TY - JOUR
T1 - Complete sequence of a viral nervous necrosis virus (NNV) isolated from red-spotted grouper (Epinephelus akaara) in China
AU - Liu, Hong
AU - Teng, Yong
AU - Zheng, Xiaocong
AU - Wu, Yurong
AU - Xie, Xiayang
AU - He, Junqiang
AU - Ye, Yiyou
AU - Wu, Zhixin
PY - 2012/4/1
Y1 - 2012/4/1
N2 - A nodavirus isolated from red-spotted grouper (Epinephelus akaara) larvae in China has been subjected to genome analysis. The full-length genome sequences of RNA1 and RNA2 were determined, and the 5′-non-coding region (NCR) and 3′NCR sequences were determined by 5′ rapid amplification of cDNA ends (RACE) and 3′RACE. RNA1 is 3,103 nt in length and contains a 982-amino-acid open reading frame (ORF) encoding protein A with a calculated molecular mass of 110. 74 kDa. RNA2 is 1,433 nt long and contains a 338-amino-acid major ORF encoding coat protein with a calculated molecular mass of 37. 059 kDa. Multiple alignment and phylogenetic analysis clearly supported including this virus in the species Redspotted grouper nervous necrosis virus, genus Betanodavirus, family Nodaviridae.
AB - A nodavirus isolated from red-spotted grouper (Epinephelus akaara) larvae in China has been subjected to genome analysis. The full-length genome sequences of RNA1 and RNA2 were determined, and the 5′-non-coding region (NCR) and 3′NCR sequences were determined by 5′ rapid amplification of cDNA ends (RACE) and 3′RACE. RNA1 is 3,103 nt in length and contains a 982-amino-acid open reading frame (ORF) encoding protein A with a calculated molecular mass of 110. 74 kDa. RNA2 is 1,433 nt long and contains a 338-amino-acid major ORF encoding coat protein with a calculated molecular mass of 37. 059 kDa. Multiple alignment and phylogenetic analysis clearly supported including this virus in the species Redspotted grouper nervous necrosis virus, genus Betanodavirus, family Nodaviridae.
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U2 - 10.1007/s00705-011-1187-5
DO - 10.1007/s00705-011-1187-5
M3 - Article
C2 - 22270757
AN - SCOPUS:84859083874
SN - 0304-8608
VL - 157
SP - 777
EP - 782
JO - Archives of Virology
JF - Archives of Virology
IS - 4
ER -