Compensatory anion currents in Kv1.3 channel-deficient thymocytes

Pandelakis Koni, Rajesh Khanna, Martin C. Chang, Michael D. Tang, Leonard K. Kaczmarek, Lyanne C. Schlichter, Richard A. Flavell

Research output: Contribution to journalArticle

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Abstract

Kv1.3 is a voltage-gated potassium channel with roles in human T cell activation/proliferation, cell-mediated cytotoxicity, and volume regulation and is thus a target for therapeutic control of T cell responses. Kv1.3 is also present in some mouse thymocyte subsets and splenocytes, but its role in the mouse is less well understood. We report the generation and characterization of Kv1.3-deficient (Kv1.3-/-) mice. In contrast to wild-type cells, the majority of Kv1.3-/- thymocytes had no detectable voltage-dependent potassium current, although RNA and protein for several potassium channel subunits were found in the thymocyte population. Surprisingly, the level of chloride current in the Kv1.3-/- thymocytes was increased approximately 50-fold over that in wild-type cells. There were no abnormalities in lymphocyte types or absolute numbers in thymus, spleen, and lymph nodes and no obvious defect in thymocyte apoptosis or T cell proliferation in the Kv1.3-/- animals. The compensatory effects of the enhanced chloride current may account for the apparent lack of immune system defects in Kv1.3 -/- mice.

Original languageEnglish (US)
Pages (from-to)39443-39451
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number41
DOIs
StatePublished - Oct 10 2003

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T-cells
Thymocytes
Anions
Cell proliferation
Chlorides
Voltage-Gated Potassium Channels
Thymus
Defects
T-Lymphocytes
Lymphocytes
Immune system
Potassium Channels
Cytotoxicity
Cell Proliferation
Potassium
Animals
Chemical activation
RNA
Apoptosis
Thymus Gland

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Koni, P., Khanna, R., Chang, M. C., Tang, M. D., Kaczmarek, L. K., Schlichter, L. C., & Flavell, R. A. (2003). Compensatory anion currents in Kv1.3 channel-deficient thymocytes. Journal of Biological Chemistry, 278(41), 39443-39451. https://doi.org/10.1074/jbc.M304879200

Compensatory anion currents in Kv1.3 channel-deficient thymocytes. / Koni, Pandelakis; Khanna, Rajesh; Chang, Martin C.; Tang, Michael D.; Kaczmarek, Leonard K.; Schlichter, Lyanne C.; Flavell, Richard A.

In: Journal of Biological Chemistry, Vol. 278, No. 41, 10.10.2003, p. 39443-39451.

Research output: Contribution to journalArticle

Koni, P, Khanna, R, Chang, MC, Tang, MD, Kaczmarek, LK, Schlichter, LC & Flavell, RA 2003, 'Compensatory anion currents in Kv1.3 channel-deficient thymocytes', Journal of Biological Chemistry, vol. 278, no. 41, pp. 39443-39451. https://doi.org/10.1074/jbc.M304879200
Koni P, Khanna R, Chang MC, Tang MD, Kaczmarek LK, Schlichter LC et al. Compensatory anion currents in Kv1.3 channel-deficient thymocytes. Journal of Biological Chemistry. 2003 Oct 10;278(41):39443-39451. https://doi.org/10.1074/jbc.M304879200
Koni, Pandelakis ; Khanna, Rajesh ; Chang, Martin C. ; Tang, Michael D. ; Kaczmarek, Leonard K. ; Schlichter, Lyanne C. ; Flavell, Richard A. / Compensatory anion currents in Kv1.3 channel-deficient thymocytes. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 41. pp. 39443-39451.
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