Combinatorial treatment of bone marrow stem cells and stromal cell-derived factor 1 improves glycemia and insulin production in diabetic mice

H. Cheng, Y. C. Zhang, S. Wolfe, V. Valencia, K. Qian, L. Shen, Yao Liang Tang, W. H. Hsu, M. A. Atkinson, M. I. Phillips

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Transdifferentiation of stem cells into insulin-producing cells for the treatment of diabetes have shown promising but inconsistent results. We examined the potential for attracting bone marrow stem cells (BMSCs) to the pancreas using a chemokine, stromal cell-derived factor 1 (SDF-1). SDF-1 treatment markedly increased the number of GFP labeled BMSCs in the pancreas, but surprisingly, the majority was observed in liver. The liver cells had typical pancreatic endocrine cell gene expression including insulin I, insulin II, PDX-1, somatostatin, and glucagon. Combined treatment with SDF-1 and BMSC transplant reduced hyperglycemia and prolonged the long-term survival of diabetic mice, and a sub group had complete normoglycemia (<150. mg/dl), restored blood insulin levels, and normal glucose tolerance. Our results suggest that SDF-1 could potentially be used to improve the homing of stem cells and β-cell regeneration. The mechanism appears to involve an increase in insulin producing cells mainly in the liver.

Original languageEnglish (US)
Pages (from-to)88-96
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume345
Issue number1-2
DOIs
StatePublished - Oct 15 2011

Fingerprint

Chemokine CXCL12
Stem cells
Bone Marrow Cells
Bone
Stem Cells
Insulin
Liver
Pancreas
Transplants
Endocrine Cells
Medical problems
Somatostatin
Glucagon
Chemokines
Gene expression
Hyperglycemia
Regeneration
Blood
Cells
Gene Expression

Keywords

  • Bone marrow stem cells
  • Diabetes
  • Homing
  • Insulin
  • Liver
  • SDF-1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Combinatorial treatment of bone marrow stem cells and stromal cell-derived factor 1 improves glycemia and insulin production in diabetic mice. / Cheng, H.; Zhang, Y. C.; Wolfe, S.; Valencia, V.; Qian, K.; Shen, L.; Tang, Yao Liang; Hsu, W. H.; Atkinson, M. A.; Phillips, M. I.

In: Molecular and Cellular Endocrinology, Vol. 345, No. 1-2, 15.10.2011, p. 88-96.

Research output: Contribution to journalArticle

Cheng, H. ; Zhang, Y. C. ; Wolfe, S. ; Valencia, V. ; Qian, K. ; Shen, L. ; Tang, Yao Liang ; Hsu, W. H. ; Atkinson, M. A. ; Phillips, M. I. / Combinatorial treatment of bone marrow stem cells and stromal cell-derived factor 1 improves glycemia and insulin production in diabetic mice. In: Molecular and Cellular Endocrinology. 2011 ; Vol. 345, No. 1-2. pp. 88-96.
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