Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons

Min Deng, Hua Yang, Xiaoling Xie, Guoqing Liang, Lin Gan

Research output: Contribution to journalArticle

Abstract

POU-homeodomain and LIM-homeodomain transcription factors are expressed in developing projection neurons within retina, inner ear, dorsal root ganglion, and trigeminal ganglion, and play synergistic roles in their differentiation and survival. Here, using immunohistochemistry, we present a comparative analysis of the spatiotemporal expression pattern of POU4F1, POU4F2, and ISL1 during the development of cochleovestibular ganglion (CVG) neurons in mouse inner ear. At early stages, when otic neurons are first detected in the otic epithelium (OE) and migrate into periotic mesenchyme to form the CVG, POU4F1 and ISL1 are co-expressed in a majority of the delaminated CVG neurons, which are marked by NEUROD1 expression, but POU4F1 is absent in the otic epithelium. The onset of POU4F2 expression starts after that of POU4F1 and ISL1, and is observed in the NEUROD1-negative, post-mitotic CVG neurons. When the CVG neurons innervate the vestibular and cochlear sensory organs, the expression of POU4F1, POU4F2, and ISL1 continues in both vestibular and spiral ganglion cells. Later in development, POU4F1 expression becomes down-regulated in a majority of spiral ganglion (SG) neurons and more neurons express POU4F2 expression while ISL1 expression is maintained. The differential as well as overlapping expression of POU4F1, POU4F2, and ISL1 combined with previous studies suggests possible functional interaction and regulatory relationship of these transcription factors in the development of inner ear neurons.

Original languageEnglish (US)
Pages (from-to)31-37
Number of pages7
JournalGene Expression Patterns
Volume15
Issue number1
DOIs
StatePublished - May 2014
Externally publishedYes

Fingerprint

Ganglia
Neurons
Inner Ear
Spiral Ganglion
Ear
Transcription Factors
Epithelium
Spatio-Temporal Analysis
Trigeminal Ganglion
Cochlea
Spinal Ganglia
Mesoderm
Retina
Immunohistochemistry

Keywords

  • CVG
  • Inner ear
  • LIM-homeodomain
  • Neurogenesis
  • POU-homeodomain
  • Transcription factor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Developmental Biology

Cite this

Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons. / Deng, Min; Yang, Hua; Xie, Xiaoling; Liang, Guoqing; Gan, Lin.

In: Gene Expression Patterns, Vol. 15, No. 1, 05.2014, p. 31-37.

Research output: Contribution to journalArticle

Deng, Min ; Yang, Hua ; Xie, Xiaoling ; Liang, Guoqing ; Gan, Lin. / Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons. In: Gene Expression Patterns. 2014 ; Vol. 15, No. 1. pp. 31-37.
@article{17cc67e5f8dd425b9e4ea04fe1046daa,
title = "Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons",
abstract = "POU-homeodomain and LIM-homeodomain transcription factors are expressed in developing projection neurons within retina, inner ear, dorsal root ganglion, and trigeminal ganglion, and play synergistic roles in their differentiation and survival. Here, using immunohistochemistry, we present a comparative analysis of the spatiotemporal expression pattern of POU4F1, POU4F2, and ISL1 during the development of cochleovestibular ganglion (CVG) neurons in mouse inner ear. At early stages, when otic neurons are first detected in the otic epithelium (OE) and migrate into periotic mesenchyme to form the CVG, POU4F1 and ISL1 are co-expressed in a majority of the delaminated CVG neurons, which are marked by NEUROD1 expression, but POU4F1 is absent in the otic epithelium. The onset of POU4F2 expression starts after that of POU4F1 and ISL1, and is observed in the NEUROD1-negative, post-mitotic CVG neurons. When the CVG neurons innervate the vestibular and cochlear sensory organs, the expression of POU4F1, POU4F2, and ISL1 continues in both vestibular and spiral ganglion cells. Later in development, POU4F1 expression becomes down-regulated in a majority of spiral ganglion (SG) neurons and more neurons express POU4F2 expression while ISL1 expression is maintained. The differential as well as overlapping expression of POU4F1, POU4F2, and ISL1 combined with previous studies suggests possible functional interaction and regulatory relationship of these transcription factors in the development of inner ear neurons.",
keywords = "CVG, Inner ear, LIM-homeodomain, Neurogenesis, POU-homeodomain, Transcription factor",
author = "Min Deng and Hua Yang and Xiaoling Xie and Guoqing Liang and Lin Gan",
year = "2014",
month = "5",
doi = "10.1016/j.gep.2014.03.001",
language = "English (US)",
volume = "15",
pages = "31--37",
journal = "Brain research. Gene expression patterns",
issn = "1567-133X",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons

