Complete genome sequence and analysis of the industrial Saccharomyces cerevisiae strain N85 used in Chinese rice wine production

Weiping Zhang, Yudong Li, Yiwang Chen, Sha Xu, Guocheng Du, Huidong Shi, Jingwen Zhou, Jian Chen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Chinese rice wine is a popular traditional alcoholic beverage in China, while its brewing processes have rarely been explored. We herein report the first gapless, near-finished genome sequence of the yeast strain Saccharomyces cerevisiae N85 for Chinese rice wine production. Several assembly methods were used to integrate Pacific Bioscience (PacBio) and Illumina sequencing data to achieve high-quality genome sequencing of the strain. The genome encodes more than 6,000 predicted proteins, and 238 long non-coding RNAs, which are validated by RNA-sequencing data. Moreover, our annotation predicts 171 novel genes that are not present in the reference S288c genome. We also identified 65,902 single nucleotide polymorphisms and small indels, many of which are located within genic regions. Dozens of larger copy-number variations and translocations were detected, mainly enriched in the subtelomeres, suggesting these regions may be related to genomic evolution. This study will serve as a milestone in studying of Chinese rice wine and related beverages in China and in other countries. It will help to develop more scientific and modern fermentation processes of Chinese rice wine, and explore metabolism pathways of desired and harmful components in Chinese rice wine to improve its taste and nutritional value.

Original languageEnglish (US)
Pages (from-to)297-306
Number of pages10
JournalDNA Research
Volume25
Issue number3
DOIs
StatePublished - Jan 1 2018

Fingerprint

Wine
Sequence Analysis
Saccharomyces cerevisiae
Genome
China
Long Noncoding RNA
RNA Sequence Analysis
Alcoholic Beverages
Beverages
Nutritive Value
Fermentation
Single Nucleotide Polymorphism
Yeasts
Oryza
Genes

Keywords

  • Annotation
  • Genome sequence
  • Rice wine yeast
  • Transcriptomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Complete genome sequence and analysis of the industrial Saccharomyces cerevisiae strain N85 used in Chinese rice wine production. / Zhang, Weiping; Li, Yudong; Chen, Yiwang; Xu, Sha; Du, Guocheng; Shi, Huidong; Zhou, Jingwen; Chen, Jian.

In: DNA Research, Vol. 25, No. 3, 01.01.2018, p. 297-306.

Research output: Contribution to journalArticle

Zhang, Weiping ; Li, Yudong ; Chen, Yiwang ; Xu, Sha ; Du, Guocheng ; Shi, Huidong ; Zhou, Jingwen ; Chen, Jian. / Complete genome sequence and analysis of the industrial Saccharomyces cerevisiae strain N85 used in Chinese rice wine production. In: DNA Research. 2018 ; Vol. 25, No. 3. pp. 297-306.
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