Comparative genomic sequence analysis and isolation of human and mouse alternative EGFR transcripts encoding truncated receptor isoforms

Jill L. Reiter, David W. Threadgill, Greg D. Eley, Karen E. Strunk, Andrew J. Danielsen, Colleen Schehl Sinclair, R. Scott Pearsall, Patricia J. Green, Della Yee, Andrea L. Lampland, Swarna Balasubramaniam, Tonia D. Crossley, Terry R. Magnuson, C. David James, Nita J. Maihle

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

This study presents the annotated genomic sequence and exon-intron organization of the human and mouse epidermal growth factor receptor (EGFR) genes located on chromosomes 7p11.2 and 11, respectively. We report that the EGFR gene spans nearly 200 kb and that the full-length 170-kDa EGFR is encoded by 28 exons. In addition, we have identified two human and two mouse alternative EGFR transcripts of 2.4-3.0 kb using both computational and experimental methods. The human 3.0-kb and mouse 2.8-kb EGFR mRNAs are predominantly expressed in placenta and liver, respectively, and both transcripts encode 110-kDa truncated receptor isoforms containing only the extracellular ligand-binding domain. We also have demonstrated that the aberrant 2.8-kb EGFR transcript produced by the human A431 carcinoma cell line is generated by splicing to a recombinant 3′-terminal exon located in EGFR intron 16, which apparently was formed as a result of a chromosomal translocation. Finally, we have shown that the human, mouse, rat, and chicken 1.8- to 3.0-kb alternative EGFR transcripts are generated by distinct splicing mechanisms and that each of these mRNAs contains unique 3′ sequences that are not evolutionarily conserved. The presence of truncated receptor isoforms in diverse species suggests that these proteins may have important functional roles in regulating EGFR activity.

Original languageEnglish (US)
Pages (from-to)1-20
Number of pages20
JournalGenomics
Volume71
Issue number1
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Reiter, J. L., Threadgill, D. W., Eley, G. D., Strunk, K. E., Danielsen, A. J., Schehl Sinclair, C., Pearsall, R. S., Green, P. J., Yee, D., Lampland, A. L., Balasubramaniam, S., Crossley, T. D., Magnuson, T. R., James, C. D., & Maihle, N. J. (2001). Comparative genomic sequence analysis and isolation of human and mouse alternative EGFR transcripts encoding truncated receptor isoforms. Genomics, 71(1), 1-20. https://doi.org/10.1006/geno.2000.6341