Abstract
Background: Chinese hamster ovary (CHO) cell-derived recombinant human bone morphogenetic protein-2 (rhBMP-2) has been introduced for spine, long bone, and craniofacial indications. Escherichia coli- (E. coli) derived rhBMP-2 displays comparable efficacy to CHO cell-derived rhBMP-2 in vitro and in small-animal models. The objective of this study is to evaluate the efficacy of E. coli-derived rhBMP-2 compared to the benchmark CHO cell-derived rhBMP-2 using an established large-animal model. Methods: Contralateral, critical-size supraalveolar peri-implant defects in six adult male Hound Labrador mongrel dogs received CHOcell- or E. coli-derived rhBMP-2 (0.2 mg/mL) in an absorbable collagen sponge (ACS) carrier. In each quadrant, three dental implants were placed. A titaniummesh device was used to support space provision. The animals received fluorescent bone markers for qualitative evaluations. Animals were euthanized at 8 weeks for histopathologic and histometric evaluation. Results: Clinical healing included significant swelling, but none of the animals experienced wound dehiscences. CHO cell- and E. coli-derived rhBMP-2 supported comparable bone formation (new bone area, 35.8 ± 3.6 versus 30.1 ± 2.2 mm2; bone density, 31.8% ± 1.6% versus 35.6% ± 2.5%; and osseointegration, 32.9% ± 7.4% versus 33.7% ± 8.1%) without statistically significant differences between treatments. Newly formed immature delicate trabecular bone in fibrovascular marrow filled the space underneath the titanium mesh and extended coronally above the mesh. Seroma formation was frequently observed. There were no discernable qualitative histologic differences between treatments. Conclusion: CHO cell- and E. coli-derived rhBMP-2 in an ACS carrier appear equally effective at inducing local bone formation in support of dental implant osseointegration.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 415-422 |
| Number of pages | 8 |
| Journal | Journal of periodontology |
| Volume | 84 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1 2013 |
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Keywords
- Alveolar ridge augmentation
- Bone morphogenetic protein 2
- CHO cells
- Dental implants
- Escherichia coli
- Growth differentiation factors
ASJC Scopus subject areas
- Periodontics
Cite this
Comparative study of Chinese hamster ovary cell versus escherichia coli-derived bone morphogenetic protein-2 using the critical-size supraalveolar peri-implant defect model. / Lee, Jaebum; Lee, Eui Nam; Yoon, James; Chung, Sung Min; Prasad, Hari; Susin, Cristiano; Wikesjö, Ulf M E.
In: Journal of periodontology, Vol. 84, No. 3, 01.03.2013, p. 415-422.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative study of Chinese hamster ovary cell versus escherichia coli-derived bone morphogenetic protein-2 using the critical-size supraalveolar peri-implant defect model
AU - Lee, Jaebum
AU - Lee, Eui Nam
AU - Yoon, James
AU - Chung, Sung Min
AU - Prasad, Hari
AU - Susin, Cristiano
AU - Wikesjö, Ulf M E
PY - 2013/3/1
Y1 - 2013/3/1
N2 - Background: Chinese hamster ovary (CHO) cell-derived recombinant human bone morphogenetic protein-2 (rhBMP-2) has been introduced for spine, long bone, and craniofacial indications. Escherichia coli- (E. coli) derived rhBMP-2 displays comparable efficacy to CHO cell-derived rhBMP-2 in vitro and in small-animal models. The objective of this study is to evaluate the efficacy of E. coli-derived rhBMP-2 compared to the benchmark CHO cell-derived rhBMP-2 using an established large-animal model. Methods: Contralateral, critical-size supraalveolar peri-implant defects in six adult male Hound Labrador mongrel dogs received CHOcell- or E. coli-derived rhBMP-2 (0.2 mg/mL) in an absorbable collagen sponge (ACS) carrier. In each quadrant, three dental implants were placed. A titaniummesh device was used to support space provision. The animals received fluorescent bone markers for qualitative evaluations. Animals were euthanized at 8 weeks for histopathologic and histometric evaluation. Results: Clinical healing included significant swelling, but none of the animals experienced wound dehiscences. CHO cell- and E. coli-derived rhBMP-2 supported comparable bone formation (new bone area, 35.8 ± 3.6 versus 30.1 ± 2.2 mm2; bone density, 31.8% ± 1.6% versus 35.6% ± 2.5%; and osseointegration, 32.9% ± 7.4% versus 33.7% ± 8.1%) without statistically significant differences between treatments. Newly formed immature delicate trabecular bone in fibrovascular marrow filled the space underneath the titanium mesh and extended coronally above the mesh. Seroma formation was frequently observed. There were no discernable qualitative histologic differences between treatments. Conclusion: CHO cell- and E. coli-derived rhBMP-2 in an ACS carrier appear equally effective at inducing local bone formation in support of dental implant osseointegration.
AB - Background: Chinese hamster ovary (CHO) cell-derived recombinant human bone morphogenetic protein-2 (rhBMP-2) has been introduced for spine, long bone, and craniofacial indications. Escherichia coli- (E. coli) derived rhBMP-2 displays comparable efficacy to CHO cell-derived rhBMP-2 in vitro and in small-animal models. The objective of this study is to evaluate the efficacy of E. coli-derived rhBMP-2 compared to the benchmark CHO cell-derived rhBMP-2 using an established large-animal model. Methods: Contralateral, critical-size supraalveolar peri-implant defects in six adult male Hound Labrador mongrel dogs received CHOcell- or E. coli-derived rhBMP-2 (0.2 mg/mL) in an absorbable collagen sponge (ACS) carrier. In each quadrant, three dental implants were placed. A titaniummesh device was used to support space provision. The animals received fluorescent bone markers for qualitative evaluations. Animals were euthanized at 8 weeks for histopathologic and histometric evaluation. Results: Clinical healing included significant swelling, but none of the animals experienced wound dehiscences. CHO cell- and E. coli-derived rhBMP-2 supported comparable bone formation (new bone area, 35.8 ± 3.6 versus 30.1 ± 2.2 mm2; bone density, 31.8% ± 1.6% versus 35.6% ± 2.5%; and osseointegration, 32.9% ± 7.4% versus 33.7% ± 8.1%) without statistically significant differences between treatments. Newly formed immature delicate trabecular bone in fibrovascular marrow filled the space underneath the titanium mesh and extended coronally above the mesh. Seroma formation was frequently observed. There were no discernable qualitative histologic differences between treatments. Conclusion: CHO cell- and E. coli-derived rhBMP-2 in an ACS carrier appear equally effective at inducing local bone formation in support of dental implant osseointegration.
KW - Alveolar ridge augmentation
KW - Bone morphogenetic protein 2
KW - CHO cells
KW - Dental implants
KW - Escherichia coli
KW - Growth differentiation factors
UR - http://www.scopus.com/inward/record.url?scp=84874614050&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874614050&partnerID=8YFLogxK
U2 - 10.1902/jop.2012.110369
DO - 10.1902/jop.2012.110369
M3 - Article
C2 - 22612368
AN - SCOPUS:84874614050
VL - 84
SP - 415
EP - 422
JO - Journal of Periodontology
JF - Journal of Periodontology
SN - 0022-3492
IS - 3
ER -