Combination of transfection agents and magnetic resonance contrast agents for cellular imaging

Relationship between relaxivities, electrostatic forces, and chemical composition

Heather Kalish, Ali Syed Arbab, B. R. Miller, B. K. Lewis, H. A. Zywicke, J. W.M. Bulte, L. H. Bryant, J. A. Frank

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The purpose of this study was to investigate the changes in electrostatic and magnetic resonance (MR) properties observed when MR contrast agents (CAs) (Feridex®, MION-46L, or G5-dendrimer-DOTA-Gd) are combined with transfection agents (TAs) under various conditions for use as a CA-TA complex basis for cellular labeling and MRI. CAs were incubated with various classes of TAs for 0-48 hr in solutions of varying concentrations and pH values. NMR relaxation rates (1/T1, 1/T2), MRI and zeta potential (ZP) of CA-TA solutions were measured. TAs decreased the 1/T1 and 1/T2 of G5-DOTA-Gd, Feridex®, and MION-46L by 0-95%. Altering the pH of G5-DOTA-Gd-TA decreased the T1-weighted signal intensity (SI) on MRI from 0 to 78%. Measured ZP values for G5-DOTA-Gd, Feridex®, and MION-46L were -51, -41, and -2.0 mV, respectively. The TA LV had a negative ZP, while the other TAs had ZPs ranging from +20 to +65 mV. The alteration of the ZP and NMR relaxivities of the MR CAs, Feridex®, MION-46L, and G5-DOTA-Gd by TAs has been demonstrated. These results enhance our understanding of the relationship between electrostatic and MR properties.

Original languageEnglish (US)
Pages (from-to)275-282
Number of pages8
JournalMagnetic Resonance in Medicine
Volume50
Issue number2
DOIs
StatePublished - Aug 1 2003

Fingerprint

Static Electricity
Contrast Media
Transfection
Magnetic Resonance Spectroscopy
Dendrimers
gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate
monocrystalline iron oxide nanoparticle
ferumoxides

Keywords

  • Contrast agent
  • MRI
  • Relaxivities
  • Transfection agents
  • Zeta potential

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Combination of transfection agents and magnetic resonance contrast agents for cellular imaging : Relationship between relaxivities, electrostatic forces, and chemical composition. / Kalish, Heather; Arbab, Ali Syed; Miller, B. R.; Lewis, B. K.; Zywicke, H. A.; Bulte, J. W.M.; Bryant, L. H.; Frank, J. A.

In: Magnetic Resonance in Medicine, Vol. 50, No. 2, 01.08.2003, p. 275-282.

Research output: Contribution to journalArticle

Kalish, Heather ; Arbab, Ali Syed ; Miller, B. R. ; Lewis, B. K. ; Zywicke, H. A. ; Bulte, J. W.M. ; Bryant, L. H. ; Frank, J. A. / Combination of transfection agents and magnetic resonance contrast agents for cellular imaging : Relationship between relaxivities, electrostatic forces, and chemical composition. In: Magnetic Resonance in Medicine. 2003 ; Vol. 50, No. 2. pp. 275-282.
@article{c85d7600acc64ec38a62324cfda003fa,
title = "Combination of transfection agents and magnetic resonance contrast agents for cellular imaging: Relationship between relaxivities, electrostatic forces, and chemical composition",
abstract = "The purpose of this study was to investigate the changes in electrostatic and magnetic resonance (MR) properties observed when MR contrast agents (CAs) (Feridex{\circledR}, MION-46L, or G5-dendrimer-DOTA-Gd) are combined with transfection agents (TAs) under various conditions for use as a CA-TA complex basis for cellular labeling and MRI. CAs were incubated with various classes of TAs for 0-48 hr in solutions of varying concentrations and pH values. NMR relaxation rates (1/T1, 1/T2), MRI and zeta potential (ZP) of CA-TA solutions were measured. TAs decreased the 1/T1 and 1/T2 of G5-DOTA-Gd, Feridex{\circledR}, and MION-46L by 0-95{\%}. Altering the pH of G5-DOTA-Gd-TA decreased the T1-weighted signal intensity (SI) on MRI from 0 to 78{\%}. Measured ZP values for G5-DOTA-Gd, Feridex{\circledR}, and MION-46L were -51, -41, and -2.0 mV, respectively. The TA LV had a negative ZP, while the other TAs had ZPs ranging from +20 to +65 mV. The alteration of the ZP and NMR relaxivities of the MR CAs, Feridex{\circledR}, MION-46L, and G5-DOTA-Gd by TAs has been demonstrated. These results enhance our understanding of the relationship between electrostatic and MR properties.",
keywords = "Contrast agent, MRI, Relaxivities, Transfection agents, Zeta potential",
author = "Heather Kalish and Arbab, {Ali Syed} and Miller, {B. R.} and Lewis, {B. K.} and Zywicke, {H. A.} and Bulte, {J. W.M.} and Bryant, {L. H.} and Frank, {J. A.}",
year = "2003",
month = "8",
day = "1",
doi = "10.1002/mrm.10556",
language = "English (US)",
volume = "50",
pages = "275--282",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - Combination of transfection agents and magnetic resonance contrast agents for cellular imaging

