Comparative study of Chinese hamster ovary cell versus escherichia coli-derived bone morphogenetic protein-2 using the critical-size supraalveolar peri-implant defect model

Jaebum Lee, Eui Nam Lee, James Yoon, Sung Min Chung, Hari Prasad, Cristiano Susin, Ulf M E Wikesjö

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Background: Chinese hamster ovary (CHO) cell-derived recombinant human bone morphogenetic protein-2 (rhBMP-2) has been introduced for spine, long bone, and craniofacial indications. Escherichia coli- (E. coli) derived rhBMP-2 displays comparable efficacy to CHO cell-derived rhBMP-2 in vitro and in small-animal models. The objective of this study is to evaluate the efficacy of E. coli-derived rhBMP-2 compared to the benchmark CHO cell-derived rhBMP-2 using an established large-animal model. Methods: Contralateral, critical-size supraalveolar peri-implant defects in six adult male Hound Labrador mongrel dogs received CHOcell- or E. coli-derived rhBMP-2 (0.2 mg/mL) in an absorbable collagen sponge (ACS) carrier. In each quadrant, three dental implants were placed. A titaniummesh device was used to support space provision. The animals received fluorescent bone markers for qualitative evaluations. Animals were euthanized at 8 weeks for histopathologic and histometric evaluation. Results: Clinical healing included significant swelling, but none of the animals experienced wound dehiscences. CHO cell- and E. coli-derived rhBMP-2 supported comparable bone formation (new bone area, 35.8 ± 3.6 versus 30.1 ± 2.2 mm2; bone density, 31.8% ± 1.6% versus 35.6% ± 2.5%; and osseointegration, 32.9% ± 7.4% versus 33.7% ± 8.1%) without statistically significant differences between treatments. Newly formed immature delicate trabecular bone in fibrovascular marrow filled the space underneath the titanium mesh and extended coronally above the mesh. Seroma formation was frequently observed. There were no discernable qualitative histologic differences between treatments. Conclusion: CHO cell- and E. coli-derived rhBMP-2 in an ACS carrier appear equally effective at inducing local bone formation in support of dental implant osseointegration.

Original languageEnglish (US)
Pages (from-to)415-422
Number of pages8
JournalJournal of periodontology
Volume84
Issue number3
DOIs
StatePublished - Mar 1 2013

Keywords

  • Alveolar ridge augmentation
  • Bone morphogenetic protein 2
  • CHO cells
  • Dental implants
  • Escherichia coli
  • Growth differentiation factors

ASJC Scopus subject areas

  • Periodontics

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