Comparative study of human placental & fetal brain alkaline phosphatase

T. K. Chatterjee, P. Banerjee, S. K. Banerjee, J. J. Ghosh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Significant alkaline phosphatase activity has been found in human placenta and fetal brain tissues. The enzyme is present in cytosol as well as in microsomal fractions with a relatively higher activity in the latter fraction. The activity of the enzyme in both the tissues shows a progressive increment with gestational age of the tissues; however, the term placental tissues does not register any further increase in the enzyme activity. In contrast to fetal brain, the placental enzyme demonstrates varying degrees of heat-stability depending on the gestational age of the tissue with preponderance after 14 weeks. Both fetal brain and placental enzymes demonstrate a characteristic activation after pre-exposure at pH 5.0; however, after 14 weeks the placental enzyme cannot demonstrate such acid-pH-induced activation. Dialysed enzyme preparation of both the tissues can demonstrate acid-pH-induced activation phenomena only when the pre-exposure is carried out in the presence of inorganic orthophosphate where arsenate acts as an inhibitor.

Original languageEnglish (US)
Pages (from-to)268-272
Number of pages5
JournalIndian Journal of Biochemistry and Biophysics
Volume16
Issue number5
StatePublished - Dec 1 1979

Fingerprint

Alkaline Phosphatase
Brain
Tissue
Enzymes
Chemical activation
Gestational Age
Acids
Enzyme activity
Cytosol
Placenta
Phosphates
Fetus
Hot Temperature

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Chatterjee, T. K., Banerjee, P., Banerjee, S. K., & Ghosh, J. J. (1979). Comparative study of human placental & fetal brain alkaline phosphatase. Indian Journal of Biochemistry and Biophysics, 16(5), 268-272.

Comparative study of human placental & fetal brain alkaline phosphatase. / Chatterjee, T. K.; Banerjee, P.; Banerjee, S. K.; Ghosh, J. J.

In: Indian Journal of Biochemistry and Biophysics, Vol. 16, No. 5, 01.12.1979, p. 268-272.

Research output: Contribution to journalArticle

Chatterjee, TK, Banerjee, P, Banerjee, SK & Ghosh, JJ 1979, 'Comparative study of human placental & fetal brain alkaline phosphatase', Indian Journal of Biochemistry and Biophysics, vol. 16, no. 5, pp. 268-272.
Chatterjee TK, Banerjee P, Banerjee SK, Ghosh JJ. Comparative study of human placental & fetal brain alkaline phosphatase. Indian Journal of Biochemistry and Biophysics. 1979 Dec 1;16(5):268-272.
Chatterjee, T. K. ; Banerjee, P. ; Banerjee, S. K. ; Ghosh, J. J. / Comparative study of human placental & fetal brain alkaline phosphatase. In: Indian Journal of Biochemistry and Biophysics. 1979 ; Vol. 16, No. 5. pp. 268-272.
@article{49f64d15583e41cbb6e640040e92f37c,
title = "Comparative study of human placental & fetal brain alkaline phosphatase",
abstract = "Significant alkaline phosphatase activity has been found in human placenta and fetal brain tissues. The enzyme is present in cytosol as well as in microsomal fractions with a relatively higher activity in the latter fraction. The activity of the enzyme in both the tissues shows a progressive increment with gestational age of the tissues; however, the term placental tissues does not register any further increase in the enzyme activity. In contrast to fetal brain, the placental enzyme demonstrates varying degrees of heat-stability depending on the gestational age of the tissue with preponderance after 14 weeks. Both fetal brain and placental enzymes demonstrate a characteristic activation after pre-exposure at pH 5.0; however, after 14 weeks the placental enzyme cannot demonstrate such acid-pH-induced activation. Dialysed enzyme preparation of both the tissues can demonstrate acid-pH-induced activation phenomena only when the pre-exposure is carried out in the presence of inorganic orthophosphate where arsenate acts as an inhibitor.",
author = "Chatterjee, {T. K.} and P. Banerjee and Banerjee, {S. K.} and Ghosh, {J. J.}",
year = "1979",
month = "12",
day = "1",
language = "English (US)",
volume = "16",
pages = "268--272",
journal = "Indian Journal of Biochemistry and Biophysics",
issn = "0301-1208",
publisher = "National Institute of Science Communication and Information Resources (NISCAIR)",
number = "5",

}

TY - JOUR

T1 - Comparative study of human placental & fetal brain alkaline phosphatase

AU - Chatterjee, T. K.

AU - Banerjee, P.

AU - Banerjee, S. K.

AU - Ghosh, J. J.

PY - 1979/12/1

Y1 - 1979/12/1

N2 - Significant alkaline phosphatase activity has been found in human placenta and fetal brain tissues. The enzyme is present in cytosol as well as in microsomal fractions with a relatively higher activity in the latter fraction. The activity of the enzyme in both the tissues shows a progressive increment with gestational age of the tissues; however, the term placental tissues does not register any further increase in the enzyme activity. In contrast to fetal brain, the placental enzyme demonstrates varying degrees of heat-stability depending on the gestational age of the tissue with preponderance after 14 weeks. Both fetal brain and placental enzymes demonstrate a characteristic activation after pre-exposure at pH 5.0; however, after 14 weeks the placental enzyme cannot demonstrate such acid-pH-induced activation. Dialysed enzyme preparation of both the tissues can demonstrate acid-pH-induced activation phenomena only when the pre-exposure is carried out in the presence of inorganic orthophosphate where arsenate acts as an inhibitor.

AB - Significant alkaline phosphatase activity has been found in human placenta and fetal brain tissues. The enzyme is present in cytosol as well as in microsomal fractions with a relatively higher activity in the latter fraction. The activity of the enzyme in both the tissues shows a progressive increment with gestational age of the tissues; however, the term placental tissues does not register any further increase in the enzyme activity. In contrast to fetal brain, the placental enzyme demonstrates varying degrees of heat-stability depending on the gestational age of the tissue with preponderance after 14 weeks. Both fetal brain and placental enzymes demonstrate a characteristic activation after pre-exposure at pH 5.0; however, after 14 weeks the placental enzyme cannot demonstrate such acid-pH-induced activation. Dialysed enzyme preparation of both the tissues can demonstrate acid-pH-induced activation phenomena only when the pre-exposure is carried out in the presence of inorganic orthophosphate where arsenate acts as an inhibitor.

UR - http://www.scopus.com/inward/record.url?scp=0018613527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018613527&partnerID=8YFLogxK

M3 - Article

VL - 16

SP - 268

EP - 272

JO - Indian Journal of Biochemistry and Biophysics

JF - Indian Journal of Biochemistry and Biophysics

SN - 0301-1208

IS - 5

ER -