Color transformation and fluorescence of prussian blue-positive cells: Implications for histologic verification of cells labeled with superparamagnetic iron oxide nanoparticles

Joseph A. Frank, Heather Kalish, E. Kay Jordan, Stasia A. Anderson, Edyta Pawelczyk, Ali S. Arbab

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Superparamagnetic iron oxide (SPIO) nanoparticles, either modified or in combination with other macromolecules, are being used for magnetic labeling of stem cells and other cells to monitor cell trafficking by magnetic resonance imaging (MRI) in experimental models. The correlation of histology to MRI depends on the ability to detect SPIO-labeled cells using Prussian blue (PB) stain and fluorescent tags to cell surface markers. Exposure of PB-positive sections to ultraviolet light at a wavelength of 365 nm commonly used fluorescence microscopy can result in color transformation of PB-positive material from blue to brown. Although the PB color transformation is primarily an artifact that may occur during fluorescence microscopy, the transformation can be manipulated using imaging process software for the detection of low levels of iron labeled cells in tissues samples

Original languageEnglish (US)
Pages (from-to)212-218
Number of pages7
JournalMolecular Imaging
Volume6
Issue number3
DOIs
StatePublished - May 1 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Medicine(all)
  • Biomedical Engineering
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

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