Cold-induced vasodilatation in isolated, perfused rat tail artery

C. A. Gardner, R Clinton Webb

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

This study characterizes an in vitro model of the 'hunting response' (cold-induced vasoconstriction and vasodilatation). Two-centimeter segments of rat tail arteries (n = 15) were placed in a muscle bath (37° C) and perfused (37°C) at constant pressure (50 mmHg; flow = 14.5 ± 0.8 ml/min) with physiological salt solution. Arteries constricted (23.7 ± 2.8% decrease in flow) in response to activation of adrenergic nerves by electrical stimulation (9 V, 0.1-1.0 Hz, 0.1-4 ms). Cooling the bath to 4-12° C (perfusate = 37°C) caused further flow reduction (0-0.5 ml/min) in 14 arteries. After 20-40 min, 12 arteries dilated (7.4 ± 1.2 ml/min) followed by constriction in 5-10 min. Typically, flow oscillated between periods of prolonged low flow and brief periods of high flow. Phentolamine (10-6 M in bath) and acute adrenergic denervation blocked flow changes caused by decreased bath temperature. In unstimulated arteries, exogenous norepinephrine (6 x 10-8 M in bath) decreased flow by 20%. On cooling (7-10° C) flow decreased to zero, but did not oscillate. These results are consistent with the hypothesis that cold-induced vasoconstriction is caused by augmented smooth muscle responsiveness to norepinephrine, whereas cold-induced vasodilatation is caused by a cessation of transmitter release from adrenergic nerve endings.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume251
Issue number1
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Baths
Vasodilation
Tail
Arteries
Adrenergic Agents
Vasoconstriction
Norepinephrine
Nerve Endings
Phentolamine
Denervation
Constriction
Electric Stimulation
Smooth Muscle
Salts
Pressure
Muscles
Temperature

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Cold-induced vasodilatation in isolated, perfused rat tail artery. / Gardner, C. A.; Webb, R Clinton.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 251, No. 1, 01.01.1986.

Research output: Contribution to journalArticle

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