Complete sequence of a viral nervous necrosis virus (NNV) isolated from red-spotted grouper (Epinephelus akaara) in China

Hong Liu, Yong Teng, Xiaocong Zheng, Yurong Wu, Xiayang Xie, Junqiang He, Yiyou Ye, Zhixin Wu

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

A nodavirus isolated from red-spotted grouper (Epinephelus akaara) larvae in China has been subjected to genome analysis. The full-length genome sequences of RNA1 and RNA2 were determined, and the 5′-non-coding region (NCR) and 3′NCR sequences were determined by 5′ rapid amplification of cDNA ends (RACE) and 3′RACE. RNA1 is 3,103 nt in length and contains a 982-amino-acid open reading frame (ORF) encoding protein A with a calculated molecular mass of 110. 74 kDa. RNA2 is 1,433 nt long and contains a 338-amino-acid major ORF encoding coat protein with a calculated molecular mass of 37. 059 kDa. Multiple alignment and phylogenetic analysis clearly supported including this virus in the species Redspotted grouper nervous necrosis virus, genus Betanodavirus, family Nodaviridae.

Original languageEnglish (US)
Pages (from-to)777-782
Number of pages6
JournalArchives of Virology
Volume157
Issue number4
DOIs
StatePublished - Apr 1 2012

ASJC Scopus subject areas

  • Virology

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