Collagen degradation by host-derived enzymes during aging

David Henry Pashley, Franklin Chi Meng Tay, C. Yiu, M. Hashimoto, L. Breschi, R. M. Carvalho, S. Ito

Research output: Contribution to journalArticle

610 Citations (Scopus)

Abstract

Incompletely infiltrated collagen fibrils in acid-etched dentin are susceptible to degradation. We hypothesize that degradation can occur in the absence of bacteria. Partially demineralized collagen matrices (DCMs) prepared from human dentin were stored in artificial saliva. Control specimens were stored in artificial saliva containing proteolytic enzyme inhibitors, or pure mineral oil. We retrieved them at 24 hrs, 90 and 250 days to examine the extent of degradation of DCM. In the 24-hour experimental and 90- and 250-day control specimens, we observed 5- to 6-μm-thick layers of DCM containing banded collagen fibrils. DCMs were almost completely destroyed in the 250-day experimental specimens, but not when incubated with enzyme inhibitors or mineral oil. Functional enzyme analysis of dentin powder revealed low levels of collagenolytic activity that was inhibited by protease inhibitors or 0.2% chlorhexidine. We hypothesize that collagen degradation occurred over time, via host-derived matrix metalloproteinases that are released slowly over time.

Original languageEnglish (US)
Pages (from-to)216-221
Number of pages6
JournalJournal of Dental Research
Volume83
Issue number3
DOIs
StatePublished - Jan 1 2004

Fingerprint

Collagen
Enzymes
Dentin
Artificial Saliva
Mineral Oil
Enzyme Inhibitors
Chlorhexidine
Protease Inhibitors
Matrix Metalloproteinases
Powders
Peptide Hydrolases
Bacteria
Acids

Keywords

  • Dentin matrix
  • Gelatinase
  • Matrix metalloproteinases (MMPs)

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Pashley, D. H., Tay, F. C. M., Yiu, C., Hashimoto, M., Breschi, L., Carvalho, R. M., & Ito, S. (2004). Collagen degradation by host-derived enzymes during aging. Journal of Dental Research, 83(3), 216-221. https://doi.org/10.1177/154405910408300306

Collagen degradation by host-derived enzymes during aging. / Pashley, David Henry; Tay, Franklin Chi Meng; Yiu, C.; Hashimoto, M.; Breschi, L.; Carvalho, R. M.; Ito, S.

In: Journal of Dental Research, Vol. 83, No. 3, 01.01.2004, p. 216-221.

Research output: Contribution to journalArticle

Pashley, DH, Tay, FCM, Yiu, C, Hashimoto, M, Breschi, L, Carvalho, RM & Ito, S 2004, 'Collagen degradation by host-derived enzymes during aging', Journal of Dental Research, vol. 83, no. 3, pp. 216-221. https://doi.org/10.1177/154405910408300306
Pashley DH, Tay FCM, Yiu C, Hashimoto M, Breschi L, Carvalho RM et al. Collagen degradation by host-derived enzymes during aging. Journal of Dental Research. 2004 Jan 1;83(3):216-221. https://doi.org/10.1177/154405910408300306
Pashley, David Henry ; Tay, Franklin Chi Meng ; Yiu, C. ; Hashimoto, M. ; Breschi, L. ; Carvalho, R. M. ; Ito, S. / Collagen degradation by host-derived enzymes during aging. In: Journal of Dental Research. 2004 ; Vol. 83, No. 3. pp. 216-221.
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