Comparative SEM and TEM observations of nanoleakage within the hybrid layer.

H. Sano, M. Yoshiyama, S. Ebisu, M. F. Burrow, T. Takatsu, B. Ciucchi, R. Carvalho, David Henry Pashley

Research output: Contribution to journalArticle

224 Citations (Scopus)

Abstract

Most adhesive interface studies have involved SEM demonstration of the penetration of adhesive resins into demineralized dentin surfaces with subsequent creation of hybrid layers. Nanoleakage is a term that describes the diffusion of small ions or molecules within the hybrid layer in the absence of gap formation. The present microscopic study examined the nanoleakage of the hybrid layer using a silver nitrate staining technique. Adhesive dentin sandwiches, which were immersed in a silver nitrate solution, were prepared for both SEM and TEM examination using both the Clearfil Liner Bond and All-Bond 2 adhesive systems. Both systems demonstrated silver accumulation within the hybrid layers. Clearfil Liner Bond System showed scattered silver particles at the bottom two-thirds of the hybrid layer by both SEM and TEM observation, whereas All-Bond 2 revealed stained fiber-like structures within the full thickness of the hybrid layer. To evaluate the quality of the hybrid layer, the utilization of tracer molecules such as silver nitrate that are detectable by both SEM and TEM is proposed. It is important to determine the location and morphology of these nanometer-sized porosities that may permit the hydrolysis of collagen fibers and degradation of adhesive monomers.

Original languageEnglish (US)
Pages (from-to)160-167
Number of pages8
JournalOperative Dentistry
Volume20
Issue number4
StatePublished - Jan 1 1995

Fingerprint

Adhesives
Silver Nitrate
Dentin
Silver
Silver Staining
Porosity
Hydrolysis
Collagen
Observation
Ions
All-Bond 2

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Sano, H., Yoshiyama, M., Ebisu, S., Burrow, M. F., Takatsu, T., Ciucchi, B., ... Pashley, D. H. (1995). Comparative SEM and TEM observations of nanoleakage within the hybrid layer. Operative Dentistry, 20(4), 160-167.

Comparative SEM and TEM observations of nanoleakage within the hybrid layer. / Sano, H.; Yoshiyama, M.; Ebisu, S.; Burrow, M. F.; Takatsu, T.; Ciucchi, B.; Carvalho, R.; Pashley, David Henry.

In: Operative Dentistry, Vol. 20, No. 4, 01.01.1995, p. 160-167.

Research output: Contribution to journalArticle

Sano, H, Yoshiyama, M, Ebisu, S, Burrow, MF, Takatsu, T, Ciucchi, B, Carvalho, R & Pashley, DH 1995, 'Comparative SEM and TEM observations of nanoleakage within the hybrid layer.', Operative Dentistry, vol. 20, no. 4, pp. 160-167.
Sano H, Yoshiyama M, Ebisu S, Burrow MF, Takatsu T, Ciucchi B et al. Comparative SEM and TEM observations of nanoleakage within the hybrid layer. Operative Dentistry. 1995 Jan 1;20(4):160-167.
Sano, H. ; Yoshiyama, M. ; Ebisu, S. ; Burrow, M. F. ; Takatsu, T. ; Ciucchi, B. ; Carvalho, R. ; Pashley, David Henry. / Comparative SEM and TEM observations of nanoleakage within the hybrid layer. In: Operative Dentistry. 1995 ; Vol. 20, No. 4. pp. 160-167.
@article{0b4cb1fc8f134dcfa3faa35e02d7b173,
title = "Comparative SEM and TEM observations of nanoleakage within the hybrid layer.",
abstract = "Most adhesive interface studies have involved SEM demonstration of the penetration of adhesive resins into demineralized dentin surfaces with subsequent creation of hybrid layers. Nanoleakage is a term that describes the diffusion of small ions or molecules within the hybrid layer in the absence of gap formation. The present microscopic study examined the nanoleakage of the hybrid layer using a silver nitrate staining technique. Adhesive dentin sandwiches, which were immersed in a silver nitrate solution, were prepared for both SEM and TEM examination using both the Clearfil Liner Bond and All-Bond 2 adhesive systems. Both systems demonstrated silver accumulation within the hybrid layers. Clearfil Liner Bond System showed scattered silver particles at the bottom two-thirds of the hybrid layer by both SEM and TEM observation, whereas All-Bond 2 revealed stained fiber-like structures within the full thickness of the hybrid layer. To evaluate the quality of the hybrid layer, the utilization of tracer molecules such as silver nitrate that are detectable by both SEM and TEM is proposed. It is important to determine the location and morphology of these nanometer-sized porosities that may permit the hydrolysis of collagen fibers and degradation of adhesive monomers.",
author = "H. Sano and M. Yoshiyama and S. Ebisu and Burrow, {M. F.} and T. Takatsu and B. Ciucchi and R. Carvalho and Pashley, {David Henry}",
year = "1995",
month = "1",
day = "1",
language = "English (US)",
volume = "20",
pages = "160--167",
journal = "Operative Dentistry",
issn = "0361-7734",
publisher = "Indiana University School of Dentistry",
number = "4",