T2 - Relationship between relaxivities, electrostatic forces, and chemical composition

AU - Kalish, Heather

AU - Arbab, Ali Syed

AU - Miller, B. R.

AU - Lewis, B. K.

AU - Zywicke, H. A.

AU - Bulte, J. W.M.

AU - Bryant, L. H.

AU - Frank, J. A.

PY - 2003/8/1

Y1 - 2003/8/1

N2 - The purpose of this study was to investigate the changes in electrostatic and magnetic resonance (MR) properties observed when MR contrast agents (CAs) (Feridex®, MION-46L, or G5-dendrimer-DOTA-Gd) are combined with transfection agents (TAs) under various conditions for use as a CA-TA complex basis for cellular labeling and MRI. CAs were incubated with various classes of TAs for 0-48 hr in solutions of varying concentrations and pH values. NMR relaxation rates (1/T1, 1/T2), MRI and zeta potential (ZP) of CA-TA solutions were measured. TAs decreased the 1/T1 and 1/T2 of G5-DOTA-Gd, Feridex®, and MION-46L by 0-95%. Altering the pH of G5-DOTA-Gd-TA decreased the T1-weighted signal intensity (SI) on MRI from 0 to 78%. Measured ZP values for G5-DOTA-Gd, Feridex®, and MION-46L were -51, -41, and -2.0 mV, respectively. The TA LV had a negative ZP, while the other TAs had ZPs ranging from +20 to +65 mV. The alteration of the ZP and NMR relaxivities of the MR CAs, Feridex®, MION-46L, and G5-DOTA-Gd by TAs has been demonstrated. These results enhance our understanding of the relationship between electrostatic and MR properties.

AB - The purpose of this study was to investigate the changes in electrostatic and magnetic resonance (MR) properties observed when MR contrast agents (CAs) (Feridex®, MION-46L, or G5-dendrimer-DOTA-Gd) are combined with transfection agents (TAs) under various conditions for use as a CA-TA complex basis for cellular labeling and MRI. CAs were incubated with various classes of TAs for 0-48 hr in solutions of varying concentrations and pH values. NMR relaxation rates (1/T1, 1/T2), MRI and zeta potential (ZP) of CA-TA solutions were measured. TAs decreased the 1/T1 and 1/T2 of G5-DOTA-Gd, Feridex®, and MION-46L by 0-95%. Altering the pH of G5-DOTA-Gd-TA decreased the T1-weighted signal intensity (SI) on MRI from 0 to 78%. Measured ZP values for G5-DOTA-Gd, Feridex®, and MION-46L were -51, -41, and -2.0 mV, respectively. The TA LV had a negative ZP, while the other TAs had ZPs ranging from +20 to +65 mV. The alteration of the ZP and NMR relaxivities of the MR CAs, Feridex®, MION-46L, and G5-DOTA-Gd by TAs has been demonstrated. These results enhance our understanding of the relationship between electrostatic and MR properties.

KW - Contrast agent

KW - MRI

KW - Relaxivities

KW - Transfection agents

KW - Zeta potential

UR - http://www.scopus.com/inward/record.url?scp=0042092068&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042092068&partnerID=8YFLogxK

U2 - 10.1002/mrm.10556

DO - 10.1002/mrm.10556

M3 - Article

VL - 50

SP - 275

EP - 282

JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

SN - 0740-3194

IS - 2

ER -