Comparison of restriction enzyme analysis versus pulsed-field gradient gel electrophoresis as a typing system for Torulopsis glabrata and Candida species other than C. albicans

Jose Antonio Vazquez, A. Beckley, S. Donabedian, J. D. Sobel, M. J. Zervos

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Candida species have recently emerged as important nosocomial pathogens. Because of the lack of a reliable system for detecting differences within the same species, little is known about the epidemiology of infection with Candida species. We describe a typing system for Torulopsis glabrata and the non-C. albicans Candida species that uses contour-clamped homogeneous electric field electrophoresis (CHEF), a version of pulsed-field gradient gel electrophoresis, and compared it with restriction enzyme analysis (REA) of genomic DNA. One hundred seventeen clinical isolates from 40 patients were evaluated. CHEF and REA were performed on each of the isolates, and the results of the two procedures were compared. The REA procedure revealed 8 different types of Candida lusitaniae, 20 of Torulopsis glabrata, 5 of Candida tropicalis, 3 of Candida parapsilosis, and 7 of Candida kefyr, whereas the CHEF method revealed 14 different types of C. lusitaniae, 16 of T. glabrata, 10 of C. tropicalis, 10 of C. parapsilosis, and 7 of C. kefyr. The CHEF technique yielded unique patterns of electrophoretic karyotypes that could be used to distinguish intraspecies variations. When compared with REA, CHEF demonstrated greater sensitivity in recognizing subtle strain-to-strain variations in most isolates and will be a useful epidemiologic tool for studying non-C. albicans Candida species and T. glabrata.

Original languageEnglish (US)
Pages (from-to)2021-2030
Number of pages10
JournalJournal of Clinical Microbiology
Volume31
Issue number8
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Candida glabrata
Restriction Mapping
Pulsed Field Gel Electrophoresis
Candida
Electrophoresis
Candida tropicalis
Candida albicans
Karyotype
Epidemiology
DNA
Infection

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Comparison of restriction enzyme analysis versus pulsed-field gradient gel electrophoresis as a typing system for Torulopsis glabrata and Candida species other than C. albicans. / Vazquez, Jose Antonio; Beckley, A.; Donabedian, S.; Sobel, J. D.; Zervos, M. J.

In: Journal of Clinical Microbiology, Vol. 31, No. 8, 01.01.1993, p. 2021-2030.

Research output: Contribution to journalArticle

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