}

TY - JOUR

T1 - Comparative SEM and TEM observations of nanoleakage within the hybrid layer.

AU - Sano, H.

AU - Yoshiyama, M.

AU - Ebisu, S.

AU - Burrow, M. F.

AU - Takatsu, T.

AU - Ciucchi, B.

AU - Carvalho, R.

AU - Pashley, David Henry

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Most adhesive interface studies have involved SEM demonstration of the penetration of adhesive resins into demineralized dentin surfaces with subsequent creation of hybrid layers. Nanoleakage is a term that describes the diffusion of small ions or molecules within the hybrid layer in the absence of gap formation. The present microscopic study examined the nanoleakage of the hybrid layer using a silver nitrate staining technique. Adhesive dentin sandwiches, which were immersed in a silver nitrate solution, were prepared for both SEM and TEM examination using both the Clearfil Liner Bond and All-Bond 2 adhesive systems. Both systems demonstrated silver accumulation within the hybrid layers. Clearfil Liner Bond System showed scattered silver particles at the bottom two-thirds of the hybrid layer by both SEM and TEM observation, whereas All-Bond 2 revealed stained fiber-like structures within the full thickness of the hybrid layer. To evaluate the quality of the hybrid layer, the utilization of tracer molecules such as silver nitrate that are detectable by both SEM and TEM is proposed. It is important to determine the location and morphology of these nanometer-sized porosities that may permit the hydrolysis of collagen fibers and degradation of adhesive monomers.

AB - Most adhesive interface studies have involved SEM demonstration of the penetration of adhesive resins into demineralized dentin surfaces with subsequent creation of hybrid layers. Nanoleakage is a term that describes the diffusion of small ions or molecules within the hybrid layer in the absence of gap formation. The present microscopic study examined the nanoleakage of the hybrid layer using a silver nitrate staining technique. Adhesive dentin sandwiches, which were immersed in a silver nitrate solution, were prepared for both SEM and TEM examination using both the Clearfil Liner Bond and All-Bond 2 adhesive systems. Both systems demonstrated silver accumulation within the hybrid layers. Clearfil Liner Bond System showed scattered silver particles at the bottom two-thirds of the hybrid layer by both SEM and TEM observation, whereas All-Bond 2 revealed stained fiber-like structures within the full thickness of the hybrid layer. To evaluate the quality of the hybrid layer, the utilization of tracer molecules such as silver nitrate that are detectable by both SEM and TEM is proposed. It is important to determine the location and morphology of these nanometer-sized porosities that may permit the hydrolysis of collagen fibers and degradation of adhesive monomers.

UR - http://www.scopus.com/inward/record.url?scp=0029330646&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029330646&partnerID=8YFLogxK

M3 - Article

VL - 20

SP - 160

EP - 167

JO - Operative Dentistry

JF - Operative Dentistry

SN - 0361-7734

IS - 4

ER -