Concentrations of cyclosporin A and its metabolites in human tissues postmortem

G. L. Lensmeyer, D. A. Wiebe, I. H. Carlson, Ramiah Subramanian

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We report concentrations and distribution of cyclosporine A (CsA) and individual metabolites associated with various organ tissues and whole-blood specimens collected at autopsy from seven transplant patients who received CsA therapy. Solid-phase extraction (SPE) and specific high-performance liquid chromatographic (HPLC) procedures were used to separate and quantitate the cyclosporines. Patterns of deposition were unique for the various tissue types. Metabolites M17, M1, M18, and M8 (in addition to CsA) were the principal compounds detected in significant quantities. On a per weight basis, the sum concentration of CsA and metabolites in organ tissues was up to 53 times greater than in companion whole-blood specimens. Metabolite M17 prevailed in most tissues, except in fat and pancreas, where CsA was predominant. Overall, pancreas specimens contained a greater concentration of cyclosporines (per kilogram of tissue), followed consecutively by spleen, liver, fat, kidney, lung, bone marrow, heart, and whole blood. No CsA-related compounds were detected in brain or spinal cord tissue.

Original languageEnglish (US)
Pages (from-to)110-115
Number of pages6
JournalJournal of Analytical Toxicology
Volume15
Issue number3
DOIs
StatePublished - Jan 1 1991

Fingerprint

Metabolites
Cyclosporine
metabolite
Tissue
Cyclosporins
Blood
blood
Oils and fats
fat
Pancreas
Fats
Transplants
Solid Phase Extraction
Liver
tissue
brain
bone
Autopsy
Brain
Spinal Cord

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

Cite this

Concentrations of cyclosporin A and its metabolites in human tissues postmortem. / Lensmeyer, G. L.; Wiebe, D. A.; Carlson, I. H.; Subramanian, Ramiah.

In: Journal of Analytical Toxicology, Vol. 15, No. 3, 01.01.1991, p. 110-115.

Research output: Contribution to journalArticle

Lensmeyer, G. L. ; Wiebe, D. A. ; Carlson, I. H. ; Subramanian, Ramiah. / Concentrations of cyclosporin A and its metabolites in human tissues postmortem. In: Journal of Analytical Toxicology. 1991 ; Vol. 15, No. 3. pp. 110-115.
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