Congenital cystic adenomatoid malformation of the lung (CCAM)

Evaluation of the cellular components

Raffaella A. Morotti, Joan Cangiarella, Maria C. Gutierrez, Jaishree Jagirdar, Fred Askin, Gurmukh Singh, Sherri A. Profitt, Susan E. Wert, Jeffrey A. Whitsett, M. Alba Greco

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Congenital cystic adenomatoid malformation of the lung (CCAM) is a rare congenital lesion whose pathogenesis is not well defined. It is generally accepted that the various types of CCAMs originate at different levels of the tracheobronchial tree. To further define the pathogenesis of CCAM, we evaluated the cellular composition of different CCAM types by immunohistochemistry. Twenty-two CCAMs (17 CCAM type 1, two type 2, one type 3, and two type 4) were collected. The cellular composition was determined using immunohistochemical stains for type I cell-associated antigen (T1 cell-Ag), surfactant proteins and surfactant protein precursors (SPA, SP-B, proSP-B, and proSP-C), neuroendocrine cells (GRP), Clara cells (UP-1), and the adhesion molecule CD44v6, a glycoprotein thought to be involved in cell- matrix and cell-cell interactions. Eleven fetal lungs also were analyzed to compare cytodifferentiation of the epithelial-lined cysts of the different types of CCAM with the stages of normal lung development. Our results indicate that CGAM is caused by an arrest in lung development, and, on the basis of cytodifferentiation, two major subtypes can be distinguished. One subtype consisting of CCAM types 1, 2, and 3 that shows a bronchiolar type of epithelium and a second subtype, consisting of CGAM type 4, that has an acinar-alveolar type of epithelium. Our findings also suggest that these two subtypes may arise at different stages of the branching of the bronchopulmonary tree, the first at the pseudoglandular stage and the second at the saccular stage.

Original languageEnglish (US)
Pages (from-to)618-625
Number of pages8
JournalHuman Pathology
Volume30
Issue number6
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

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Congenital Cystic Adenomatoid Malformation of Lung
Surface-Active Agents
Lung
CD5 Antigens
Epithelium
Neuroendocrine Cells
Protein Precursors
Cell Communication
Cysts
Glycoproteins
Coloring Agents
Immunohistochemistry

Keywords

  • Clara cell antigen
  • Congenital cystic adenomatoid malformation
  • Gastrin- releasing peptide
  • Lung
  • Surfactant
  • Type 1 cell-associated antigen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Morotti, R. A., Cangiarella, J., Gutierrez, M. C., Jagirdar, J., Askin, F., Singh, G., ... Greco, M. A. (1999). Congenital cystic adenomatoid malformation of the lung (CCAM): Evaluation of the cellular components. Human Pathology, 30(6), 618-625. https://doi.org/10.1016/S0046-8177(99)90084-9

Congenital cystic adenomatoid malformation of the lung (CCAM) : Evaluation of the cellular components. / Morotti, Raffaella A.; Cangiarella, Joan; Gutierrez, Maria C.; Jagirdar, Jaishree; Askin, Fred; Singh, Gurmukh; Profitt, Sherri A.; Wert, Susan E.; Whitsett, Jeffrey A.; Greco, M. Alba.

In: Human Pathology, Vol. 30, No. 6, 01.01.1999, p. 618-625.

Research output: Contribution to journalArticle

Morotti, RA, Cangiarella, J, Gutierrez, MC, Jagirdar, J, Askin, F, Singh, G, Profitt, SA, Wert, SE, Whitsett, JA & Greco, MA 1999, 'Congenital cystic adenomatoid malformation of the lung (CCAM): Evaluation of the cellular components', Human Pathology, vol. 30, no. 6, pp. 618-625. https://doi.org/10.1016/S0046-8177(99)90084-9
Morotti, Raffaella A. ; Cangiarella, Joan ; Gutierrez, Maria C. ; Jagirdar, Jaishree ; Askin, Fred ; Singh, Gurmukh ; Profitt, Sherri A. ; Wert, Susan E. ; Whitsett, Jeffrey A. ; Greco, M. Alba. / Congenital cystic adenomatoid malformation of the lung (CCAM) : Evaluation of the cellular components. In: Human Pathology. 1999 ; Vol. 30, No. 6. pp. 618-625.
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