original languageEnglish (US)
Pages (from-to)16725-16735
Number of pages11
JournalJournal of Neuroscience
Volume32
Issue number47
DOIs
StatePublished - Nov 21 2012

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rosiglitazone
Peroxisome Proliferator-Activated Receptors
Extracellular Signal-Regulated MAP Kinases
Mitogen-Activated Protein Kinases
Protein Kinases
Alzheimer Disease
Cognition
Response Elements
Proteome
Transcriptome
Peroxisome Proliferators
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

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Denner, L. A., Rodriguez-Rivera, J., Haidacher, S. J., Jahrling, J. B., Carmical, J. R., Hernandez, C. M., ... Dineley, K. T. (2012). Cognitive enhancement with rosiglitazone links the hippocampal PPARγ and ERK MAPK signaling pathways. Journal of Neuroscience, 32(47), 16725-16735. https://doi.org/10.1523/JNEUROSCI.2153-12.2012

Cognitive enhancement with rosiglitazone links the hippocampal PPARγ and ERK MAPK signaling pathways. / Denner, Larry A.; Rodriguez-Rivera, Jennifer; Haidacher, Sigmund J.; Jahrling, Jordan B.; Carmical, J. Russ; Hernandez, Caterina M.; Zhao, Yingxin; Sadygov, Rovshan G.; Starkey, Jonathan M.; Spratt, Heidi; Luxon, Bruce A.; Wood, Thomas G.; Dineley, Kelly T.

In: Journal of Neuroscience, Vol. 32, No. 47, 21.11.2012, p. 16725-16735.

Research output: Contribution to journalArticle

Denner, LA, Rodriguez-Rivera, J, Haidacher, SJ, Jahrling, JB, Carmical, JR, Hernandez, CM, Zhao, Y, Sadygov, RG, Starkey, JM, Spratt, H, Luxon, BA, Wood, TG & Dineley, KT 2012, 'Cognitive enhancement with rosiglitazone links the hippocampal PPARγ and ERK MAPK signaling pathways', Journal of Neuroscience, vol. 32, no. 47, pp. 16725-16735. https://doi.org/10.1523/JNEUROSCI.2153-12.2012
Denner LA, Rodriguez-Rivera J, Haidacher SJ, Jahrling JB, Carmical JR, Hernandez CM et al. Cognitive enhancement with rosiglitazone links the hippocampal PPARγ and ERK MAPK signaling pathways. Journal of Neuroscience. 2012 Nov 21;32(47):16725-16735. https://doi.org/10.1523/JNEUROSCI.2153-12.2012
Denner, Larry A. ; Rodriguez-Rivera, Jennifer ; Haidacher, Sigmund J. ; Jahrling, Jordan B. ; Carmical, J. Russ ; Hernandez, Caterina M. ; Zhao, Yingxin ; Sadygov, Rovshan G. ; Starkey, Jonathan M. ; Spratt, Heidi ; Luxon, Bruce A. ; Wood, Thomas G. ; Dineley, Kelly T. / Cognitive enhancement with rosiglitazone links the hippocampal PPARγ and ERK MAPK signaling pathways. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 47. pp. 16725-16735.
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