AU - Deng, Min

AU - Yang, Hua

AU - Xie, Xiaoling

AU - Liang, Guoqing

AU - Gan, Lin

PY - 2014/5

Y1 - 2014/5

N2 - POU-homeodomain and LIM-homeodomain transcription factors are expressed in developing projection neurons within retina, inner ear, dorsal root ganglion, and trigeminal ganglion, and play synergistic roles in their differentiation and survival. Here, using immunohistochemistry, we present a comparative analysis of the spatiotemporal expression pattern of POU4F1, POU4F2, and ISL1 during the development of cochleovestibular ganglion (CVG) neurons in mouse inner ear. At early stages, when otic neurons are first detected in the otic epithelium (OE) and migrate into periotic mesenchyme to form the CVG, POU4F1 and ISL1 are co-expressed in a majority of the delaminated CVG neurons, which are marked by NEUROD1 expression, but POU4F1 is absent in the otic epithelium. The onset of POU4F2 expression starts after that of POU4F1 and ISL1, and is observed in the NEUROD1-negative, post-mitotic CVG neurons. When the CVG neurons innervate the vestibular and cochlear sensory organs, the expression of POU4F1, POU4F2, and ISL1 continues in both vestibular and spiral ganglion cells. Later in development, POU4F1 expression becomes down-regulated in a majority of spiral ganglion (SG) neurons and more neurons express POU4F2 expression while ISL1 expression is maintained. The differential as well as overlapping expression of POU4F1, POU4F2, and ISL1 combined with previous studies suggests possible functional interaction and regulatory relationship of these transcription factors in the development of inner ear neurons.

AB - POU-homeodomain and LIM-homeodomain transcription factors are expressed in developing projection neurons within retina, inner ear, dorsal root ganglion, and trigeminal ganglion, and play synergistic roles in their differentiation and survival. Here, using immunohistochemistry, we present a comparative analysis of the spatiotemporal expression pattern of POU4F1, POU4F2, and ISL1 during the development of cochleovestibular ganglion (CVG) neurons in mouse inner ear. At early stages, when otic neurons are first detected in the otic epithelium (OE) and migrate into periotic mesenchyme to form the CVG, POU4F1 and ISL1 are co-expressed in a majority of the delaminated CVG neurons, which are marked by NEUROD1 expression, but POU4F1 is absent in the otic epithelium. The onset of POU4F2 expression starts after that of POU4F1 and ISL1, and is observed in the NEUROD1-negative, post-mitotic CVG neurons. When the CVG neurons innervate the vestibular and cochlear sensory organs, the expression of POU4F1, POU4F2, and ISL1 continues in both vestibular and spiral ganglion cells. Later in development, POU4F1 expression becomes down-regulated in a majority of spiral ganglion (SG) neurons and more neurons express POU4F2 expression while ISL1 expression is maintained. The differential as well as overlapping expression of POU4F1, POU4F2, and ISL1 combined with previous studies suggests possible functional interaction and regulatory relationship of these transcription factors in the development of inner ear neurons.

KW - CVG

KW - Inner ear

KW - LIM-homeodomain

KW - Neurogenesis

KW - POU-homeodomain

KW - Transcription factor

UR - http://www.scopus.com/inward/record.url?scp=84899482657&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899482657&partnerID=8YFLogxK

U2 - 10.1016/j.gep.2014.03.001

DO - 10.1016/j.gep.2014.03.001

M3 - Article

C2 - 24709358

AN - SCOPUS:84899482657

VL - 15

SP - 31

EP - 37

JO - Brain research. Gene expression patterns

JF - Brain research. Gene expression patterns

SN - 1567-133X

IS - 1

ER -