Complex I dysfunction underlies the glycolytic switch in pulmonary hypertensive smooth muscle cells

Ruslan Rafikov, Xutong Sun, Olga Rafikova, Mary Louise Meadows, Ankit A. Desai, Zain Khalpey, Jason X.J. Yuan, Jeffrey R. Fineman, Stephen Matthew Black

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

ATP is essential for cellular function and is usually produced through oxidative phosphorylation. However, mitochondrial dysfunction is now being recognized as an important contributing factor in the development cardiovascular diseases, such as pulmonary hypertension (PH). In PH there is a metabolic change from oxidative phosphorylation to mainly glycolysis for energy production. However, the mechanisms underlying this glycolytic switch are only poorly understood. In particular the role of the respiratory Complexes in the mitochondrial dysfunction associated with PH is unresolved and was the focus of our investigations. We report that smooth muscle cells isolated from the pulmonary vessels of rats with PH (PH-PASMC), induced by a single injection of monocrotaline, have attenuated mitochondrial function and enhanced glycolysis. Further, utilizing a novel live cell assay, we were able to demonstrate that the mitochondrial dysfunction in PH-PASMC correlates with deficiencies in the activities of Complexes I-III. Further, we observed that there was an increase in mitochondrial reactive oxygen species generation and mitochondrial membrane potential in the PASMC isolated from rats with PH. We further found that the defect in Complex I activity was due to a loss of Complex I assembly, although the assembly of Complexes II and III were both maintained. Thus, we conclude that loss of Complex I assembly may be involved in the switch of energy metabolism in smooth muscle cells to glycolysis and that maintaining Complex I activity may be a potential therapeutic target for the treatment of PH.

Original languageEnglish (US)
Pages (from-to)278-286
Number of pages9
JournalRedox Biology
Volume6
DOIs
StatePublished - Dec 1 2015

Fingerprint

Pulmonary Hypertension
Smooth Muscle Myocytes
Muscle
Cells
Switches
Lung
Rats
Monocrotaline
Glycolysis
Assays
Reactive Oxygen Species
Oxidative Phosphorylation
Adenosine Triphosphate
Membranes
Defects
Mitochondrial Membrane Potential
Energy Metabolism
Cardiovascular Diseases
Injections
Therapeutics

Keywords

  • Electron transport chain
  • Mitochondria
  • Pulmonary hypertension
  • Warburg effect

ASJC Scopus subject areas

  • Organic Chemistry
  • Clinical Biochemistry

Cite this

Rafikov, R., Sun, X., Rafikova, O., Louise Meadows, M., Desai, A. A., Khalpey, Z., ... Black, S. M. (2015). Complex I dysfunction underlies the glycolytic switch in pulmonary hypertensive smooth muscle cells. Redox Biology, 6, 278-286. https://doi.org/10.1016/j.redox.2015.07.016

Complex I dysfunction underlies the glycolytic switch in pulmonary hypertensive smooth muscle cells. / Rafikov, Ruslan; Sun, Xutong; Rafikova, Olga; Louise Meadows, Mary; Desai, Ankit A.; Khalpey, Zain; Yuan, Jason X.J.; Fineman, Jeffrey R.; Black, Stephen Matthew.

In: Redox Biology, Vol. 6, 01.12.2015, p. 278-286.

Research output: Contribution to journalArticle

Rafikov, R, Sun, X, Rafikova, O, Louise Meadows, M, Desai, AA, Khalpey, Z, Yuan, JXJ, Fineman, JR & Black, SM 2015, 'Complex I dysfunction underlies the glycolytic switch in pulmonary hypertensive smooth muscle cells', Redox Biology, vol. 6, pp. 278-286. https://doi.org/10.1016/j.redox.2015.07.016
Rafikov, Ruslan ; Sun, Xutong ; Rafikova, Olga ; Louise Meadows, Mary ; Desai, Ankit A. ; Khalpey, Zain ; Yuan, Jason X.J. ; Fineman, Jeffrey R. ; Black, Stephen Matthew. / Complex I dysfunction underlies the glycolytic switch in pulmonary hypertensive smooth muscle cells. In: Redox Biology. 2015 ; Vol. 6. pp. 278-286.
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