Comparison of Peptides Bound to Spleen and Thymus Class II

Philippa Marrack, Leszek Ignatowicz, John W. Kappler, Joel Boymel, John H. Freed

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

In the past we and others have suggested that positive selection of developing thymocytes may depend upon interaction between the as receptors on these cells and major histocompatibility complex (MHC) proteins bound to peptides found uniquely in the selecting tissue, thymus cortical epithelium. To test this hypothesis, peptides were isolated from MHC class II proteins ofspleen, thymus cortical plus medullary epithelium, or thymus cortical epithelium alone. The results showed that the major peptides bound to class II on thymus cortical epithelium were also associated with spleen class II. Some peptides could onlybe detected in isolates from spleen, probably because of differences in the distribution or uptake of the donor proteins between spleen and thymus. Thus, although we found some tissue-specific distribution of self-peptides, our data suggest that there are no fundamental differences among these tissues in the occupancy of class II MHC by self-peptides. These results limit hypotheses which depend on a specialized mechanism of peptide generation and/or MHC class II loading to account for the positive selection ofT cells on thymic cortical epithelium.

Original languageEnglish (US)
Pages (from-to)2173-2183
Number of pages11
JournalJournal of Experimental Medicine
Volume178
Issue number6
DOIs
StatePublished - Dec 1 1993

Fingerprint

Thymus Gland
Spleen
Peptides
Major Histocompatibility Complex
Epithelium
Proteins
Tissue Distribution
Thymocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Marrack, P., Ignatowicz, L., Kappler, J. W., Boymel, J., & Freed, J. H. (1993). Comparison of Peptides Bound to Spleen and Thymus Class II. Journal of Experimental Medicine, 178(6), 2173-2183. https://doi.org/10.1084/jem.178.6.2173

Comparison of Peptides Bound to Spleen and Thymus Class II. / Marrack, Philippa; Ignatowicz, Leszek; Kappler, John W.; Boymel, Joel; Freed, John H.

In: Journal of Experimental Medicine, Vol. 178, No. 6, 01.12.1993, p. 2173-2183.

Research output: Contribution to journalArticle

Marrack, P, Ignatowicz, L, Kappler, JW, Boymel, J & Freed, JH 1993, 'Comparison of Peptides Bound to Spleen and Thymus Class II', Journal of Experimental Medicine, vol. 178, no. 6, pp. 2173-2183. https://doi.org/10.1084/jem.178.6.2173
Marrack P, Ignatowicz L, Kappler JW, Boymel J, Freed JH. Comparison of Peptides Bound to Spleen and Thymus Class II. Journal of Experimental Medicine. 1993 Dec 1;178(6):2173-2183. https://doi.org/10.1084/jem.178.6.2173
Marrack, Philippa ; Ignatowicz, Leszek ; Kappler, John W. ; Boymel, Joel ; Freed, John H. / Comparison of Peptides Bound to Spleen and Thymus Class II. In: Journal of Experimental Medicine. 1993 ; Vol. 178, No. 6. pp. 2173-2183.
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