Comparative study of susceptibilities of germinated and ungerminated conidia of Aspergillus fumigatus to various antifungal agents

Elias Kurian Manavathu, Jessica Cutright, Pranatharthi H. Chandrasekar

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Conidia are used as inocula for the in vitro susceptibility testing of Aspergillus fumigatus. Since the MIC is defined on the basis of visible mycelial growth, conidia should germinate and produce sporelings (germinated conidia) for monitoring of the growth inhibition and fungicidal activity of a drug. If a compound is capable of inhibiting germination of conidia while affecting or not affecting the growth of the organism, the MIC obtained will be the concentration of the drug required for the inhibition of conidial germination but not necessarily that required for inhibition of the growth of the organism. We investigated the susceptibility of germinated and ungerminated conidia to amphotericin B, itraconazole, voriconazole, and SCH56592. The MICs of various antifungal agents for germinated conidia were almost identical to those obtained for ungerminated conidia. In addition, both the germinated and ungerminated conidia were killed with almost equal efficiency by all of the compounds tested when exposed to the drugs for 24 h. These results suggest that either germinated or ungerminated conidia could be used as inocula for in vitro susceptibility studies of A. fumigatus with identical results.

Original languageEnglish (US)
Pages (from-to)858-861
Number of pages4
JournalJournal of clinical microbiology
Volume37
Issue number3
StatePublished - Mar 1 1999

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Aspergillus fumigatus
Fungal Spores
Antifungal Agents
Growth
Germination
Pharmaceutical Preparations
Itraconazole
Amphotericin B

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Comparative study of susceptibilities of germinated and ungerminated conidia of Aspergillus fumigatus to various antifungal agents. / Manavathu, Elias Kurian; Cutright, Jessica; Chandrasekar, Pranatharthi H.

In: Journal of clinical microbiology, Vol. 37, No. 3, 01.03.1999, p. 858-861.

Research output: Contribution to journalArticle

Manavathu, Elias Kurian ; Cutright, Jessica ; Chandrasekar, Pranatharthi H. / Comparative study of susceptibilities of germinated and ungerminated conidia of Aspergillus fumigatus to various antifungal agents. In: Journal of clinical microbiology. 1999 ; Vol. 37, No. 3. pp. 858-861.
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