Combinatorial treatment of bone marrow stem cells and stromal cell-derived factor 1 improves glycemia and insulin production in diabetic mice

H. Cheng, Y. C. Zhang, S. Wolfe, V. Valencia, K. Qian, L. Shen, Y. L. Tang, W. H. Hsu, M. A. Atkinson, M. I. Phillips

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Transdifferentiation of stem cells into insulin-producing cells for the treatment of diabetes have shown promising but inconsistent results. We examined the potential for attracting bone marrow stem cells (BMSCs) to the pancreas using a chemokine, stromal cell-derived factor 1 (SDF-1). SDF-1 treatment markedly increased the number of GFP labeled BMSCs in the pancreas, but surprisingly, the majority was observed in liver. The liver cells had typical pancreatic endocrine cell gene expression including insulin I, insulin II, PDX-1, somatostatin, and glucagon. Combined treatment with SDF-1 and BMSC transplant reduced hyperglycemia and prolonged the long-term survival of diabetic mice, and a sub group had complete normoglycemia (<150. mg/dl), restored blood insulin levels, and normal glucose tolerance. Our results suggest that SDF-1 could potentially be used to improve the homing of stem cells and β-cell regeneration. The mechanism appears to involve an increase in insulin producing cells mainly in the liver.

Original languageEnglish (US)
Pages (from-to)88-96
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume345
Issue number1-2
DOIs
StatePublished - Oct 15 2011
Externally publishedYes

Keywords

  • Bone marrow stem cells
  • Diabetes
  • Homing
  • Insulin
  • Liver
  • SDF-1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Fingerprint Dive into the research topics of 'Combinatorial treatment of bone marrow stem cells and stromal cell-derived factor 1 improves glycemia and insulin production in diabetic mice'. Together they form a unique fingerprint.

  • Cite this